Clarification of molecular basis underlying acceleration of bone formation
| Project/Area Number |
23659712
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IMAI Yuuki 東京大学, 分子細胞生物学研究所, 特任講師 (10423873)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 骨 / 骨粗鬆症 / 骨細胞 / エピゲノム / 骨形成 |
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| Research Abstract |
MC3T3E1 cells treated with differentiation medium could not express osteocyte marker genes. Therefore, we focused on epigenetic regulation during osteocyte differentiation. ChIP analyses revealed that methylation levels of H3K4 and K27 on transcriptional start sites of osteocyte marker genes were bivalent status, suggesting that demethylation of H3K27 might to be essential for the differentiation from osteoblast to osteocyte. To examine physiological impact of this phenomenon, we generated flox mice of Kdm6A, demethylace for H3K27.
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Report
(3 results)
Research Products
(56 results)
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[Journal Article] Vitamin D receptor in osteoblasts is a negative regulator of bone mass control2013
Author(s)
Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K,Sato T, Kawano H, Aihara K, Nakamichi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S,Imai Y.
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Journal Title
Endocrinology
Volume: 154(3)
Pages: 1008-20
Related Report
Peer Reviewed
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[Journal Article] Vitamin D Receptor in osteoblasts Is a negative regulator of bone mass control2013
Author(s)
Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K, Sato T, Kawano H, Aihara K, Naka michi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S, Imai Y.
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Journal Title
Endocrinology
Volume: 154
Pages: 1008-1020
DOI
Related Report
Peer Reviewed
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[Journal Article] JMJD5, a JmjC-domain-containingprotein, negatively regulates osteoclastogenesis through facilitating NFATc1 protein degradation.2012
Author(s)
Youn MY, Yokoyama A, Fujiyama- Nakamura S, Ohtake F, Minehata KI, Yasuda H, SuzukiT, Kato S, Imai Y.
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Journal Title
J Biol Chem
Volume: 87(16)
Pages: 12994-3004
Related Report
Peer Reviewed
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[Journal Article] JMJD5, a JmjC-domain-containing protein, negatively regulates osteoclastogenesis through facilitating NFATc1 protein degradation.2012
Author(s)
Youn MY, Yokoyama A, Fujiyama-Nakamura S, Ohtake F, Minehata KI, Yasuda H, Suzuki T, Kato S, Imai Y.
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Journal Title
J Biol Chem.
Volume: Epub
Related Report
Peer Reviewed
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[Journal Article] GlcNAcylation of histone H2B facilitates its monoubiquitination2011
Author(s)
Fujiki R, Hashiba W, Sekine H, Yokoyama A, Chikanishi T, Ito S, Imai Y, Kim JH, He HH, Igarashi K,Kanno J, Ohtake F, Kitagawa H, Roeder R, Brown M, Kato S
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Journal Title
Nature
Volume: 480(7378)
Pages: 557-60
Related Report
Peer Reviewed
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[Journal Article] Signal-sensing activation of a histone lysine demethylase complex2011
Author(s)
Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S
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Journal Title
Nat Cell Biol
Volume: 13(6)
Pages: 669-76
Related Report
Peer Reviewed
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[Journal Article] O-GlcNAcylation of histone H2B facilitates its monoubiquitination2011
Author(s)
Fujiki R, Hashiba W, Sekine H, Yokoyama A, Chikanishi T, Ito S, Imai Y, Kim J, He HH, Igarashi K, Kanno J, Ohtake F, Kitagawa H, Roeder RG, Brown M, Kato S
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Journal Title
Nature
Volume: 480(7378)
Pages: 557-560
DOI
Related Report
Peer Reviewed
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[Journal Article] Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice.2011
Author(s)
Okamoto,M.,Murai,J.,Imai,Y.,Ikegami,D.,Kamiya,N.,Kato,S.,Mishita,Y.,Yoshikawa,H.and Tsumaki,N
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Journal Title
J Bone Miner Res
Volume: 26巻
Pages: 2511-22
DOI
Related Report
Peer Reviewed
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[Journal Article] Signal-sensing activation of a histone lysine demethylase complex2011
Author(s)
Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S.
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Journal Title
Nat Cell Biol
Volume: 13
Pages: 669-76
Related Report
Peer Reviewed
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