Analysis for the mechanism of the repair from injury of bone, cartilage and ligament using fluid microRNAs

• Project/Area Number: 23659719
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: YOSHITOMI Hiroyuki 京都大学, 医学研究科, 准教授 (50402920)
• Co-Investigator(Kenkyū-buntansha): ITO Hiromu 京都大学, 医学研究科, 准教授 (70397537)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マイクロRNA / 骨折治療 / バイオマーカー / 分泌マイクロRNA / 骨・軟骨・靭帯

Research Abstract

We identified six microRNAs (miRs) that were significantly changed in the plasma of patients with a bone fracture compared to that of healthy volunteers (HVs). Among these, the plasma level of miR-D significantly decreased 24 h after the fracture. To investigate the possible involvement of miR-D in the bone repair, we administrated anti-miR-D nucleotides to the model mice of bone fractures. Callus formation and neovascularization were increased in the treated mice. This study demonstrated a new role of miRNAs in the process of bone repair and a possibility of an alternative treatment for bone fracture.

Research Products

• [Journal Article] Three groups in the 28 joints for rheumatoid arthritis synovitis--analysis using more than 17,000 assessments in the KURAMA database (2013)
  • Author(s): Terao C, Hashimoto M, Yamamoto K, Murakami K, Ohmura K, Nakashima R, Yamakawa N, Yoshifuji H, Yukawa N, Kawabata D, Usui T, Yoshitomi H, Furu M, Yamada R, Matsuda F, Ito H, Fujii T, Mimori T
  • Journal Title: PLoS One Volume: 8(3) Issue: 3 Pages: e59341-e59341
  • DOI: 10.1371/journal.pone.0059341
  • NAID: 120005244457
  • Peer Reviewed
• [Journal Article] Stromal cell-derived factor 1 regulates the actin organization of chondrocytes and chondrocyte hypertrophy. (2012)
  • Author(s): Murata, K.
  • Journal Title: PLoS One. Volume: 7 Issue: 5 Pages: 237-44
  • DOI: 10.1371/journal.pone.0037163
  • Peer Reviewed
• [Journal Article] Lectin-like oxidized low-density lipoprotein receptor 1 signal is a potent biomarker and therapeutic target for human rheumatoid arthritis. (2012)
  • Author(s): Ishikawa, M., Ito, H., Akiyoshi, M., Kume, N., Yoshitomi, H., Mitsuoka, H., Tanida, S., Murata, K., Shibuya, H., Kasahara, T., Kakino, A., Fujita, Y., Sawamura, T., Yasuda, T. and Nakamura, T.
  • Journal Title: Arthritis Rheum Volume: 64 Issue: 4 Pages: 1024-1034
  • DOI: 10.1002/art.33452
  • Peer Reviewed
• [Journal Article] Human Disease Genomics Working Group; Rheumatoid Arthritis (2011)
  • Author(s): Terao C, Ohmura K, Katayama M, Takahashi M, Kokubo M, Diop G, Toda Y, Yamamoto N
  • Journal Title: PLoS One Volume: 6(6) Issue: 3 Pages: 262-271
  • DOI: 10.1097/bor.0b013e32832a2e2d
  • Peer Reviewed
• [Presentation] Stromal cell-derived factor 1 regulates the actin organization of chondrocytes and chondrocyte hypertrophy. (2013)
  • Author(s): Murata K, Kitaori T,Ito H, Yoshitomi H,Furu M, Ishikawa M, Shibuya H, MatsudaS.
  • Organizer: 59th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Antonio, USA. NIRA finalist
  • Year and Date: 2013-01-26
• [Presentation] Analysis of synovial tissue with inflammation of rheumatoid arthritis as a tertiary lymphoid organ (2012)
  • Author(s): 吉富啓之、小林志緒
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-12-05
• [Presentation] Comprehensive microRNA analysis identifies miR-24, miR-26a, and miR-125a-5p as plasma biomarkers for rheumatoid arthritis. (2012)
  • Author(s): Murata K,Yoshitomi H, Ishikawa M, Shibuya H,Ito H,Matsuda S.
