• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The physiological role of CD9 and exosomes in oviducts and uteri

Research Project

Project/Area Number 23659788
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionNational Research Institute for Child Health and Development

Principal Investigator

MIYADO KENJI  独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 室長 (60324844)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords不妊 / 出産 / 子宮内膜上皮 / 再生 / CD9 / VEGF-A / エキソソーム / 子宮内膜 / 子宮 / VEGF
Research Abstract

In mammals, the female reproductive tract is remodeled cyclically throughout adult life. Despite the expression of CD9 in the epithelium of the uterus, its role is unclear. Here, we addressed this issue by examining fertilization-competent Cd9-/- mice expressing CD9-GFP in their eggs (Cd9-/-TG). Immunobiochemical analysis demonstrated that CD9 was present in the uterine secretions. Electron microscopic analysis revealed the extracellular matrix-like feature of CD9-containing structures. We also found that the litter size of Cd9-/-TG female mice was significantly reduced after their first birth. Histological analysis revealed severely delayed re-epithelialization of the endometrium both in vitro and in vivo in mice. The quantity of vascular endothelial growth factor-A (VEGF-A) was remarkably reduced in Cd9-/-TG female mice. These results provide the first evidence that CD9-mediated VEGF secretion plays a role in re-epithelialization of the uterus.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (34 results)

All 2014 2013 2012 2011 Other

All Journal Article (19 results) (of which Peer Reviewed: 16 results,  Open Access: 3 results) Presentation (9 results) (of which Invited: 3 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Absence of CD9 reduces endometrial VEGF secretion and impairs uterine repair after parturition2014

    • Author(s)
      N Kawano, K Miyado, N Yoshii, S Kanai, H Saito, M Miyado, N Inagaki, Y Odawara, T Hamatani, A Umezawa
    • Journal Title

      Scientific reports

      Volume: 4 Issue: 1 Pages: 4701-4701

    • DOI

      10.1038/srep04701

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Derivation of human decidua-like cells from amnion and menstrual blood2014

    • Author(s)
      Sugawara K, Hamatani T, Yamada M, Ogawa S, Kamijo S, Kuji N, Akutsu H, Miyado K, Yoshimura Y, Umezawa A
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 4599-4599

    • DOI

      10.1038/srep04599

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Seminal vesicle protein SVS2 is required for sperm survival in the uterus2014

    • Author(s)
      N Kawano, N Araki, K Ybshida, THibino, N Ohnami, M Makino, S Kanai, H Hasuwa, M Yoshida, K Miyado, A Umezawa
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 111(11) Issue: 11 Pages: 4145-4150

    • DOI

      10.1073/pnas.1320715111

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Age-associated telomere shortening in mouse oocytes2013

    • Author(s)
      Yamada-Fukunaga T, Yamada M, Hamatani T, Chikazawa N, Ogawa S, Akutsu H, Miura T, Miyado K, Tarin JJ, Kuji N, Umezawa A, Yoshimura Y
    • Journal Title

      Reprod Biol Endocrinol

      Volume: 11 Issue: 1 Pages: 108-108

    • DOI

      10.1186/1477-7827-11-108

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] β-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells2013

    • Author(s)
      Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, Yoshida K, Ichida JK, Yamada M, Hamatani T, Kuji N, Miyado K, Yoshimura Y, Umezawa A
    • Journal Title

      PLoS One

      Volume: 8(5) Issue: 5 Pages: 63265-63265

    • DOI

      10.1371/journal.pone.0063265

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integration of the mouse sperm fertilization-related protein equatorin into the acrosome during spermatogenesis as revealed by super-resolution and immunoelectron microscopy2013

    • Author(s)
      Ito C, Yamatoya K, Yoshida K, Fujimura L, Hatano M, Miyado K, Toshimori K
    • Journal Title

