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Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device

Research Project

Project/Area Number 23659790
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionTohoku University

Principal Investigator

WADA Hiroshi  東北大学, 大学院・工学研究科, 教授 (30111264)

Co-Investigator(Kenkyū-buntansha) MURAKOSHI Michio  東北大学, 大学院・工学研究科, 助教 (70570901)
KOYAMA Shin  東北大学, 大学院・工学研究科, 助教 (10465487)
KOBAYSHI Toshimitsu  東北大学, 大学院・医学系研究科, 教授 (80133958)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords耳科学
Research Abstract

A membrane potential control device, which induces the conformational changes of prestin expressed in the plasma membrane, has been newly developed. Microstructures and electrophysiological properties of the prestin-expressing culture cells and the prestin-reconstituted lipid bilayer were measured by using this device. As a result, it was suggested that the conformational changes of proteins in the plasma membrane may possibly be visualized when the membrane potential is controlled using the developed device. Based on the findings obtained in this study, further research to visualize the conformational changes of prestin reconstituted in the lipid bilayer will be performed using this device.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2013 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] Piezoelectric materials mimic the function of the cochlear sensory epithelium2011

    • Author(s)
      Inaoka T et al.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Pages: 18390-18395

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Evaluation of electrophysiological properties of the motor protein prestin expressed in the inner ear2013

    • Author(s)
      Ogawa Y, Murakoshi M, Wada H
    • Organizer
      The 6th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      Singapore
    • Related Report
      2012 Final Research Report
  • [Presentation] 内耳に発 現する膜タンパク質プレスチンの電気 生理学的特性計測のための平面パッチ クランプシステムの開発(Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear)2013

    • Author(s)
      小川雄大,村越道生,和田仁
    • Organizer
      日本機械学会第25回バイ オエンジニアリング講演会
    • Place of Presentation
      筑波
    • Related Report
      2012 Final Research Report
  • [Presentation] 内耳に発現する膜タンパク質プレスチンの電気生理学的特性計測のための平面パッチクランプシステムの開発(Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear)2013

    • Author(s)
      小川雄大,村越道生,和田仁
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      つくば
    • Related Report
      2012 Annual Research Report
  • [Presentation] Evaluation of electrophysiological properties of the motor protein prestin expressed in the inner ear2013

    • Author(s)
      Ogawa Y, Murakoshi M, Wada H
    • Organizer
      The 6th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      シンガポール,シンガポール
    • Related Report
      2012 Annual Research Report
  • [Presentation] Prestin, the inner ear motor protein-its structure and function2012

    • Author(s)
      Wada H, Kumano S, Murakoshi M, Koyama S
    • Organizer
      The 18th Int. Symp. of Tohoku University GCOE
    • Place of Presentation
      仙台
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of membrane topology of prestin expressing in CHO cells2011

    • Author(s)
      Murakoshi M, Kawase T, Kumano S, Wada H
    • Organizer
      The 11th International Mechanics of Hearing WS
    • Place of Presentation
      ウィリアムズタウン,マサチューセッツ州,アメリカ
    • Related Report
      2011 Research-status Report
  • [Presentation] High-resolution AFM imaging of prestin purified and reconstituted into an artificial lipid bilayer2011

    • Author(s)
      Wada H, Kumano S, Murakoshi M, Iida K, Hamana H
    • Organizer
      The 11th International Mechanics of Hearing WS
    • Place of Presentation
      ウィリアムズタウン,マサチューセッツ州,アメリカ
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://db.tohoku.ac.jp/whois/detail/b6669433b8717e8ca8124a5ee024cc9f.html

    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.wadalab.mech.tohoku.ac.jp/index-j.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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