Establishment and application of in-vitro hypertrophic and degeneration model designed to clarify the mechanism of mechanical signal transduction in chondrocytes.

• Project/Area Number: 23659705
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: CHIKUDA Hirotaka 東京大学, 大学院・医学系研究科, 講師 (30345219)
• Co-Investigator(Kenkyū-buntansha): MATSUBARA Takehiro 東京大学, 医学部附属病院, 助教 (40361498)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨 / 軟骨代謝学 / 軟骨細胞肥大分化 / メカニカルストレス / 変形性関節症

Research Abstract

The purpose of this study is to establish in-vitro hypertrophic and degeneration model designed to clarify the mechanism of mechanical signal transduction in chondrocytes. The examination of expressions of degeneration markers of chondrocytes under various conditions of hydrostatic pressure revealed that pressure of 20MPa for 1 hour was the optimum stimulus condition to induce degenerative change in mouse chondrogenic ATDC5 cells and mouse primary articular chondrocytes.