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Study on the molecules responsible for implantation window

Research Project

Project/Area Number 23659767
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

SUZUKI Hiroshi  帯広畜産大学, 原虫病研究センター, 教授 (60333473)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords着床 / 胚 / 遺伝子 / 妊娠 / 偽妊娠
Research Abstract

To develop a control method for embryonic implantation and subsequent differentiation and proliferation as well as recognition of embryonic attachment in uterus, expression pattern of genes responsible for initiation of implantation window in uterine tissues from an identical recipient which transferred fertilized oocytes at pronucleus stage and embryos at 8-cell stage into right and left side of the oviduct, respectively, was examined by using a DNA microarray. As a result, at the open of implantation window, one down-regulated and two up-regulated genes were identified. These presumably responsible genes for window of implantation were further analyzed the expression profile during the course of preimplantation development and implantation by a real time PCR method. Expression of Sprr2f was highest on 0.5 dpc and gradually decreased with preimplantation development and during implantation. However, Sprr2f strongly expressed in ovarian tissues on 3.5 dpc. Ass1 highly expressed in uterus on 4.5 dpc and in ovary on 3.5 dpc. Further analysis of these molecules as candidates of genes responsible for regulation of implantation window is required in uterine and ovarian tissues at peri-implantation period.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (36 results)

All 2013 2012 2011

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (18 results)

  • [Journal Article] Sexing of dog sperm by fluorescence in situ hybridization2013

    • Author(s)
      Oi, M., Yamada, K., Hayakawa, H., Suzuki, H.
    • Journal Title

      J. Reprod. Dev

      Volume: 59 Pages: 92-96

    • NAID

      10031156817

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effect of hyperbaric oxygen therapy on the follicular survival in the mouse ovary after transplantation2012

    • Author(s)
      Hayashi, S., Asano, T., Kakizaki, R., Suzuki, H.
    • Journal Title

      J. Reprod. Dev

      Volume: 58 Pages: 260-263

    • NAID

      10030754171

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers2012

    • Author(s)
      Abdel-Ghani, M., Shimizu, T., Asano, T., Suzuki, H.
    • Journal Title

      Theriogenology

      Volume: 77 Pages: 347-355

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos and resulting pregnancies.2012

    • Author(s)
      Suzuki, H.
    • Journal Title

      Reprod. Dom. Anim

      Volume: 47 Pages: 141-143

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effect of hyperbaric oxygen therapy on the follicular survival of mouse ovary after transplantation.2012

    • Author(s)
      Hayashi, S., Asano, T., Kakizaki, R. and Suzuki, H.
    • Journal Title

      J. Reprod. Dev.

      Volume: 58 Pages: 260-263

    • NAID

      10030754171

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers.2012

    • Author(s)
      Abdel-Ghani, M., Shimizu, T., Asano, T. and Suzuki, H.
    • Journal Title

      Theriogenology

      Volume: 77 Pages: 347-355

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos and resulting pregnancies.2012

    • Author(s)
      Suzuki, H.
    • Journal Title

      Reprod. Dom. Anim.

      Volume: 47 Pages: 141-143

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sexing of dog sperm by fluorescene in situ hybridization.2012

    • Author(s)
      Oi, M., Yamada, K., Hayakawa, H. and Suzuki, H.
    • Journal Title

      J. Reprod. Dev.

      Volume: 59 Pages: 92-96

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of bovine cumulus-oocyte complexes-conditioned medium on in vitro maturation of canine oocytes. Reprod2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y., Asano, T., Hamano, S., Suzuki, H.
    • Journal Title

      Med. Biol

      Volume: 10 Pages: 43-49

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos2011

    • Author(s)
      Abe, Y., Suwa, Y., Asano, T., Ueta, Y. Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K., Suzuki, H.
    • Journal Title

      Biol. Reprod

      Volume: 84 Pages: 363-368

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A study on freeze-drying as a method of preserving mouse sperm.2011

    • Author(s)
      Kawase, Y., Suzuki, H.
    • Journal Title

      J. Reprod. Dev

      Volume: 57 Pages: 176-182

    • NAID

      10028274982

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y., Asano, T., Suzuki, H.
    • Journal Title

      Reprod. Med. Biol

      Volume: 10 Pages: 259-266

    • NAID

      10030444416

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of intraperitoneal administration of desialylated erythropoietin on the follicular survival in cryopreserved canine ovaries after xenotransplantation2011

    • Author(s)
      Hirayama, Y., Inoue, K., Suzuki, H.
    • Journal Title

      J.Mamm. Ova Res

      Volume: 28 Pages: 143-147

    • NAID

      10029898554

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of bovine cumulus-oocyte complexes-conditioned medium on in-vitro maturation of canine oocytes.2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y., Asano, T., Hamano, S. and Suzuki, H.
    • Journal Title

      Reprod. Med. Biol.

      Volume: 10 Pages: 43-49

    • NAID

      10029042897

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos.2011

    • Author(s)
      Abe, Y., Suwa, Y., Asano, T., Ueta, Y. Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K. and Suzuki, H.
    • Journal Title

      Biol. Reprod.

      Volume: 84 Pages: 363-368

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A study on freeze-drying as a method of preserving mouse sperm.2011

    • Author(s)
      Kawase, Y. and Suzuki, H.
    • Journal Title

      J. Reprod. Dev.

      Volume: 57 Pages: 176-182

    • NAID

      10028274982

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice.2011

    • Author(s)
      Abdel-Ghani, M., Abe, Y., Asano, T. and Suzuki, H.
    • Journal Title

      Reprod. Med. Biol.

