Regeneration of growth plate using mesenchymal stem cells

• Project/Area Number: 23659721
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Osaka University
• Principal Investigator: YOSHIKAWA Hideki 大阪大学, 医学(系)研究科(研究院), 教授 (60191558)
• Co-Investigator(Kenkyū-buntansha): HIGUCHI Chikahisa 大阪大学, 保健センター, 助教 (40432421)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 成長軟骨帯損傷 / 骨橋 / 間葉系細胞 / 3次元人工組織 / 変形矯正 / 成長軟骨帯 / 成長軟骨帯再生 / 早期骨端線閉鎖 / 3次元間葉系細胞由来組織 / 早期成長軟骨帯閉鎖

Research Abstract

Growth plate injuries often cause bone shortening or angular deformities of affected bones because of total or partial bony bridging between epiphysis and metaphysis. In order to explore new method of the treatment of growth plate injuries, we investigated availability of 3-dimentional artificial tissue, which is made of synovium-derived mesenchymal stem cells, for the regeneration of injured growth plate. Our results demonstrated that physis-like cartilage tissue was reorganized by implantation of 3D tissue. Moreover, we confirmed that correction of bone shortening or angular deformities was followed after the regeneration of tissue regeneration.

Research Products

• [Journal Article] Treatment of partial growth arrest using an in vitro-generated scaffold-free tissue-engineered construct derived from rabbit synovial mesenchymal stem cells (2012)
  • Author(s): Yoshida K, Higuchi C, Nakura A, Nakamura N, Yoshikawa H
  • Journal Title: J Pediatr Orthop Volume: 32(3) Issue: 3 Pages: 314-21
  • DOI: 10.1097/bpo.0b013e31824afee3
  • Peer Reviewed
• [Journal Article] Hip joint pigmented villonodular synovitis in a young girl : a case report (2012)
  • Author(s): Higuchi C, Ohno I, Yoshikawa H
  • Journal Title: Journal of Pediatric Orthopaedics B Volume: 21(4) Issue: 4 Pages: 335-8
  • DOI: 10.1097/bpb.0b013e3283524bdf
  • ISSN: 1060-152X
  • Peer Reviewed
• [Presentation] 間葉系幹細胞由来三次元人工組織(TEC)による成長軟骨修復 (2013)
  • Author(s): 樋口周久、吉田清志、中村憲正、吉川秀樹
  • Organizer: 第24回日本小児整形外科学会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-11-08
• [Presentation] Correction of knee deformity following physeal arrest with the aid of a three-dimensional computer system (2013)
  • Author(s): 樋口周久、吉田清志、村瀬剛、吉川秀樹
  • Organizer: 9^<th> Asia Pacific Orthopaedic Association Paediatric Section
  • Place of Presentation: クチン・マレーシア
• [Presentation] Treatment of growth arrest using an in vitro generated scaffold-free tissue engineered construct(TEC) derived from synovial mesenchymal stem cells (2013)
  • Author(s): 吉田清志、樋口周久、中村憲正、吉川秀樹
  • Organizer: International Society of Limb Salvage 17^<th> General Meeting
  • Place of Presentation: ボローニャ・イタリア
• [Presentation] Correction of knee deformity following physeal arrest with the aid of a three-dimensional computer system. (2013)
  • Author(s): Higuchi C, Yoshida K, Murase T, Yoshikawa H.
  • Organizer: 9th Asia Pacific Orthopaedic Association Paediatric Section Congress
  • Place of Presentation: Kuching, Malaysia
• [Presentation] 陳旧性坐骨結節骨折に対して手術加療を施行した1例 (2013)
  • Author(s): 藤本初月、樋口周久、吉田清志、吉川秀樹
  • Organizer: 第121回中部日本整形外科災害外科学会
  • Place of Presentation: 名古屋
• [Presentation] 間葉系幹細胞由来三次元人工組織（TEC)による成長軟骨修復 (2013)
  • Author(s): 樋口周久、吉田清志、吉川秀樹
  • Organizer: 第24回日本小児整形外科学会
  • Place of Presentation: 横浜
• [Presentation] Correction of severe knee deformity following physeal arrest with the aid of a three-dimensional computer system and external fixator (2012)
  • Author(s): 吉田清志、樋口周久、村瀬剛、吉川秀樹
  • Organizer: 2^<nd> World Congress on External Fixation and Bone Reconstruction
  • Place of Presentation: サルバドール・ブラジル
• [Presentation] Percutaneous Epiphysiodesis Using Transphyseal Screw (PETS)を用いて変形矯正した片肢性骨端異形成症の1例 (2011)
  • Author(s): 樋口周久、吉田清志、吉川秀樹
  • Organizer: 第26回日本小児整形外科学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-12-08
• [Presentation] 片側肥大症による脚長差に対しスクリューを用いた経皮的成長抑制術を施行した1例 (2011)
  • Author(s): 樋口周久、吉田清志、吉川秀樹
  • Organizer: 第117回中部日本整形外科災害外科学会
  • Place of Presentation: 宇部市
  • Year and Date: 2011-10-28