The cell determination to extraembryonic tissue and the human placental stem cell.

• Project/Area Number: 23659768
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: ARIMA Takahiro 東北大学, 大学院・医学系研究科, 教授 (80253532)
• Co-Investigator(Kenkyū-buntansha): HIURA Hitoshi 東北大学, 大学院・医学系研究科, 助教 (70451523) ; OKAE Hiroaki 東北大学, 大学院・医学系研究科, 助教 (10582695)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ヒト胎盤幹細胞 / エピジェネティックス / 細胞分化 / ゲノムインプリンティング / 胎盤発生 / TS細胞 / Cdx2 / Oct3/4

Research Abstract

The first cell differentiation in the mammalian development separates the trophoblast and embryonic cell lineages, resulting in the formation of the trophectoderm (TE) and inner cell mass (ICM) in blastocysts. Cdx2 gene expressed strongly in TE and Oct3/4 expressed in ICR. In this study, we determined the epigenetic mechanism of Cdx2 gene expression using trophoblastic stem cell (TS cell).

Research Products

• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproductive technologies. (2012)
  • Author(s): Hiura H, Okae H, Miyauchi N, Sato F, Sato A , Van De Pette M, John R M, Kagami M, Nakai K, Soejima H, Ogata T, Arima T.
  • Journal Title: Human Reproduction Volume: 27(8) Issue: 8 Pages: 2541-2548
  • DOI: 10.1093/humrep/des197
  • Peer Reviewed
• [Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method. (2012)
  • Author(s): Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T.
  • Journal Title: BMC Medical Genomics Volume: 5 Issue: 1 Pages: 8-17
  • DOI: 10.1186/1755-8794-5-8
  • Peer Reviewed
• [Journal Article] Distribution and Y397 phosphorylation of focal adhesion kinase on follicular development in the mouse ovary (2012)
  • Author(s): Sakurai M, Ohtake J, Ishikawa T, Tanemura K, Hoshino Y, Arima I, Sato E
  • Journal Title: Cell and Tissue Volume: 347 Issue: 2 Pages: 457-465
  • DOI: 10.1007/s00441-011-1307-2
  • Peer Reviewed
• [Journal Article] Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression (2012)
  • Author(s): Hiroaki Okae, et al
  • Journal Title: Human Molecular Genetics Volume: 21 Issue: 3 Pages: 548-558
  • DOI: 10.1093/hmg/ddr488
  • Peer Reviewed
• [Journal Article] JapaneseJournal of Reproductive Endocrinology「生殖補助医療由来の先天性ゲノムイン プリンティング異常症」 (2012)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明
  • Journal Title: 日本生殖内分泌学会雑誌 Volume: 17 Pages: 54-58
• [Journal Article] 生殖補助医療由来の先天性ゲノムインプリンティング異常症 (2012)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明
  • Journal Title: 日本生殖内分泌学会雑誌 Japanese Journal of Reproductive Endocrinology Volume: 17 Pages: 54-58
  • NAID: 40019434316
• [Journal Article] Aberrant DNA Methylation of Imprinted Loci (2012)
  • Author(s): Arima T, Okae H, Hiura H, Hayashi C et al
  • Journal Title: INTECH Volume: 29 Pages: 183-192
  • Peer Reviewed
• [Journal Article] The physical ability of Japanese female elderly with cerebrovascular disease correlates to the telomere length and subtelomeric methylation status in their peripheral blood leukocytes (2011)
  • Author(s): Maeda T, Oyama J, Higuchi Y, Nishiyama Y, Kudo Y, Yamori Y, Nakazono T, Arima I, Mimori K, Makino N
  • Journal Title: Gerontology Volume: 57 Issue: 2 Pages: 137-143
  • DOI: 10.1159/000314633
  • Peer Reviewed
• [Journal Article] The correlation between the clinical laboratory data and the telomere length in peripheral blood leukocytes of Japanese female patients with hypertension. The Journal of Nutrition (2011)
  • Author(s): Maeda T, Oyama J, Sasaki M, Arima T, Makino N
  • Journal Title: Health and Aging (JNHA) Volume: 15 Pages: 240-244
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21369674
  • Peer Reviewed
• [Journal Article] Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis (2011)
  • Author(s): Sato A, Hiura H, Okae H, Miyauchi N, Abe Y, Utsunomiya T, Yaegashi N, Arima T
  • Journal Title: Fertil and Steril Volume: 95 Pages: 129-134
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
  • Journal Title: Science Volume: 332 Issue: 6031 Pages: 848-52
  • DOI: 10.1126/science.1203919
  • Peer Reviewed
