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The effects of the amino acid profile in tear fluid on ocular surface homeostasis and disease condition

Research Project

Project/Area Number 23659818
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

SOTOZONO Chie  京都府立医科大学, 医学(系)研究科(研究院), 講師 (30216585)

Co-Investigator(Kenkyū-buntansha) OKUMURA Naoki  同志社大学, 生命医科学部, 助教 (10581499)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアミノ酸 / 角膜 / 上皮細胞 / タイトジャンクション / 涙液 / ヒト角膜上皮細胞 / アルギニン / グルタミン酸 / グルタミン / アラニン / アスパラギン酸
Research Abstract

We examined the change of cell growth, wound healing, and barrier function of cultivated human corneal epithelial cells using specific culture medium including or deleting 1-23 amino acids. The proliferation of cultivated human corneal epithelial cells was inhibited by deleting Arg, Glu, Asn or Ala from the culture medium containing 23 amino acids (FULL medium). Arg deficit from culture medium also inhibited wound healing at the vitro wound-healing model. Transepithelial electrical resistance (TER) was high in FULL medium, and low in ZERO medium, indicating amino acids increase cell-cell adhesion. In an Arg deficit culture medium, TER dramatically decreased. In the Glu deficit culture medium and the Asn deficit culture medium, it also decreased.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2013 2012 2011

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (6 results)

  • [Journal Article] Visual Improvement after Cultivated Oral Mucosal Epithelial Transplantatio2013

    • Author(s)
      Sotozono C, Inatomi T, Nakamura T, Koizumi N, Yokoi N, Ueta M, Matsuyama K, Miyakoda K, Kaneda H, Fukushima M, Kinoshita S
    • Journal Title

      Ophthalmol, Peer-Reviewed papers

      Volume: 120(1) Issue: 1 Pages: 193-200

    • DOI

      10.1016/j.ophtha.2012.07.053

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of TGF-β signaling enables human corneal endothelial cell expansion in vitro for use in regenerative medicine.2013

    • Author(s)
      Okumura N, Kay EP, Nakahara M, Hamuro J, Kinoshita S, Koizumi N
    • Journal Title

      PLoS ONE

      Volume: 8(2) Issue: 2 Pages: e58000-e58000

    • DOI

      10.1371/journal.pone.0058000

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The ROCK Inhibitor Eye Drop Accelerates Corneal Endothelium Wound Healing.2013

    • Author(s)
      Okumura N, Koizumi N, Kay EP, Ueno M, Sakamoto Y, Nakamura S, Hamuro J, Kinoshita S
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 54(4) Issue: 4 Pages: 2493-2502

    • DOI

      10.1167/iovs.12-11320

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of prostaglandin E receptor subtype EP4 in conjunctival epithelium of patients with ocular surface disorders: case-control study2012

    • Author(s)
      Ueta M, Sotozono C, Yamada K, Yokoi N, Inatomi T, Kinoshita S
    • Journal Title

      BMJ Open, Peer-Reviewed papers

      Volume: 2(5) Issue: 5 Pages: e001330-e001330

    • DOI

      10.1136/bmjopen-2012-001330

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prostaglandin E2 Suppresses Poly I: C-Stimulated Cytokine Production Via EP2 and EP3 in Immortalized Human Corneal Epithelial Cells2012

    • Author(s)
      Ueta M, Matsuoka T, Sotozono C, Kinoshita S
    • Journal Title

      Cornea. Peer-Reviewed papers

      Volume: 31(11) Issue: 11 Pages: 1294-1298

    • DOI

      10.1097/ico.0b013e318242fd7c

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epistatic interaction between Toll-like receptor 3 (TLR3) and prostaglandin E receptor 3 (PTGER3) genes2012

    • Author(s)
      Ueta M, Tamiya G, Tokunaga K, Sotozono C, Ueki M, Sawai H, Inatomi T, Matsuoka T, Akira S, Narumiya S, Tashiro K, Kinoshita S
    • Journal Title

      J Allergy Clin Immunol Peer-Reviewed papers

      Volume: 129(5) Issue: 5 Pages: 1413-1416

    • DOI

      10.1016/j.jaci.2012.01.069

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of monocyte chemoattractant protein 1 expression by prostaglandin e2 in human ocular surface epithelium2012