      Volume: 10 Pages: 259-266

    • NAID

      10030444416

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of intraperitoneal administration of desialylated erythropoietin on the follicular survival in cryopreserved canine ovaries after xenotransplantation.2011

    • Author(s)
      Hirayama, Y., Inoue, K. and Suzuki, H.
    • Journal Title

      J. Mamm. Ova Res.

      Volume: 28 Pages: 143-147

    • NAID

      10029898554

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 蛍光in situハイブリダイゼーション(FISH)によるイヌの性染色体異数性精子の検出2013

    • Author(s)
      小牧春菜、大井真矢、鈴木宏志
    • Organizer
      第155回日本獣医学会学術集会
    • Place of Presentation
      東京大学駒場、東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Puppies from liquid nitrogen -Cryopreservation of canine embryos-2012

    • Author(s)
      Suzuki, H.
    • Organizer
      7th International Symposium on Canine and Feline Reproduction
    • Place of Presentation
      Whistler, British Columbia, Canada
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Effect of hyperbaric oxgen therapy on the follicular reserve on canine ovarian tissues after xenotransplantation2012

    • Author(s)
      Suzuki, H.
    • Organizer
      17th International Congress on Animal Reproduction
    • Place of Presentation
      Vancouver, British Columbia, Canada.
    • Related Report
      2012 Final Research Report
  • [Presentation] マラリア原虫感染が妊娠時特異的に重篤化する免疫学的メカニズムの解明2012

    • Author(s)
      新田あかね、白砂孔明、松本茜、鈴木宏志
    • Organizer
      第105回日本繁殖生物学会
    • Place of Presentation
      筑波大学会館、つくば市
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of the immunological mechanisms that malaria leads to a pregnancy-specific severity2012

    • Author(s)
      Nitta, A., Shirasuna, K., Matsumoto, A., Suzuki, H.
    • Organizer
      45th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      State College, Pennsylvania, USA.
    • Related Report
      2012 Final Research Report
  • [Presentation] Sperm Sexing in the dog by fluorescence in situ hybridization2012

    • Author(s)
      Suzuki, H., Oi, M.
    • Organizer
      45th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      State College, Pennsylvania, USA.
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of hyperbaric oxygen therapy on the follicular reserve of canine ovarian tissues after xenotransplantation.2012

    • Author(s)
      Suzuki, H.
    • Organizer
      17th International Congress on Animal Reproduction.
    • Place of Presentation
      Vancouver, British Columbia, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] マラリア原虫感染が妊娠時特異的に重篤化する免疫学的メカニズムの解明2012

    • Author(s)
      新田あかね、白砂孔明、松本茜、鈴木宏志
    • Organizer
      第105回日本繁殖生物学会大会
    • Place of Presentation
      筑波大学会館、つくば
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of the immunological mechanisms that malaria leads to a pregnancy-specific severity.2012

    • Author(s)
      Nitta, A., Shirasuna, K., Matsumoto, A., Suzuki, H.
    • Organizer
      SSR 45th Annual Meeting
    • Place of Presentation
      State College, Pennsylvania, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Sperm sexing in the dog by fluorescence in sity hybridization2012

    • Author(s)
      Suzuki, H., Oi, M.
    • Organizer
      SSR 45th Annual Meeting
    • Place of Presentation
      State College, Pennsylvania, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of hyperbaric oxygen therapy on the follicular loss of ovarian tissues after transplantation2011

    • Author(s)
      Suzuki, H., Hayashi, H., Asano, T.
    • Organizer
      44th Annual Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      Portland, Oregon, USA.
    • Related Report
      2012 Final Research Report
  • [Presentation] Cryopreservation of canine embryos2011

    • Author(s)
      Suzuki, H.
    • Organizer
      The 12th International Congress on Reproductive Biomedicine
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran
    • Related Report
      2012 Final Research Report
  • [Presentation] Follicular loss of cryopreserved canine ovary after xenotransplantation and its solution2011

    • Author(s)
      Suzuki, H.
    • Organizer
      The 12th International Congress on Reproductive Biomedicine
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran
    • Related Report
      2012 Final Research Report
  • [Presentation] Puppies from frozen embryos2011

    • Author(s)
      Suzuki, H.
    • Organizer
      International Meeting for Evolution of Reproductive Biology and Task of Frontiers: Trajectory and Prospective of IVF, Stem Cell and Epigenetic Study
    • Place of Presentation
      AIINA Hall, Morioka, Iwate, Japan.
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of hyperbaric oxygen therapy on the follicular loss of ovarian tissues after transplantation.2011

    • Author(s)
      Suzuki, H., Hayashi, S. and Asano, T.
    • Organizer
      44th Annual Meeting of the Society for the Study of Reproduction.
    • Place of Presentation
      Portland, Oregon, USA.
    • Related Report
      2011 Research-status Report
  • [Presentation] Cryopreservation of canine embryos.2011

    • Author(s)
      Suzuki, H.
    • Organizer
      the 12th International Congress on Reproductive Biomedicine(招待講演)
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran
    • Related Report
      2011 Research-status Report
  • [Presentation] Follicular loss of cryopreserved canine ovary after xenotransplantation and its solution.2011

    • Author(s)
      Suzuki, H.
    • Organizer
      the 12th International Congress on Reproductive Biomedicine(招待講演)
    • Place of Presentation
      Royan Institute, Tehran, The Islamic Republic of Iran.
    • Related Report
      2011 Research-status Report
  • [Presentation] Puppies from frozen embryos.2011

    • Author(s)
      Suzuki, H.
    • Organizer
      International Meeting for Evolution of Reproductive Biology and Task of Frontiers: Trajectory and Prospective of IVF, Stem Cell and Epigenetic Study.(招待講演)
    • Place of Presentation
      AIINA Hall, Morioka, Iwate.
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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