• [Journal Article] ゲノムインプリンティングと発がん癌と化学療法 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、宮内尚子、佐藤芙美
  • Journal Title: 癌と化学療法社 Pages: 1745-1749
  • URL: http://www.pieronline.jp/content/article/0385-0684/38110/1745
• [Journal Article] 母子の健康と環境影響助産雑誌 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Journal Title: 医学書院 Volume: 62 Pages: 11-11
  • URL: http://www.medbook.jp/eshopdo/refer/refer.php?sid=ns13533&cid=37&scid=211&mcid=&me=&vmode=&view_id=665-65-11&PHPSESSID=95fa583906e9fda3b226b39bc36e7518
• [Journal Article] ARTにおけるエピジェティクス異常産婦人科の実際 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子、阿部千鶴、林千賀
  • Journal Title: 金原出版株式会社 Pages: 741-750
  • URL: http://search.jamas.or.jp/link/ui/2011191627
• [Journal Article] Assessing loss of imprint methylation in sperm from subfertile men using novel methylation PCR-Luminex analysis (2011)
  • Author(s): Sato A, Hiura H, Okae H, Miyauchi N, Abe Y, Utsunomiya T, Yaegashi N, Arima T
  • Journal Title: Fertil and Steril Volume: 95 Issue: 1 Pages: 129-134
  • DOI: 10.1016/j.fertnstert.2010.06.076
  • Peer Reviewed
• [Journal Article] The correlation between the clinical laboratory (2011)
  • Author(s): Maeda T, Oyama J, Sasaki M, Arima T, Makino N.
  • Journal Title: The Journal of Nutrition, Health and Aging (JNHA) Volume: 15 Pages: 240-244
  • Peer Reviewed
• [Presentation] 乏精子症とゲノムインプリンティング (2013)
  • Author(s): 有馬隆博
  • Organizer: 日本生殖再生医学会・第8回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-10
• [Presentation] 生殖医療とエピジェネティクス (2012)
  • Author(s): 有馬隆博
  • Organizer: 日本不妊カウンセリング学会・第11回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2012-06-08
• [Presentation] ARTにおけるエピジェネティク機構 (2012)
  • Author(s): 有馬隆博
  • Organizer: 日本生殖再生医学会・第7回学術集会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-25
• [Presentation] ART and Epigenetic Errors - Abnormal DNA methylation in imprinting disorders after ART (2012)
  • Author(s): Arima T
  • Organizer: The International Ovarian Conference 2012
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-03-17
• [Presentation] インプリント遺伝子の分子間相互機構の解明 (2012)
  • Author(s): 有馬 隆博
  • Organizer: 熊本大学セミナー(招待講演)
  • Place of Presentation: 熊本大学
• [Presentation] ART and Epigenetic Errors - Abnormal (2012)
  • Author(s): 有馬 隆博
  • Organizer: 卵巣に関する国際カンファレンス2012(招待講演)
  • Place of Presentation: 東京
• [Presentation] ARTにおけるエピジェネティク機構 (2012)
  • Author(s): 有馬 隆博
  • Organizer: 日本生殖再生医学会・第7回学術集会(招待講演)
  • Place of Presentation: 東京
• [Presentation] ｹﾞﾉﾑｲﾝﾌﾟﾘﾝﾃｨﾝｸﾞとヒト疾患 (2011)
  • Author(s): 有馬 隆博
  • Organizer: 第96回東北医学会総会(招待講演)
  • Place of Presentation: 仙台
• [Presentation] 胎盤特異的インプリント遺伝子の役割 (2011)
  • Author(s): 有馬 隆博
  • Organizer: 第4回生殖系列の世代サイクルとエピゲノムネットワーク(招待講演)
  • Place of Presentation: 大阪
• [Book] 遺伝子医学MOOK25号 (2013)
  • Author(s): 千葉初音、岡江寛明、有馬隆博
  • Publisher: 株式会社メディカルドゥ
• [Book] 遺伝子医学MOOK 25号 (2013)
  • Author(s): 千葉初音、岡江寛明、有馬隆博
  • Publisher: 株式会社メディカルドゥ
• [Book] Aberrant DNA methylation of imprinted loci in male and female germ cells of infertilecouples (2012)
  • Author(s): Arima T, Okae H, Hiura H, Miyauchi N, Sato F, Sato A, Hayashi C
  • Publisher: INTECH. DNA METHYLATION
• [Book] DNA METHYLATION (2012)
  • Author(s): Arima T, Okae H, Hiura H, Miyauchi N, Sato F, Sato A, Hayashi C.
  • Publisher: INTECH
• [Book] 卵子学 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Publisher: 京都大学学術出版会
• [Book] 産婦人科の実際 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子、阿部千鶴、林千賀
  • Publisher: 金原出版株式会社
• [Book] 助産雑誌 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子、宮内尚子
  • Publisher: 医学書院
• [Book] 癌と化学療法 (2011)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、宮内尚子、佐藤芙美
  • Publisher: 癌と化学療法社
• [Patent(Industrial Property Rights)] ヒト精子の質的機能評価システムに応 用する DNA メチル化解析システムの開発 (2012)
  • Inventor(s): 有馬 隆博
  • Industrial Property Rights Holder: 東北大学
  • Filing Date: 2012-05-16
  • Overseas
• [Patent(Industrial Property Rights)] ヒト精子の質的機能評価システムに応用するDNAメチル化解析システムの開発 (2012)
  • Inventor(s): 有馬隆博
  • Industrial Property Rights Holder: 東北大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-05-16
  • Overseas