Making stem cells from fibroblasts by sphere formation

• Project/Area Number: 23659836
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: KISHI Kazuo 慶應義塾大学, 医学部, 教授 (40224919)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 組織培養 / 移植学 / 再生医療 / 毛包 / 幹細胞 / 培養 / 凝集

Research Abstract

By culturing adult murine fibroblasts on non-adhesive condition, it became possible to regenerate hair follicles. The present study tried to show how to make stem cells from fibroblasts by culturing non-adhesive culturing dish and make spheroids.

Research Products

• [Journal Article] Fetal skin possesses the ability toregenerate completely: complete regeneration of skin. (2012)
  • Author(s): Kishi K, Okabe K, Shimizu R, Kubota Y
  • Journal Title: Keio J Med Volume: 61(4) Pages: 101-8
  • Peer Reviewed
• [Journal Article] Skin graft (2012)
  • Author(s): Shimizu R, Kishi K.
  • Journal Title: Plast Surg Int. Volume: 2012 Pages: 563493-563493
  • DOI: 10.1155/2012/563493
  • Peer Reviewed
• [Journal Article] Fetal skin possess an ability to regenerate completely. (2012)
  • Author(s): Kazuo Kishi
  • Journal Title: Keio Journal of Medicine Volume: 61 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Sphere formation restores and confers hair-inducing capacity in cultured mesenchymal cells (2011)
  • Author(s): Shimizu R et al.(Kishi K ; 6人中6番)
  • Journal Title: Exp Dermatol Volume: 20 Issue: 8 Pages: 679-81
  • DOI: 10.1111/j.1600-0625.2011.01281.x
  • Peer Reviewed
• [Presentation] 線維芽細胞凝集塊形成による非対称性脱分化
  • Author(s): 清水瑠加、貴志和生ら
  • Organizer: 第20回日本形成外科学会基礎学術集会
  • Place of Presentation: ハイアットリージェンシー（東京都）