Evaluation of mechanism of ossification of the posterior longitudinal ligament and identification of candidate disease gene associated with ossification of the posterior longitudinal ligament

• Project/Area Number: 23659720
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: OKAMOTO Takeshi 京都大学, 医学研究科, 助教 (30414113)
• Co-Investigator(Kenkyū-buntansha): NEO Masashi 大阪医科大学, 医学研究科, 教授 (80311736) ; TOGUCHIDA Junya 京都大学, 再生医科学研究所, 教授 (40273502)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マイクロアレイ / 細胞・組織

Research Abstract

Human induced pluripotent stem cells were established from the patients with OPLL. We performed osteogenic induction and ligamentocyte induction from the OPLL-iPS. No difference was observed in osteblastic induction potential between the OPLL-iPS and iPS from healthy control patients. Gene expression profiling in ligamentocyte-precursor cells induced from OPLL-iPS was evaluated. Comparing gene expression profile with that of the iPS from healthy control patients, candidate genes which may be associated with mechanism of ectopic ossification of the posterior longitudinal ligament in OPLL patients were selected.