Micro RNA expression profile in lungs of the autopsied patients with amniotic fluid embolism

• Project/Area Number: 23659786
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: YONEYAMA Koichi 日本医科大学, 医学部, 講師 (90220772)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 羊水塞栓症 / マイクロRNA / マイクロＲＮＡ / ＦＦＰＥ

Research Abstract

The aim of this study was to evaluate the difference of micro RNA (miRNA) expression profiles between in lungs of the autopsied patients with amniotic fluid embolism and in those with non-amniotic fluid embolism. We used formalin fixed paraffin embedded lung tissues of the three autopsied patients with amniotic fluid embolism and one autopsied patient with non-amniotic fluid embolism as a control. RNA was extracted from formalin fixed paraffin embedded lung tissues. The qualities of extracted RNA were evaluated by using A260(nucleic acid)/A280(protein) ratio. Then, miRNA expression profiles were analysed by Toray miRNA Oligo Chip. In lung tissues of the patients with amniotic fluid embolism, intensities of five miRNAs increased more than 1 1/2 times than in those with non-amniotic fluid embolism. Contrary,in lung tissues of the patients with amniotic fluid embolism, intensities of12 miRNAs decreased more than 1 1/2 times than in those with non-amniotic fluid embolism.

Research Products

• [Journal Article] Clinical characteristics of amniotic fluid embolism : An experience of 29 years (2014)
  • Author(s): Koichi Yoneyama, Atsuko Sekiguchi, Takashi Matsushima, Rieko Kawase, Akihito Nakai, Hirobumi Asakura and Toshiyuki Takeshita
  • Journal Title: J Obstet and Gynaecol Res Volume: (in Press)
  • Peer Reviewed
• [Journal Article] Clinical characteristics of amniotic fluid embolism: An experience of 29 years (2014)
  • Author(s): Koichi Yoneyama, Atsuko Sekiguchi, Takashi Matsushima, Rieko Kawase, Akihito Nakai, Hirobumi Asakura and Toshiyuki Takeshita
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 未定
  • Peer Reviewed
• [Presentation] 発症時期に視点をおいた羊水塞栓症の臨床的特徴 (2014)
  • Author(s): 米山剛一、関口敦子、松島 隆、川瀬里衣子、中井章人、朝倉啓文、竹下俊行
  • Organizer: 第66回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
• [Presentation] 発症時期に視点を置いた羊水塞栓症の臨床的特徴 (2014)
  • Author(s): 米山剛一、関口敦子、松島 隆、川瀬里衣子、中井章人、朝倉啓文、竹下俊行
  • Organizer: 第66回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 頸管縫縮糸抜糸は羊水塞栓症の危険因子となり得るか (2012)
  • Author(s): 米澤美令、米山剛一、松本二郎、白銀 恵、中西一歩、村川裕子、高屋 茜、山田 隆、渡辺美千明、中井章人、朝倉啓文、竹下俊行
  • Organizer: 第123回関東連合産科婦人科学会
  • Place of Presentation: 東京
  • Year and Date: 2012-06-17
• [Presentation] 頸管縫縮糸抜糸後に発症した致死的羊水塞栓症2症例の検討 (2012)
  • Author(s): 米山剛一、米澤美令、高屋 茜、永野玲子、伊藤麻利江、中井章人、朝倉啓文、竹下俊行
  • Organizer: 第48回日本周産期・新生児学会
  • Place of Presentation: 埼玉、大宮
• [Presentation] 頸管縫縮糸抜糸後に発症した致死的羊水塞栓症2症例の検討 (2012)
  • Author(s): 米山 剛一, 米澤 美令, 高屋 茜, 永野玲子、伊藤麻利江、中井 章人, 朝倉 啓文, 竹下 俊行
  • Organizer: 第48回日本周産期・新生児医学会
  • Place of Presentation: 埼玉、大宮
• [Presentation] 頸管縫縮糸抜糸は羊水塞栓症発症の危険因子となり得るか
  • Author(s): 米澤 美令, 米山 剛一, 松本 二朗, 白銀 恵, 中西 一歩, 村川 裕子, 高屋 茜, 山田 隆, 渡辺 美千明, 中井 章人, 朝倉 啓文, 竹下 俊行
  • Organizer: 第123回関東連合産科婦人科学会
  • Place of Presentation: 東京