Induction of inner ear tissue by stem cell injection into the inner ear deficient mouse embryos
| Project/Area Number |
23659793
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takayuki 京都大学, 医学研究科, 講師 (50335270)
TAURA Akiko 京都大学, 医学研究科, 助教 (70515345)
YAMAMOTO Norio 京都大学, 医学研究科, 助教 (70378644)
SAKAMOTO Tatsunori 京都大学, 医学研究科, 助教 (60425626)
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| Research Collaborator |
NISHIMURA Koji 京都大学, 医学研究科, 大学院生
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 幹細胞 / 内耳 / 分化誘導 / 胚盤胞補完 / 再生 / キメラマウス / 内耳欠損マウス |
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| Research Abstract |
We verified the formation of a normal inner ear by blastocyst complementation. First, kidney formation by blastocyst complementation already have been reported in Sall1 knockout mice. From the work of Sall1 during development, the lack of the inner ear was expected in this Sall1 knockout mice. But normal inner ear formation were observed in the knockout mice. Then, we investigated the formation of a normal inner ear by blastocyst complementation in Pax2 or Six1 knockout mice. The deficient in the inner ear formation have been reported previously in these knockout mice. We obtained some inner ear formed mice from the GFP-positive ES cells injected into these knockout blastocysts. But wecouldn’t find the GFP-positive inner ear cells in those mice. As a result, we could not confirm the blastocyst complementation.
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Report
(3 results)
Research Products
(5 results)
