A study on the transcriptional regulation of type II collagen and aggrecan

• Project/Area Number: 23659713
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SHINOMURA Tamayuki 東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (70206118)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 軟骨 / コラーゲン / アグリカン / エンハンサー / 転写制御 / II型コラーゲン / II 型コラーゲン / 国際情報交流・USA

Research Abstract

Type II collagen and aggrecan which are the major components of cartilage occupy more than 50% of the tissue dry weight. In this study, the transcriptional mechanism that controls the high level expression of these two molecules in chondrocytes was analyzed. As a result, I found new enhancer elements that areresponsible for the regulation of each gene expression.

Research Products

• [Journal Article] A Newly Identified Enhancer Element Responsible for Type II Collagen Gene Expression (2012)
  • Author(s): Shinomura, T., Ito, K., Hook, M., and Kimura, J. H
  • Journal Title: J. Biochem Volume: 152 Pages: 565-575
  • NAID: 10031166175
  • Peer Reviewed
• [Journal Article] Versican V1 isoform regulates cell-associated matrix formation and cell behavior differentially from aggrecan in Swarm rat chondrosarcoma cells (2012)
  • Author(s): Wasa J., Nishida Y., Shinomura T.,Isogai Z., Futamura1 N., Urakawa H., Arai E., Kozawa E., Tsukushi S., and Ishiguro N
  • Journal Title: Int. J. Cancer Volume: 130 Pages: 2271-2281
  • Peer Reviewed
• [Journal Article] A newly identified enhancer element responsible for type II collagen gene expression (2012)
  • Author(s): Tamayuki Shinomura
  • Journal Title: J. Biochem. Volume: 152 Issue: 6 Pages: 565-575
  • DOI: 10.1093/jb/mvs110
  • NAID: 10031166175
  • Peer Reviewed
• [Journal Article] Versican V1 isoform regulates cell-associated matrix formation and cell behavior differentially from aggrecan in Swarm rat chondrosarcoma cells. (2012)
  • Author(s): Wasa J, Nishida Y, Shinomura T, Isogai Z, Futamura N, Urakawa H, Arai E, Kozawa E, Tsukushi S, Ishiguro N.
  • Journal Title: Int J Cancer. Volume: 130 Issue: 10 Pages: 2271-81
  • DOI: 10.1002/ijc.26230
  • Peer Reviewed
• [Presentation] アグリカンの高レベル発現に必要な新たなエンハンサー配列 (2013)
  • Author(s): 池田裕一、伊藤和生、篠村多摩之
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] アグリカンの高レベル発現に必要な新たなエンハンサー配列 (2013)
  • Author(s): 池田裕一
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] II型コラーゲンの高レベル発現に必要な新たなエンハンサー配列の存在について (2012)
  • Author(s): 伊藤和生、池田裕一、篠村多摩之
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
• [Presentation] アグリカンの高レベル発現に必要な新たなエンハンサー配列について (2012)
  • Author(s): 池田裕一、伊藤和生、篠村多摩之
  • Organizer: 第44回日本結合組織学会
  • Place of Presentation: 東京
• [Presentation] アグリカンの高レベル発現に必要な新たなエンハンサー配列について (2012)
  • Author(s): 池田裕一、大城暁子、和泉雄一、篠村多摩之
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 福島
• [Presentation] アグリカンの高レベルな発現には特別なエンハンサーが必要らしい (2012)
  • Author(s): 池田裕一
  • Organizer: 日本結合組織学会
  • Place of Presentation: 東京
• [Presentation] II 型コラーゲンの高レベル発現に必要な新たなエンハンサー配列の存在について
  • Author(s): 伊藤和生、池田裕一、篠村多摩之
  • Organizer: 軟骨代謝学会
  • Place of Presentation: 名古屋