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A study of bone atrophy treatment by detection of factors in osteocytes involved in mechanical stress

Research Project

Project/Area Number 23659717
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionKyoto University

Principal Investigator

AKIYAMA Haruhiko  京都大学, 医学研究科, 准教授 (60402830)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨細胞 / メカノセンサー / イオンチャネル / 廃用性骨萎縮 / 遺伝子改変マウス / マイクロアレイ
Research Abstract

Genome-wide screening using osteocytes in disused bone showed upregulation of BK channel genes. RT-PCR revealed that osteocytes express KCNMB1, 2 and 4 subunit genes. CaK-GFP transgenic mice were generated, and then Col1a1-mRFP1; CaK-GFP double hemi-transgenic mice were produced. We are trying live imaging of osteoblasts and osteclasts in these mice using confocal microscope when BK channel opener or blocker reagents are administered.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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