      Cell Tissue Res

      Volume: 352(3) Issue: 3 Pages: 739-750

    • DOI

      10.1007/s00441-013-1605-y

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] テトラスパニンによる受精の膜融合制御2013

    • Author(s)
      宮戸健二
    • Journal Title

      医学のあゆみ

      Volume: Vol.244, No.2 Pages: 181-181

    • Related Report
      2013 Final Research Report
  • [Journal Article] Critical role of exosomes in sperm-egg fusion and virus-induced cell-cell fusion2013

    • Author(s)
      Harada Y, Yoshida K, Kawano N, Miyado K
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 1 Pages: 1-1

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Beta-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells2013

    • Author(s)
      Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, Yoshida K, Yamada M, Hamatani T, Miyado K, Yoshimura Y, Umezawa A
    • Journal Title

      PLOS ONE

      Volume: 1 Pages: 1-1

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] CD81 and CD9 work independently as extracellular components upon fusion of sperm and oocyt2012

    • Author(s)
      Ohnami N, Nakamura A, Miyado M, Sato M, Kawano N, Yoshida K, Harada Y, Takezawa Y, Kanai S, Ono C, Takahashi Y, Kimura K, Shida T, Miyado K, Umezawa A
    • Journal Title

      Biol Open

      Volume: 1(7) Issue: 7 Pages: 640-647

    • DOI

      10.1242/bio.20121420

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mamld1 deficiency significantly reduces mRNA expression levels of multiple genes expressed in mouse fetal Leydig cells but permits normal genital and reproductive development2012

    • Author(s)
      Miyado M, Nakamura M, Miyado K, Morohashi K, Sano S, Nagata E, Fukami M, Ogata T
    • Journal Title

      Endocrinology

      Volume: 153(12) Issue: 12 Pages: 6033-6040

    • DOI

      10.1210/en.2012-1324

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Impaired fertilizing ability of Sodl-deficient mouse sperm during in-vitro fertilization.2012

    • Author(s)
      Tsunoda Satoshi, Kawano Natsuko, Miyado Kenji, Kim ura Naoko, Fujii Junichi.
    • Journal Title

      Biology of reproduction

      Volume: 87 Issue: 5 Pages: 1-6

    • DOI

      10.1095/biolreprod.112.102129

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 卵由来エキソソームによる細胞融合の促進機構2012

    • Author(s)
      宮戸健二
    • Journal Title

      医学のあゆみ

      Volume: Vol.241,No.11 Pages: 861-862

    • Related Report
      2013 Final Research Report
  • [Journal Article] CD9 is critical for cutaneous wound healing through JNK signaling.2012

    • Author(s)
      Zhang J, Dong J, Gu H, Yu S, Zhang X, Gou Y, Xu W, Burd A, Huang L, Miyado K, Huang Y, Chan HC.
    • Journal Title

      Journal of Investigative Dermatology

      Volume: 132 Issue: 1 Pages: 226-236

    • DOI

      10.1038/jid.2011.268

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion2011

    • Author(s)
      Takezawa Y, Yoshida K, Miyado K, Sato M, Nakamura A, Kawano N, Sakakibara K, Kondo T, Harada Y, Ohnami N, Kanai S, Miyado M, Saito H, Takahashi Y, Akutsu H, Umezawa A
    • Journal Title

      Sci Rep

      Volume: 1 Issue: 1 Pages: 68-68

    • DOI

      10.1038/srep00068

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] GSTT1 is upregulated by oxidative stress through p38-MK2 signaling pathway in human granulosa cells : possible association with mitochondrial activity2011

    • Author(s)
      Ito M, Imai M, Muraki M, Miyado K, Qin J, Kyuwa S, Yoshikawa Y, Hosoi Y, Saito H, Takahashi Y
    • Journal Title

      Aging (Albany NY)

      Volume: 3(12) Pages: 1213-23

    • Related Report
      2013 Final Research Report
  • [Journal Article] Lipid rafts : keys to sperm maturation, fertilization, and early embryogenesis2011

    • Author(s)
      Kawano N, Yoshida K, Miyado K, Yoshida M
    • Journal Title

      J Lipids

      Volume: 2011 Pages: 264706-264706

    • DOI

      10.1155/2011/264706

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage2011

    • Author(s)
      Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N
    • Journal Title