    • Author(s)
      Ueta M, Sotozono C, Yokoi N, Kinoshita S
    • Journal Title

      ArchOphthalmol,Peer-Reviewed papers

      Volume: 130(2) Issue: 2 Pages: 249-251

    • DOI

      10.1001/archopthalmol.2011.1472

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The relation between visual performance and clinical ocular manifestations in stevens-johnson syndrome.Am J Ophthalmol2012

    • Author(s)
      Kaido M, Yamada M, Sotozono C,Kinoshita S, 他
    • Journal Title

      Peer-Reviewed papers

      Volume: 154(3) Issue: 3 Pages: 499-511

    • DOI

      10.1016/j.ajo.2012.03.044

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of new therapeutic modalities for corneal endothelial disease focused on the proliferation of corneal endothelial cells using animal models2012

    • Author(s)
      Koizumi N, Okumura N, Kinoshita S
    • Journal Title

      Exp Eye Res

      Volume: 95(1) Issue: 1 Pages: 60-67

    • DOI

      10.1016/j.exer.2011.10.014

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A selective inhibitor of the Rho kinase pathway, Y -27632, and its influence on wound healing in the corneal stroma2012

    • Author(s)
      Yamamoto M, Quantock AJ, Young RD, Okumura N, Ueno M, Sakamoto Y,Kinoshita S, Koizumi N
    • Journal Title

      Mol Vis

      Volume: 18 Pages: 1727-1739

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A ROCK inhibitor converts corneal endothelial cells into a phenotype capable of regenerating in vivo endothelial tissue.2012

    • Author(s)
      Okumura N, Koizumi N, Ueno M, et al
    • Journal Title

      Am J Pathol

      Volume: 181(7) Issue: 1 Pages: 268-277

    • DOI

      10.1016/j.ajpath.2012.03.033

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A selective inhibitor of the Rho kinase pathway, Y-27632, and its influence on wound healing in the corneal stroma.2012

    • Author(s)
      Yamamoto M, Quantock AJ, Young RD, Okumura N, Ueno M, Sakamoto Y,Kinoshita S,Koizumi N
    • Journal Title

      Mol Vis

      Volume: 18 Pages: 1727-1739

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amino Acid profiles in human tear fluids analyzed by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry2011

    • Author(s)
      Nakatsukasa M, Sotozono C, Shimbo K, Ono N, Miyano H, Okano A, Hamuro J, Kinoshita S
    • Journal Title

      Am J Ophthalmol

      Volume: 151(5) Issue: 5 Pages: 799-808

    • DOI

      10.1016/j.ajo.2010.11.003

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Etiologic Features of Stevens-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) with Ocular Involvement2012

    • Author(s)
      Sotozono C, Ueta M, Kinoshita S, Kitami A, Iijima M, Aihara M, Ikezawa Z, Kano Y, Shiohara T, Shirakata Y, Sakabayashi S, Matsubara Y, Hashimoto K
    • Organizer
      Annual Meeting of the American Academy of Ophthalmology
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2012-11-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Etiologic Features of Stevens-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) with Ocular Involvement.2012

    • Author(s)
      Sotozono C, Ueta M, Kinoshita S, Kitami A, Iijima M, Aihara M, Ikezawa Z, Kano Y, Shiohara T, Shirakata Y, Sakabayashi S, Matsubara Y, Hashimoto K
    • Organizer
      Annual Meeting of the American Academy of Ophthalmology
    • Place of Presentation
      Chicago, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Stevens-Johnson 症候群の病態と治療2011

    • Author(s)
      外園千恵
    • Organizer
      第117回京都眼科学会
    • Place of Presentation
      京都
    • Year and Date
      2011-07-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Stevens-Johnson 症候群、中毒性表皮壊死症(TEN)型薬疹と眼障害2011

    • Author(s)
      外園千恵
    • Organizer
      第115回日本眼科学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Stevens-Johnson症候群の病態と治療2011

    • Author(s)
      外園千恵
    • Organizer
      第117回京都眼科学会
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] Stevens-Johnson 症候群、中毒性表皮壊死症(TEN)型薬疹と眼障害2011

    • Author(s)
      外園千恵
    • Organizer
      第115回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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