Research Project
Grant-in-Aid for Challenging Exploratory Research
To develop a control method for embryonic implantation and subsequent differentiation and proliferation as well as recognition of embryonic attachment in uterus, expression pattern of genes responsible for initiation of implantation window in uterine tissues from an identical recipient which transferred fertilized oocytes at pronucleus stage and embryos at 8-cell stage into right and left side of the oviduct, respectively, was examined by using a DNA microarray. As a result, at the open of implantation window, one down-regulated and two up-regulated genes were identified. These presumably responsible genes for window of implantation were further analyzed the expression profile during the course of preimplantation development and implantation by a real time PCR method. Expression of Sprr2f was highest on 0.5 dpc and gradually decreased with preimplantation development and during implantation. However, Sprr2f strongly expressed in ovarian tissues on 3.5 dpc. Ass1 highly expressed in uterus on 4.5 dpc and in ovary on 3.5 dpc. Further analysis of these molecules as candidates of genes responsible for regulation of implantation window is required in uterine and ovarian tissues at peri-implantation period.
All 2013 2012 2011
All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (18 results)
J. Reprod. Dev
Volume: 59 Pages: 92-96
10031156817
Volume: 58 Pages: 260-263
10030754171
Theriogenology
Volume: 77 Pages: 347-355
Reprod. Dom. Anim
Volume: 47 Pages: 141-143
J. Reprod. Dev.
Reprod. Dom. Anim.
Med. Biol
Volume: 10 Pages: 43-49
Biol. Reprod
Volume: 84 Pages: 363-368
Volume: 57 Pages: 176-182
10028274982
Reprod. Med. Biol
Volume: 10 Pages: 259-266
10030444416
J.Mamm. Ova Res
Volume: 28 Pages: 143-147
10029898554
Reprod. Med. Biol.
10029042897
Biol. Reprod.
J. Mamm. Ova Res.
