Study of Coculture System of Vascular Endothelial Cells and Bone Marrow cells

• Project/Area Number: 23659733
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: UEMURA Toshimasa 独立行政法人産業技術総合研究所, ナノシステム研究部門, 上級主任研究員 (60176641)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨再生 / 血管内皮細胞 / 骨髄細胞

Research Abstract

Tissue-engineered bone has attracted much attention as an alternative material for bone grafting; however, implantable bone tissue of an appropriate size and shape for clinical use has not yet been developed due to a lack of vascularization, which results

Research Products

• [Journal Article] Engineered bone tissue associated with vascularization utilizing a rotating wall vessel bioreactor (2013)
  • Author(s): Masanori Nishi, Rena Matsumoto, Jian Dong, Toshimasa Uemura
  • Journal Title: J Biomed Mater Res A Volume: 101(2) Pages: 421-427
• [Journal Article] Engineered bone tissue associated with vascularization utilizing a rotating wall vessel bioreactor. (2013)
  • Author(s): Masanori Nishi
  • Journal Title: J Biomed Mater Res A. Volume: 101 Pages: 421-427
  • Peer Reviewed
• [Journal Article] Effects of implantation of three-dimensional engineered bone tissue with a vascular-like structure on repair of bone defects (2012)
  • Author(s): Masanori Nishi, Rena Matsumoto, Jian Dong, Toshimasa Uemura
  • Journal Title: Applied Surface Science Volume: 262 Pages: 60-63
• [Journal Article] Effects of implantation of three dimensional engineered bone tissue (2012)
  • Author(s): M.Nishi, R.Matsumoto, J.Dong, T. Uemura
  • Journal Title: Applied Surface Science Volume: published on line Pages: 1-6
  • DOI: 10.1016/j.apsusc.2012.01.092
  • Peer Reviewed
• [Journal Article] Regeneration of Bone Tissue in a Controlled in VitroEnvironment with a Rotating Wall Vessel Bioreactor (2011)
  • Author(s): Masanori NISHI, Rena MATSUMOTO, Jian DONG, Toshimasa UEMURA
  • Journal Title: Nano Biomedicine Volume: 3(2) Pages: 267-274
  • NAID: 130004555807
• [Journal Article] Regeneration of a matured bone tissue in a controlled in vitro (2011)
  • Author(s): M.Nishi, R.Matsumoto, J.Dong, T. Uemura
  • Journal Title: Nano Biomedicine Volume: ３（２） Pages: 267-274
  • Peer Reviewed
• [Presentation] RWV微小重力培養を用いた3 次元組織の構築と再生医療 (2013)
  • Author(s): 植村寿公
  • Organizer: 第25回バイオエンジニアリング講演会
  • Year and Date: 2013-01-09
• [Presentation] RWV微小重力培養を用いた３次元組織の構築と再生医療 (2013)
  • Author(s): 植村寿公
  • Organizer: 第25回バイオエンジニアリング講演会
  • Place of Presentation: 茨城県
  • Invited
• [Presentation] ＲＷＶ回転培養を用いた血管新生を伴う三次元骨組織構築 (2013)
  • Author(s): 植村寿公
  • Organizer: 第７回ナノバイオメディカル学会
  • Place of Presentation: 京都
• [Presentation] RWVバイオリアクターによる擬微小重力培養法を用いた 3 次元硬組織の再生技術 (2012)
  • Author(s): 植村寿公
  • Organizer: 日本再生歯科医療学会設立10周年記念セミナー
  • Place of Presentation: 兵庫県
  • Year and Date: 2012-09-02
• [Presentation] RWVバイオリアクターによる擬微小重力培養法を用いた３次元硬組織の再生技術 (2012)
  • Author(s): 植村寿公
  • Organizer: 日本再生歯科医学会設立１０周年記念セミナー
  • Place of Presentation: 兵庫県
  • Invited
• [Presentation] ＲＷＶ回転培養を利用した共培養系による血管様構造を備えた移植用骨組織の構築 (2012)
  • Author(s): 西正統
  • Organizer: 第６回ナノバイオメディカル学会大会
  • Place of Presentation: つくば
• [Presentation] 骨髄細胞の自己組織化を利用した複雑な３次元骨組織構築の検討 (2012)
  • Author(s): 西正統、松本令奈、Jian Dong, 植村寿公
  • Organizer: つくば医工連携フォーラム２０１２
  • Place of Presentation: 物質・材料研究機構（茨城県）
• [Presentation] Co-culturing of bone marrow mesenchymal stem cells and endothelial cells (2011)
  • Author(s): M.Nishi, R.Matsumoto, J.Dong, T. Uemura
  • Organizer: SIB2011 Satellite Symposium in Kyoto, The Symposium on Micro/Nano Aspscts of Biomaterials
  • Place of Presentation: 京都テルサ（京都府）
• [Presentation] RWVバイオリアクターを用いた３次元組織構築法とインビトロ評価 (2011)
  • Author(s): 植村寿公
  • Organizer: 第２４回日本動物実験代替法学会学術大会(招待講演)
  • Place of Presentation: 宮城県建設産業会館（宮城県）
• [Presentation] RWV回転培養装置を用いた骨髄由来間葉系幹細胞と内皮細胞の共培養による骨組織再生に関する研究 (2011)
  • Author(s): 西正統、松本令奈、Jian Dong, 植村寿公
  • Organizer: 第３３回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ（京都府）
• [Presentation] RWV回転培養装置を用いた骨髄由来間葉系幹細胞と骨髄由来の非接着細胞の共培養系による骨再生の検討 (2011)
  • Author(s): 西正統、松本令奈、Jian Dong, 植村寿公
  • Organizer: 第３３回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ（京都府）
• [Presentation] ＲＷＶ回転培養装置を利用した共培養系による血管様構造を備えた移植用骨組織の作製に関する検討 (2011)
  • Author(s): 西正統、松本令奈、Jian Dong, 植村寿公
  • Organizer: 第４９回日本人工臓器学会大会
  • Place of Presentation: 都市センターホテル（東京都）
• [Presentation] CO-CULTURE OF BONE MARROW CELLS ANSD ENDOTHELIAL CELLS BY ROTATING WALL VESSEL(RWV) BIOREACTOR (2011)
  • Author(s): M.Nishi, R.Matsumoto, J.Dong, T. Uemura
  • Organizer: The 11th Asian BioCeramics Symposium, Tsukuba
  • Place of Presentation: つくば国際会議場（茨城県）
• [Book] Cartilage Regeneration from Bone Marrow Cells and its Automation System for Clinical Application, TISSUE ENGINEERING FOR TISSUE AND ORGAN REGENERATION ed by Daniel Eberli (2011)
  • Author(s): Toshimasa Uemura, Masanori Nishi,Kunitomo Aoki, Takashi Tsumura
  • Publisher: INTECH
• [Book] Regenerative Medicine and Tissue Engineering from Cells to Organs (Book2) (2011)
  • Author(s): T. Uemura, M. Nishi,
  • Publisher: Carilage Regeneration from Bone Marrow Cells Using RWV