| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Research Abstract |
The aim of this study was to evaluate the difference of micro RNA (miRNA) expression profiles between in lungs of the autopsied patients with amniotic fluid embolism and in those with non-amniotic fluid embolism. We used formalin fixed paraffin embedded lung tissues of the three autopsied patients with amniotic fluid embolism and one autopsied patient with non-amniotic fluid embolism as a control. RNA was extracted from formalin fixed paraffin embedded lung tissues. The qualities of extracted RNA were evaluated by using A260(nucleic acid)/A280(protein) ratio. Then, miRNA expression profiles were analysed by Toray miRNA Oligo Chip. In lung tissues of the patients with amniotic fluid embolism, intensities of five miRNAs increased more than 1 1/2 times than in those with non-amniotic fluid embolism. Contrary,in lung tissues of the patients with amniotic fluid embolism, intensities of12 miRNAs decreased more than 1 1/2 times than in those with non-amniotic fluid embolism.
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