| Project/Area Number |
23659695
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YAMANAKA RYUYA 京都府立医科大学, 医学部, 教授 (20323991)
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| Co-Investigator(Kenkyū-buntansha) |
IKEAKA Kazuhiro 自然科学研究機構, 生理学研究所, 教授 (00144527)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 中枢神経原発悪性リンパ腫 / 糖タンパク糖鎖 / 脳腫瘍 / 糖蛋白質糖鎖 / 分子標的療法 |
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| Research Abstract |
We have isolated and characterized N-linked oligo-saccharides that are significantly increased in primary central nervous system lymphoma (PCNSL) tissue and cell lines. The structures of N-linked oligosaccharides present in 3 human normal brain tissues, 34 patients with PCNSL and 1 PCNSL cell lines were analyzed by partially automated technique for the isolation and fluorescent labeling of N-linked sugar chains from glycoproteins. Characterization of the sugar chains was achieved with the use of a combination of HPLC columns and a highly sensitive fluorescence detector at femtomole levels. By collecting peaks which accounted for 0.1% or more, twenty-one different oligosaccharide structures were characterized. The amount of specific structure for PCNSL is now under investigation. Oligo-saccharides in specimens may provide a novel marker and target for the diagnosis and treatment of PCNSL, respectively.
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