Research on cell rejuvenation by pluripotent cells

• Project/Area Number: 23659840
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: OKOCHI Hitoshi 独立行政法人国立国際医療研究センター, 細胞組織再生医学研究部, 部長 (30185235)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 再生医学 / 多能性幹細胞 / 幹細胞

Research Abstract

As cultured dermal papilla cells (DPC) lose hair-inducing ability, we co-cultured mouse DPC with mouse ES cells or iPS cells. We could not keep hair-inducing ability by these methods. Then we fused DPC with mouse ES cells. While fused cells behaved ES-like cells in ES media, they maintained some DPC specific gene expression in DPC culture media.

Research Products

• [Journal Article] Transcription factors interfering with dedifferentiation induce cell type-specific transcriptional profiles. (2013)
  • Author(s): Hikichi T, Matoba R, Ikeda T, Watanabe A, Yamamoto T, Yoshitake S, Tamura-Nakano M, Kimura T, Kamon M, Shimura M, Kawakami K, Okuda A, Okochi H, Inoue T, Suzuki A, Masui S
  • Journal Title: Proc Natl Acad Sci USA Volume: 110(16) Pages: 6412-7
• [Journal Article] Transcription factors interfering with dedifferentiation induce cell type-specific transcriptional profiles. (2013)
  • Author(s): Hikichi T, Matoba R, Ikeda T, Watanabe A, Yamamoto T, Yoshitake S, Tamura-Nakano M, Kimura T, Kamon M, Shimura M, Kawakami K, Okuda A, Okochi H, Inoue T, Suzuki A, Masui S.
  • Journal Title: Proceeding of the National Academy of Sciences of USA Volume: 110 Issue: 16 Pages: 6412-6417
  • DOI: 10.1073/pnas.1220200110
  • NAID: 120005244176
  • Peer Reviewed
• [Journal Article] Intracellular reactivation of transcription factors fused with protein transduction domain. (2011)
  • Author(s): Konno M, Masui S, Hamazaki TS, Okochi H
  • Journal Title: J Biotechnol Volume: 154 Pages: 298-303
• [Journal Article] Intracellular reactivation of transcription factors fused with protein transduction domain (2011)
  • Author(s): Konno M, Masui S, Hamazaki TS, Okochi H
  • Journal Title: J Biotechnol Volume: 154 Pages: 298-303
  • Peer Reviewed
• [Presentation] 真皮の細胞を用いた再生医学 (2011)
  • Author(s): 大河内仁志
  • Organizer: 第20回日本形成外科学会基礎学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-06
• [Presentation] 真皮の細胞を用いた再生医学 (2011)
  • Author(s): 大河内仁志
  • Organizer: 第２０回日本形成外科学会基礎学術集会(招待講演)
  • Place of Presentation: 東京
• [Book] 組織幹細胞 「再生医療叢書1 幹細胞」 山中伸弥、中内啓光編 (2012)
  • Author(s): 大河内仁志
  • Publisher: 朝倉書店
• [Book] Adult Stem Cell: Sources and Characterization Tissue Engineering- From lab to Clinic. (edited by Norbert Pallua and Christopf V. Suschek) (2011)
  • Author(s): Hitoshi Okochi
  • Publisher: Springer
• [Book] Tissue Engineering- From lab to Clinic (2011)
  • Author(s): Hitoshi Okochi (edited by Norbert Pallua and Christopf V. Suschek)
  • Total Pages: 634
  • Publisher: Springer