The investigation for molecular mechanisms and the prevention of preterm birth
| Project/Area Number |
23659771
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Gunma University |
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Principal Investigator |
KIGURE KEIKO 群馬大学, 医学部附属病院, 助教 (00598609)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | cAMP / AM / LH-R / 子宮収縮 / 炎症 |
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| Research Abstract |
In the present study, we examined the effect of IL-6 and TNF-alpha on the expression of LH-R mRNA and protein expression levels and the mechanisms of regulation in rat primary granulosa cells and human amniotic epithelial cells. TNF-alpha stimulated the expression of IL-6 and negatively regulated LH-R mRNA. TNF-alpha enhanced acutivin A production in time- and dose-dependent manner in amniotic epithelial cells.
We have shown that IL-6 up-regulates LHR due to increase transcription level of mRNA, and JAK/STAT3 signaling is required for this up-regulation by IL-6. In terms of TNF-alpha, we speculate that TNF-alpha enhanced the transportation of NF-kappaB from cytosol into nucleus.
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Report
(4 results)
Research Products
(12 results)
