Controlled release of granulocyte colony-stimulating factor (G-CSF) enhances osteoconductive and biodegradable properties of beta-tricalcium phosphate (beta-TCP) in a rat calvarial defect model

• Project/Area Number: 23659826
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Hokkaido University
• Principal Investigator: OYAMA Akihiko 北海道大学, 大学病院, 講師 (70374486)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Yuhei 北海道大学, 大学院医学研究科, 教授 (70271674) ; FURUKAWA Hiroshi 北海道大学, 大学院医学研究科, 講師 (00399924) ; FUNAYAMA Emi 北海道大学, 大学院医学研究科, 助教 (10533630) ; HAYASHI Toshikhiko 北海道大学, 大学院歯学研究科, 准教授 (00432146) ; SAITO Akira 北海道大学, 大学院医学研究科, 客員研究員 (70507574)
• Co-Investigator(Renkei-kenkyūsha): TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 唇顎口蓋裂 / 再生医療 / G-CSF / 徐放化 / β-TCP / 骨欠損 / ゼラチンハイドロゲル / 骨再生 / 骨再生医療 / バイオセラミックス

Research Abstract

A total of 27 calvarial defects were randomly divided into nine treatment groups. One set of defects was filled with a beta-TCP disc containing normal saline alone (group-A, control) or 1 (group-B), 5 (group-C), or 20 ug of G-CSF saline solution (group-D). Another set of defects was filled with a beta-TCP disc with an overlaid gelatin hydrogel sheet incorporating normal saline alone (group-E), or 1 (group-F), 5 (group-G), or 20 ug of G-CSF solution (group-H). The last set of defects was left untreated (group-I). Histologic and histomorphometric analyses were performed at eight weeks postoperatively.
In groups F and G, values of newly formed bone were significantly higher compared to group A. The percentage of remaining beta-TCP in group G was significantly lower compared to group A.

Research Products

• [Journal Article] Controlled Release of Granulocyte Colony-Stimulating Factor Enhances Osteoconductive and Biodegradable Properties of Beta-Tricalcium Phosphate in a Rat Calvarial Defect Model (2014)
  • Author(s): Minagawa.T, Tabata.Y, Oyama.A, Furukawa.H, Yamao.T, Yamamoto.Y
  • Journal Title: Int J Biomat Volume: Volume 2014 Pages: 11-11
  • Peer Reviewed
• [Journal Article] Controlled release of granulocyte colony-stimulating factor (G-CSF) enhances osteoconductive and biodegradable properties of beta-tricalcium phosphate in a rat calvarial defect model (2014)
  • Author(s): Tomohiro Minagawa, Yasuhiko Tabata, Akihiko Oyama, Hiroshi Furukawa, Takeshi Yamao, and Yuhei Yamamoto
  • Journal Title: International Journal of Biomaterials Volume: 2014 Pages: 1-11
  • Peer Reviewed
• [Presentation] Controlled release of G-CSF enhances osteoconductive and biodegradable properties of beta-TCP in a rat calvarial defect model (2013)
  • Author(s): 皆川知広,田畑泰彦,小山明彦,古川洋志,山尾健,山本有平
  • Organizer: 第22回日本形成外科基礎学術集会
  • Place of Presentation: 朱鷺メッセ(新潟市)
• [Presentation] 徐放化G-CSFはbeta-tricalcium phosphate (β-TCP)の骨伝導能および生体吸収性を増幅する (2013)
  • Author(s): 皆川知広，田畑泰彦，小山明彦，古川洋志，山尾健，山本有平
  • Organizer: 第22回日本形成外科学会基礎学術集会
  • Place of Presentation: 新潟コンベンションセンター（新潟県）
• [Presentation] 唇顎口蓋裂治療における再生医学～機能性scaffold を応用した顎裂再建 (2011)
  • Author(s): 皆川知広,田畑泰彦,小山明彦,古川洋志,山尾健,山本有平
  • Organizer: 23回北大形成外科アカデミー
  • Place of Presentation: 京王プラザホテル(札幌市)
  • Year and Date: 2011-12-10