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Induction of inner ear tissue by stem cell injection into the inner ear deficient mouse embryos

Research Project

Project/Area Number 23659793
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionKyoto University

Principal Investigator

KITAJIRI Shin-ichiro  京都大学, 医学研究科, 助教 (00532970)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Takayuki  京都大学, 医学研究科, 講師 (50335270)
TAURA Akiko  京都大学, 医学研究科, 助教 (70515345)
YAMAMOTO Norio  京都大学, 医学研究科, 助教 (70378644)
SAKAMOTO Tatsunori  京都大学, 医学研究科, 助教 (60425626)
Research Collaborator NISHIMURA Koji  京都大学, 医学研究科, 大学院生
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords幹細胞 / 内耳 / 分化誘導 / 胚盤胞補完 / 再生 / キメラマウス / 内耳欠損マウス
Research Abstract

We verified the formation of a normal inner ear by blastocyst complementation. First, kidney formation by blastocyst complementation already have been reported in Sall1 knockout mice. From the work of Sall1 during development, the lack of the inner ear was expected in this Sall1 knockout mice. But normal inner ear formation were observed in the knockout mice. Then, we investigated the formation of a normal inner ear by blastocyst complementation in Pax2 or Six1 knockout mice. The deficient in the inner ear formation have been reported previously in these knockout mice. We obtained some inner ear formed mice from the GFP-positive ES cells injected into these knockout blastocysts. But wecouldn’t find the GFP-positive inner ear cells in those mice. As a result, we could not confirm the blastocyst complementation.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2013 2012 2011

All Journal Article (2 results) Presentation (1 results) Book (2 results)

  • [Journal Article] Fates of murine pluripotent stem cell-derived neural progenitors following transplantation into mouse cochleae2012

    • Author(s)
      Nishimura K, Nakagawa T, Sakamoto T, Ito J
    • Journal Title

      Cell Transplant

      Volume: 21 Pages: 763-771

    • NAID

      120004057181

    • Related Report
      2012 Final Research Report
  • [Journal Article] Adipose tissue-derived stromal cells protect hair cells from aminoglycoside2011

    • Author(s)
      Yoshida A, Kitajiri S, Nakagawa T, Hashido K, Inaoka T, Ito J
    • Journal Title

      Laryngoscope

      Volume: 121 Pages: 1281-1286

    • Related Report
      2012 Final Research Report
  • [Presentation] 内耳再生医療開発と未来の難聴治療2013

    • Author(s)
      中川隆之
    • Organizer
      第68回山形県耳鼻咽喉科疾患研究会
    • Place of Presentation
      山形
    • Year and Date
      2013-03-24
    • Related Report
      2012 Final Research Report
  • [Book] 遺伝子医学2012

    • Author(s)
      北尻真一郎
    • Total Pages
      6
    • Publisher
      メディカルドゥ
    • Related Report
      2012 Final Research Report
  • [Book] 遺伝子医学 MOOK222012

    • Author(s)
      北尻真一郎
    • Total Pages
      6
    • Publisher
      メディカルドゥ
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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