Challenging study of immunotherapy for osteosarcoma usingmicroparticles induced by photodynamic therapy

• Project/Area Number: 23659730
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Heian Jogakuin(St.Agnes')University
• Principal Investigator: KUSUZAKI Katsuyuki 平安女学院大学, 文化創造センター, 客員教授 (30177993)
• Co-Investigator(Kenkyū-buntansha): ASHIHARA Eishi 京都府立医科大学, 医学(系)研究科, 講師 (70275197) ; HOSOGI Shigekuni 京都府立医科大学, 医学(系)研究科, 講師 (30433254)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨肉腫 / アクリジンオレンジ / 光線力学的治療法 / 微粒子 / エキソゾーム / 免疫療法 / マクロファージ / 骨肉種 / 細胞内小器官 / がん免疫 / エクソゾーム

Research Abstract

In this study, we undertook to investigate inhibition effect oftumor growth and pulmonary metastasis on mouse osteosarcoma by immurlotherapy withmicroparticles induced by photodynamic therapy with acridine orange. Results showed thatthese microparticles including exQsomes did rlot inhibit tumor growth and pulmonarymetastasis, because they did not activate immunoactivity via macrophages

Research Products

• [Journal Article] Improvement of insulin resistance, blood pressure and interstitial pH in early developmental stage of insulin resistance in OLETF rats by intake of propolis extracts. (2013)
  • Author(s): Aoi W,Hosogi S, Niisato N, Yokoyama N, Hayata H, Miyazaki H, Kusuzaki K, Fukuda T, Fukui M, Nakamura N, Marunaka Y
  • Journal Title: BBRC Volume: 432 Pages: 650-653
  • Peer Reviewed
• [Journal Article] Can a less radical surgery using photodynamic therapy with acridine orange be equal to a wide-margin resection? (2013)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Nakamura T, Sudo A.
  • Journal Title: Clin Orthop Relat Res Volume: 471(3) Issue: 3 Pages: 792-802
  • DOI: 10.1007/s11999-012-2616-9
  • Peer Reviewed
• [Journal Article] An inhibitor of Na/H(+) exchanger, ethy1-isopropyl amiloride (EIPA), diminishes proliferation of MKN28 human gastric cancer cells by decreasirlg the cytosolic Cl(-) concentration via DIDS-sensitive pathways。 (2012)
  • Author(s): Hosogi S, Miyazaki H, Nakajima KI, Ashihara E, Niisato N, Kusuzaki K, Marunaka Y.
  • Journal Title: Cell Physiol Biochem. Volume: 30 Pages: 1241-1253
  • Peer Reviewed
• [Journal Article] Can a less radical surgery using Photodynamic therapy with acridine orange be equal to a wide-margin resection? (2012)
  • Author(s): Matsubara T, Kusuzaki K, Nanamura T, Matsumine A, Sudo A.
  • Journal Title: Clin Orthop Relat Res Volume: 471 Pages: 792-802
  • Peer Reviewed
• [Journal Article] Trarlslationalresearch of photodynamic therapy with acridine orange which targets cancer acidity. (2012)
  • Author(s): Kusuzaki K, Ashihara E, Hosogi S, Matsubara T, Satonaka H, Nanamura T, Matsumine A, Murata H, Sudo A, Uchida A, Murata H, Baldini N, Fais S, Marunaka Y.
  • Journal Title: Current Pharmaceutical Design Volume: 18 Pages: 1414-11420
  • Peer Reviewed
• [Journal Article] Translationalresearch of photodynamic therapy with acridine orange which targets cancer acidity. (2012)
  • Author(s): Kusuzaki, Katsuyuki
  • Journal Title: Current Pharmaceutical Design Volume: 18 Pages: 1414-11420
  • Peer Reviewed
• [Journal Article] .Translationalresearch of photodynamic therapy with acridine orange which targets cancer acidity. (2012)
  • Author(s): Kusuzaki K, Ashihara E, Hosogi S, Matsubara T, Satonaka H, Manamura T, Matsumine A, Murata H, Sudo A, Uchida A, Murata H, Baldini N, Fais S, Marunaka Y.
  • Journal Title: Current Pharmaceutical Design Volume: 18 Pages: 1414-11420