  • Organizer: 2012 ACR/ARHP Annual Meeting.
  • Place of Presentation: Washington DC. USA.
  • Year and Date: 2012-11-11
• [Presentation] SDF-1は軟骨細胞のアクチン細胞骨格を制御し、肥大化を促進する (2012)
  • Author(s): 村田 浩一、伊藤 宣、北折 俊之、吉富啓之 村田 浩一、伊藤 宣、北折 俊之、吉富啓之
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋.
  • Year and Date: 2012-10-27
• [Presentation] MatsudaS. Stromal cell-derived factor 1 regulates the actin organization of chondrocytes and chondrocyte hypertrophy (2012)
  • Author(s): Murata K, Kitaori T,Ito H, Yoshitomi H,Furu M, Ishikawa M, Shibuya H
  • Organizer: GCOE リトリート2012
  • Place of Presentation: 淡路
  • Year and Date: 2012-10-05
• [Presentation] A crucial role for lectin-like oxidized low-density lipoprotein receptor-1 signal in rheumatoid arthritis. (2012)
  • Author(s): Ishikawa M,Ito H, Yoshitomi H,Akiyoshi M, Murata K, Furu M, Matuda S.
  • Organizer: 15th Asia Pacific League of Associations for Rheumatology (APLAR)
  • Place of Presentation: JORDAN
  • Year and Date: 2012-09-11
• [Presentation] 生物製剤使用中患者の手術 -生物学的製剤の適正な使用と諸問題- (2012)
  • Author(s): 伊藤宣
  • Organizer: 第22回日本リウマチ学会近畿支部学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-01
• [Presentation] Stromal cell-derived factor 1 regulates the actin organization of chondrocytes and chondrocyte hypertrophy (2012)
  • Author(s): 村田 浩一、北折 俊之、中村 孝志、伊藤 宣
  • Organizer: 第30回骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-20
• [Presentation] A crucial role for lectin-like oxidized LDL receptor-1 signal on synovial inflammation in RA. (2012)
  • Author(s): Ishikawa M,Ito H, Akiyoshi M, Murata K, Tanida S, Shibuya H, KasaharaY, Yoshitomi H Nakamura T.
  • Organizer: The Annual European Congress of Rheumatology EULAR2012
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2012-06-06
• [Presentation] A crucial role for lectin-like oxidized LDL receptor-1 signal on cartilage destruction in inflammatory arthritis. (2012)
  • Author(s): Ishikawa M,Ito H, Akiyoshi M, Yoshitomi H,Murata K, Tanida S, Shibuya H, Kume N, Sawamura T, Nakamura T
  • Organizer: 58th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-02-06
• [Presentation] 関節リウマチ滑膜にはIL-27産生性CD14陽性細胞が浸潤し炎症や遊走を制御する (2011)
  • Author(s): 吉富啓之、伊藤宣、中村孝志
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-28
• [Presentation] 関節疾患と分泌microRNA (2011)
  • Author(s): 吉富啓之、村田浩一、伊藤宣、中村孝志
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-10-20
• [Presentation] 生物製剤使用中患者の手術.-生物学的製剤の選択と実践 (2011)
  • Author(s): 伊藤宣
  • Organizer: 第21回日本リウマチ学会近畿支部学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2011-09-03
• [Presentation] 施行した人工関節の種類によってその後の死亡率に差があるのかー人工関節置換術後生存率調査- (2011)
  • Author(s): 伊藤宣、吉富啓之、中村伸一郎、中村孝志
  • Organizer: 第55回日本リウマチ学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2011-07-18
• [Presentation] 血漿・関節液マイクロRNAは関節リウマチの疾患バイオマーカーとなりうるか (2011)
  • Author(s): 村田浩一、吉富啓之、伊藤宣、中村孝志
  • Organizer: 第55回日本リウマチ学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2011-07-18
• [Presentation] 強直性脊椎炎に対する レミケードの使用経 (2011)
  • Author(s): 吉富 啓之 伊藤 宣 中村孝志
  • Organizer: 京整会RA懇話会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-03
• [Presentation] 関節疾患と分泌microRNA
  • Author(s): 吉富啓之
  • Organizer: 第26回 日本整形外科学会基礎学術集会(招待講演)
  • Place of Presentation: ベイシア文化ホール（前橋市）
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~seikeigeka/fundamental/nankotsu.html
• [Remarks] 京都大学整形外科
  • URL: http://www.kuhp.kyoto-u.ac.jp/~seikeigeka/fundamental/nankotsu.html