| Project/Area Number |
23659804
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Iwate University (2012)
Tohoku University (2011) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUGANO Eriko 岩手大学, 工学部, 特任准教授 (70375210)
伊藤 拓哉 東北大学, 医学(系)研究科(研究院), 助教 (70396539)
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 視覚再生 / 人工網膜 / チャネルロドプシン-2 / 遺伝子治療 / ウイルスベクター |
|---|
| Research Abstract |
The transduction efficiency to the cortex using AAV type 2/5 was higher than that using AAV2/2. However we could not succeed to transfer to the broad area. It was limited to the only injection site. We tested two types of AAVs including a CAG or CaMKII promoter. Different light responsivities elicited by ChR2 transferred cortex were recorded depending on the promoter the AAV included. These results indicated that it is important to choose appropriate promoter to transfer a gene into the cortex.
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