  • Peer Reviewed
• [Journal Article] がん細胞の酸性微小環境および酸性細胞内小器官を標的としたがん治療法の開発 (2011)
  • Author(s): 楠崎克之
  • Journal Title: CLINICAL CALCIUM Volume: 21 Pages: 61-67
  • Peer Reviewed
• [Journal Article] Acridine orange inhibits pulmonary metastasis of mouse steosarcoma. (2011)
  • Author(s): Satonaka H, Kusuzaki K, Akeda K, Tsujii M, Iino T, Uemura T, Matsubara T,Nakamura T, Asanuma K, Matsumine T, Sudo A
  • Journal Title: Anticancer Res Volume: 31 Pages: 4163-4168
  • Peer Reviewed
• [Presentation] Acridine orange therapy as a new less-invasive limb salvage surgery for rhabdomyosarcomas and synovial sarcomas (2013)
  • Author(s): Takao Matsubara.
  • Organizer: AAOS (American Association of Orthopaedic Surgeons) 2013 Annual Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] Acridine orange therapy as a new less-invasive limb salvage surgery for rhabdomyosarcomas and synovial sarcomas (2013)
  • Author(s): Matsubara, Takao
  • Organizer: American Association of Orthopaedic Surgeons 2013 Annual Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] 化学療法感受性腫瘍に対するアクリジンオレンジ療法の検討 (2012)
  • Author(s): 松原孝夫.
  • Organizer: アクリジンオレンジ治療研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-12-08
• [Presentation] A minimally invasive surgery for bone metastases using the combination of photodynamic therapy and hyperthermia treatment (2012)
  • Author(s): Takao Matsubara
  • Organizer: CTOS (Connective Tissue Oncology Society) 17しh Annual Meeting
  • Place of Presentation: Prague, Czech Republic
• [Presentation] 骨肉腫細胞における細胞外環境因子とアクリジンオレンジ腫瘍内集積濃度の関係 (2012)
  • Author(s): 松原孝夫
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] アクリジンオレンジによる多剤耐性骨肉腫細胞株の耐性克服機序の解明 (2012)
  • Author(s): 楠崎克之
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] C1-channeI/transporters as new targets for cancer therapies based on disruption of autophagy ability via modification of lysosome acidification. (2012)
  • Author(s): Hosogis
  • Organizer: 3^rd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Kyoto Japan
• [Presentation] Acidic pHe promotes chemoresistance of human osteosarcoma cells through difference survival adaptation mechanism. (2012)
  • Author(s): Avnet S.
  • Organizer: 3^rd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Kyoto Japan
• [Presentation] Carbonic anhydrase IX inhibitors as effective therapeutic tools for osteosarcoma treatment. (2012)
  • Author(s): Perut F
  • Organizer: 3^rd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Kyoto Japan
• [Presentation] A minimal invasive surgery for bone metastases using photodynamic therapy with acridine orange. (2012)
  • Author(s): Matsubara T.
  • Organizer: 3^rd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Kyoto Japan
• [Presentation] Carbonic anhydrase IX inhibitors as effective therapeutic tools for osteosarcoma treatment (2012)
  • Author(s): Perut F.
  • Organizer: 3^rd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Kyoto Japan
• [Presentation] 転移性骨腫瘍に対するアクリジンオレンジ光線力学的療法および磁性体温熱療法併用治療法の検討 (2012)
  • Author(s): 松原孝夫
  • Organizer: 71回日本癌学会学術集会
  • Place of Presentation: 札幌
• [Presentation] A new therapeutic photodynamic surgery using acridine orange for rhabdomyosarcomas and synovial sarcomas (2012)
  • Author(s): Takao Matsubara
  • Organizer: APMSTS (Asia Pacific Musculoskeletal Tumor Society) Meeting 2012
  • Place of Presentation: Kuala lumpur, Malaysia
• [Presentation] 緩和的治療としての転移性骨腫瘍に対するアクリジンオレンジ療法および磁性体温熱療法併用治療中部日本整形災害外科学会 (2012)
  • Author(s): 松原孝夫
