Preparation of chondrocyte aggregates with gelatin microspheres to promote cartilage regeneration.
| Project/Area Number |
23659831
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
KAWAI Katsuya 京都大学, 医学(系)研究科(研究院), 准教授 (90273458)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 再生医学 / 国際情報交流 |
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| Research Abstract |
This study developed a novel 3D culture procedure; chondrocyte aggregates with gelatin microspheres to promote cartilage regeneration.The viability of chondrocytes and the sulfated glycosaminoglycan production of chondrocytes in an aggregate with microspheres were significantly higher compared with that of aggregate without microspheres. This result indicated that chondrocyte aggregates with microspheres enhanced chondrogenic differentiation in vitro. Moreover, in vivo implantation study, the area of regenerated cartilaginous tissue in the group of chondrocyte aggregates with microspheres was larger than that of chondrocyte aggregates without microspheres. In conclusion, this novel procedure may allow for the development of cartilage regeneration.
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Report
(4 results)
Research Products
(2 results)
