Induction of tissue remodeling of reproductive organs using gligl stem cell-derived factors
| Project/Area Number |
23659778
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NISIO Takeshi 京都大学, 医学研究科, 助教 (70303790)
SATO Yukiyasu 京都大学, 医学研究科, 助教 (00508236)
ARAKI Yosihiko 順天堂大学, 医学研究科, 准教授 (70250933)
FUJIWARA Tomoko 芦屋学園短期大学, 生活創造学科, 教授 (60310744)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | グリア細胞 / 組織再構築 / 生殖臓器 / 幹細胞 / 上皮細胞 |
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| Research Abstract |
Co-Investigator Nishio found that when stem cells of the neural epithelium-derived glia cells were transplanted into the severed site of the cordotomized mature rat, a rapid and successful regeneration of neuronal axons beyond the lesion site was achieved during several days. Central neural tissues construct tree-dimensional structures, creating complicated neural networks, which are composed of neuroepithelium-derived neurons and glial cells. In this respect, central nervous system has been far evolved than ordinary 2-dimensional epithelial layer and therefore it is speculated that the role of the glial cells in reconstruction will throw a new insight to the process of the reconstruction in reproductive organs. Based on this concept, this study was aimed to elucidate new mechanisms of developing and/or reconstructive processes of granulosa cells, endometrial epithelial cell and trophoblasts that belong to epithelial cell-lineages. First, we screened the effects of glial cell-derived factors on the culture of granulosa cells, endometrial epithelial cells, and trophoblasts. As a result, human telomerase reverse transcriptase(h-TERT)-transfected humam endometrial epithelial cell line(provided from Dr. Kyo at Kanazawa University) promoted its intercellular connection in the presence of glial stem cells derived from EGFP-transgenic rats, showing the possibility that immature glia cell-derived soluble factors can be one of the candidates to induce reconstruction of reproductive organs and to become a useful tool in the field of reproductive medicine.
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Report
(2 results)
Research Products
(13 results)