  • Organizer: 第120回学術集会
  • Place of Presentation: 大阪
• [Presentation] A new therapeutic limb salvage surgery using acridine orange in patients with bone sarcomas (2012)
  • Author(s): Matsubara T.
  • Organizer: AAOS 2012 Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] A minimal invasive surgery for bone metastases using photodynamic therapy with acridine orange. (2012)
  • Author(s): Matsubara, Takao
  • Organizer: 3rd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Kyoto Japan
• [Presentation] A new therapeutic photodynamic surgery using acridine orange for rhabdomyosarcomas and synovial sarcomas (2012)
  • Author(s): Matsubara, Takao
  • Organizer: Asia Pacific Musculoskeletal Tumor Society 2012
  • Place of Presentation: Kuala lumpur, Malaysia
• [Presentation] A minimally invasive surgery for bone metastases using the combination of photodynamic therapy and hyperthermia treatment (2012)
  • Author(s): Matsubara, Takao
  • Organizer: Connective Tissue Oncology Society 17th Annual Meeting
  • Place of Presentation: Prague, Czech Republic
• [Presentation] Cancer microenvironment and new target cancer therapy (2011)
  • Author(s): Kusuzaki K
  • Organizer: Special Lecture in Bologna University
  • Place of Presentation: Bologna, Italy
  • Year and Date: 2011-11-22
• [Presentation] Cancer microenvironment and new target cancer therapy (2011)
  • Author(s): Kusuzaki K.
  • Organizer: Special Lecture in Florence University
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-11-21
• [Presentation] Acridine orange therapy on Cancer (2011)
  • Author(s): Kusuzaki K.
  • Organizer: Italy/Japan Joint Meetirlg on Cancer Research (co-president)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-11-04
• [Presentation] アクリジンオレンジを用いた分子イメージングの低侵襲外科手術への応用(パネルディスカッション=生体イメージング技術の骨・軟部腫瘍への応用) (2011)
  • Author(s): 楠崎克之
  • Organizer: 第44回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-15
• [Presentation] An inhibitor of Na+/H+ exchanger (NHE), ethy1-isopropyl amiloride (EIPA), diminishes proliferation of MKN 28 human gastric cancer cells by decreasing the intracellular Cl- conce「1t「atiorl via DIDS-sensitive pathway (2011)
  • Author(s): Hosogi S.
  • Organizer: 2^nd Symposium of the Internal Society for Proton Dynamics in Cancer
  • Place of Presentation: Nice France
• [Presentation] Clinica1 0utcome of Acridine Orange Therapy SupPorted by Photodynamic Surgery and Radiodynamic Therapy for Soft Tissue Sarcomas (2011)
  • Author(s): Matsubara T.
  • Organizer: Compared with Conventional Limb Salvage Surgery after Wide Resection. 16th ISOLS Meeting
  • Place of Presentation: Beigin China
• [Presentation] 悪性骨軟部腫瘍に対するアクリジンオレンジ治療法―基礎研究から臨床応用へー (2011)
  • Author(s): 楠崎克之
  • Organizer: 俱進会 神奈川県北支部 学術講演会(招待講演)
  • Place of Presentation: 横浜市
• [Book] 骨腫瘍.NEWエッセンシャ ル整形外科学,星野雄一、吉川秀樹、齋藤知行 編集 (2012)
  • Author(s): 楠崎克之
  • Publisher: 医歯薬出版株式会社
• [Book] 第3節骨肉腫.第2章: がん領域の稀少疾患の医療ニーズ.稀 少疾患/難病の診断・治療と製品開発 (2012)
  • Author(s): 楠崎克之.
  • Publisher: 医療情報教会株式会社、東京
• [Book] 稀少疾患/難病の診断・治療と製品開発 (2012)
  • Author(s): 楠崎克之
  • Total Pages: 981
  • Publisher: 医療情報教会株式会社
• [Book] In Sarcoma Edited by Bulter EJ: Nova Science Publisher Inc. (2012)
  • Author(s): Kusuzaki K, Ashihara E, Hosogi S, Matsubara T, Satonaka H, Manamura T, Matsumine A, Murata H, Sudo A, Uchida A, Murata H, Baldini N, Fais S, Marunaka Y.
  • Total Pages: 5
  • Publisher: New concept of limb salvage surgery in musculoskeletal sarcomas with acridine orange therapy