      BMC Dev Biol

      Volume: 11 Issue: 1 Pages: 22-22

    • DOI

      10.1186/1471-213x-11-22

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] GSTT1 is upregulated by oxidative stress through p38-MK2 signaling pathway in human granulosa cells: possible association with mitochondrial activity.2011

    • Author(s)
      Ito M, Imai M, Muraki M, Miyado K, Qin J, Kyuwa S, Yoshikawa Y, Hosoi Y, Saito H, Takahashi Y.
    • Journal Title

      Aging

      Volume: 3 Pages: 1213-1223

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] テトラスパニンを介した細胞融合の分子メカニズム、ワークショップ「4回膜貫通蛋白質の構造と機能解析の進展」2013

    • Author(s)
      宮戸健二
    • Organizer
      第36回日本分子生物学会年会
    • Related Report
      2013 Final Research Report
  • [Presentation] テトラスパニンを介した細胞融合の分子メカニズム2013

    • Author(s)
      宮戸健二
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 受精の膜融合におけるCD9およびエキソソームの役割、シンポジウム「バイオイメージングにより明らかにされた動・植物の有性生殖メカニズム」2012

    • Author(s)
      宮戸健二
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Related Report
      2013 Final Research Report
  • [Presentation] CD9 maintains uterine function through VEGF secretion from endometrial epithelium2012

    • Author(s)
      宮戸健二、河野菜摘子、吉井紀子、金井誠也、梅澤明弘、浜谷敏生
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] PLCzeta is involved in spermatogenesis in mice2012

    • Author(s)
      牧野麻亜子、伊藤昌彦、河野菜摘子、原田裕一郎、金井誠也、宮戸健二
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 哺乳類における受精の膜融合を制御する分子メカニズム2012

    • Author(s)
      宮戸健二
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] マウス卵の受精におけるクエン酸合成酵素による卵活性化の可能性2012

    • Author(s)
      原田裕一郎、吉田恵一、金井誠也、河野菜摘子、岩尾康宏、宮戸健二
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 受精のメカニズム:精子と卵の膜融合を制御する分子機構2012

    • Author(s)
      宮戸健二
    • Organizer
      日本受精着床学会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 精子と卵子の融合メカニズム:感染症と生殖をつなぐ構造体2011

    • Author(s)
      宮戸健二
    • Organizer
      第52回日本哺乳動物卵子学会
    • Place of Presentation
      国際医療福祉大学本校、大田原
    • Related Report
      2011 Research-status Report
  • [Book] Role of CD9 in sperm-egg fusion and virus-induced cell fusion in mammals. Sexual Reproduction in Animals and Plants, edited by Sawada H, Inoue N, Iwano M2014

    • Author(s)
      Yoshida K, Kawano N, Harada Y, Miyado K
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] 動植物の受精学 - 共通性と多様性 -2014

    • Author(s)
      澤田均, 編
    • Publisher
      化学同人
    • Related Report
      2013 Final Research Report
  • [Book] In Embryology- Updates and Highlights on Classic Topics2012

    • Author(s)
      Imai M, Qin J, Yamakawa N, Miyado K, Umezawa A, Takahashi Y
    • Total Pages
      200
    • Publisher
      InTech Open Publisher
    • Related Report
      2012 Research-status Report
  • [Book] In Embryology- Updates and Highlights on Classic Topics2012

    • Author(s)
      Imai M, Qin J, Yamakawa N, Miyado K, Umezawa A, Takahashi Y.
    • Total Pages
      20
    • Publisher
      InTech Open Publisher
    • Related Report
      2011 Research-status Report
  • [Book] Role of CD9 in sperm-egg fusion and its general role in fusion phenomena. Cell Fusions, edited by Lars-Inge Larsson2011

    • Author(s)
      Kawano N, Harada Y, Yoshida K, Miyado K
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.ncchd.go.jp/research/nch/reproduction/staff_miyado.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi