| Project/Area Number |
23659799
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
神谷 和作 順天堂大学, 医学部, 講師 (10374159)
飯塚 崇 順天堂大学, 医学部, 助教 (40372932)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMIYA Kazusaku 順天堂大学, 医学部, 講師 (10374159)
IIZUKA Takashi 順天堂大学, 医学部, 助教 (40372932)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 耳科学 / 再生医療 / 遺伝性難聴 / iPS細胞 / 内耳前駆細胞 / 幹細胞ホーミング / 多能性幹細胞 / 内耳有毛細胞 / 蝸牛線維細胞 |
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| Research Abstract |
In this study, we generated the novel inner ear progenitor cells from induced pluripotent stem (iPS) cells which express hair cell marker Myosin7a with proliferation potency. After the stimulation with FGF3 and FGF10, iPS cells were co-cultured with mitoticallyinactivated adult inner ear multipotent stem cells which we recently generated. They can be proliferate after the cryopreservation. In these methods, we obtained several lines of inner ear lineage cells such as hair cell marker Myosin7a positive cells with apical cilia composed of actin filament, Connexin26 expressing cells. It was suggested t hat these methods enables to replace multiple types of inner ear cells with any abnormalities
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