Humanin-mediated rescue of testicular germ cell apoptosis
| Project/Area Number |
23659784
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ヒューマニン / 男性不妊 / 造精細胞死 |
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| Research Abstract |
One of the central mechanism underlying male infertility has been assumed to be the abnormal increase in testicular germ cell apoptosis. However, its detailed mechanism remains unknown and the development of therapy inhibiting testicular germ cell apoptosis has not been developed. In this study, to address the question as to whether and how the Humanin/EH-mediated signal is involved in inhibition of germ cell apoptosis, we have first succeeded in developing a mouse assay system by which the administration of an LHRH-antagonist Cetrorelix induces apoptosis in mouse testicular germ cells in vivo. Second, we have found that EH plays a major role in vivo instead of Humanin and succeeded in purifying a large amount of recombinant EH in bacteria.
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Report
(3 results)
Research Products
(6 results)
