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Establishment of chondrocyte induction from iPS cells using a fluorescent reporter system.

Research Project

Project/Area Number 23659704
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

SAITO Taku  東京大学, 医学部附属病院, 特任准教授 (30456107)

Co-Investigator(Kenkyū-buntansha) OGATA Naoshi  東京大学, 医学部附属病院, 講師 (10361495)
MORIZAKI Yutaka  東京大学, 医学部附属病院, 助教 (30508099)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨 / 軟骨代謝学 / 軟骨細胞 / インジケーター / iPS細胞 / 幹細胞 / 再生医療
Research Abstract

We established fluorescent reporter iPS cells using Col2a1- and Prx1-promoter, and confirmed the fluorescent signal by stimulation of retinoic acid or BMP. We used the iPS cells to screen various factors for chondrogenic differentiation, and identified insulin, TGF-beta, inhibitor of the Notch signaling and the Wnt signaling. We are researching the optimal combination of these factors to induce chondrocyte from iPS cells.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (5 results) Remarks (2 results)

  • [Journal Article] Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium2013

    • Author(s)
      Hojo H, Saito T, et al
    • Journal Title

      J Biol Chem. E-pub

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Notch signaling in chondrocytes modulatesendochondral ossification and osteoarthritis development2013

    • Author(s)
      Hosaka Y, Saito T (equally contributed), et al
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 110 Pages: 1875-80

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] -catenin Regulates PTH/PTHrP Receptor Signals and Chondrocyte Hypertrophy through Binding to Its Intracellular C-terminal Region2013

    • Author(s)
      Yano F, Saito T (equally contributed), et al
    • Journal Title

      Arthritis Rheum

      Volume: 65 Pages: 429-35

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel disease-modifying osteoarthritis drug targeting Runx12013

    • Author(s)
      Yano F, Saito T, et al
    • Journal Title

      Ann Rheum Dis

      Volume: 72 Pages: 748-53

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium.2013

    • Author(s)
      Hojo H, Saito T, et al.
    • Journal Title

      J Biol Chem.

      Volume: E-pub

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development.2013

    • Author(s)
      Hosaka Y, Saito T (equally contributed), et al.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 110 Pages: 1785-1880

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] -catenin Regulates PTH/PTHrP Receptor Signals and Chondrocyte Hypertrophy through Binding to Its Intracellular C-terminal Region.2013

    • Author(s)
      Yano F, Saito T (equally contributed), et al.
    • Journal Title

      Arthritis Rheum

      Volume: 65 Pages: 429-435

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel disease-modifying osteoarthritis drug targeting Runx1.2013

    • Author(s)
      Yano F, Saito T, et al.
    • Journal Title

      Ann Rheum Dis.

      Volume: 72 Pages: 748-753

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gli1 Protein Participates in Hedgehog-mediated Specification of Osteoblast Lineage during Endochondral Ossification2012

    • Author(s)
      Hojo H, Saito T, et al
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 17860-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] GSK-3α and GSK-3ssproteins are involved in early stages ofchondrocyte differentiation with functional redundancy through RelA protein phosphorylation2012

    • Author(s)
      Itoh S, Saito T, et al
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 29227-36

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] C/EBPss and RUNX2 cooperate to degrade cartilage with MMP-13 as the target and HIF-2α as the inducer in chondrocytes2012

    • Author(s)
      Hirata M, Saito T, et al
    • Journal Title

      Hum Mol Genet

      Volume: 21 Pages: 1111-23

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lack of a chondroprotective effect of cyclooxygenase2 inhibition in a surgically induced model of osteoarthritis in mice2012

    • Author(s)
      Fukai A, Saito T, et al
    • Journal Title

      Arthritis Rheum

      Volume: 64 Pages: 198-203

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gli1 Protein Participates in Hedgehog-mediated Specification of Osteoblast Lineage during Endochondral Ossification.2012

    • Author(s)
      Hojo H, Saito T, et al.
    • Journal Title

      J Biol Chem.

      Volume: 287 Pages: 17860-17869

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GSK-3α and GSK-3β proteins are involved in early stages of chondrocyte differentiation with functional redundancy through RelA protein phosphorylation.2012

    • Author(s)
      Itoh S, Saito T, et al.
    • Journal Title

      J Biol Chem.

      Volume: 287 Pages: 29227-29236

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C/EBPβ and RUNX2 cooperate to degrade cartilage with MMP-13 as the target and HIF-2α as the inducer in chondrocytes.2012

    • Author(s)
      Hirata M, Saito T, et al.
    • Journal Title

      Hum Mol Genet.

      Volume: 21 Pages: 1111-1123

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lack of a chondroprotective effect of cyclooxygenase 2 inhibition in a surgically induced model of osteoarthritis in mice.2012

    • Author(s)
      Fukai A, Saito T, et al.
    • Journal Title

      Arthritis Rheum.

      Volume: 64 Pages: 198-203

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] C/EBPβ and RUNX2 cooperate to degrade cartilage with MMP-13 as the target and HIF-2α as the inducer in chondrocytes2012

    • Author(s)
      Hirata M, Saito T, et al.
    • Journal Title

      Hum Mol Genet.

      Volume: 21 Pages: 1111-23

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 軟骨分化モニタリングのためのCol2a1-EGFP-iPS細胞の樹立2013

    • Author(s)
      齋藤琢
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-23
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 変形性関節症の分子メカニズムの解明2013

    • Author(s)
      齋藤琢
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-22
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Transcriptional induction of ADAMTS5 by NF-κΒ family member RelA/p65 in chondrocytes during osteoarthritis development2011

    • Author(s)
      Kobayashi H, Saito T, et al.
    • Organizer
      International Osteoporosis Federation / Australian and New Zealand Bone & Mineral Society(IOF/ANZBMS)
    • Place of Presentation
      Gold Coast Australia
    • Related Report
      2011 Research-status Report
  • [Presentation] Functional Redundancy of GSK-3α and GSK-3β to Control Chondrocyte Differentiation through Phosphorylation of RelA/NF-κB p652011

    • Author(s)
      Itoh S, Saito T, et al.
    • Organizer
      The American Society for Bone and Mineral Research(ASBMR)
    • Place of Presentation
      San Diego USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Notch/Rbpj/Hes1 Signal in Chondrocytes Modulates the Terminal Stage of Endochondral Ossification during Skeletal Growth and Osteoarthritis Development2011

    • Author(s)
      Hosaka Y, Saito T, et al.
    • Organizer
      2011 World Congress on Osteoarthritis
    • Place of Presentation
      San Diego USA
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.u-tokyo-ortho.jp/

    • Related Report
      2012 Final Research Report
  • [Remarks] 東京大学 整形外科・脊椎外科

    • URL

      http://www.u-tokyo-ortho.jp/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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