Involvement of claudin-network in the development of ARDS caused by influenza virus infection

• Project/Area Number: 23659841
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Tohoku University
• Principal Investigator: KAWAKAMI Kazuyoshi 東北大学, 大学院・医学系研究科, 教授 (10253973)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazuo 千葉大学, 大学院・医学研究院, 特任教授 (20192130)
• Co-Investigator(Renkei-kenkyūsha): AOYAGI Tetsuji 東北大学, 大学院・医学系研究科, 助教 (50581609)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ARDS / インフルエンザ / タイトジャンクション / クローディン / 気管支上皮細胞 / 血管内皮細胞 / 炎症細胞

Research Abstract

We addressed the possible involvement of claudins in the development of acute lung injury. In a real-time PCR analysis, expression of cluadin 2 and 4 was increased in the injured lungs, whereas that of claudin 5, 6, 9 and 18-1 was reduced. In an immunohistochemical analysis, expression of claudin 2 and 4 was detected in the bronchoepithelial cells of injured lungs. Lung edema caused by LPS administration tended to be worsened in claudin4 gene-disrupted mice. These results suggest that claudins may be involved in thedevelopment of ARDS.

Research Products

• [Journal Article] Involvement of High Mobility Group Box 1 and the therapeutic effect of recombinant thrombomodulin in a mouse model of severe acute respiratory distress syndrome (2013)
  • Author(s): Kudo D, Toyama M, Aoyagi T, Akahori Y, Yamamoto H, Ishii K, Kanno E, Maruyam R, Kaku M, Kushimoto S, Kawakami K
  • Journal Title: Clin. Exp. Immunol Volume: (In press) Issue: 2 Pages: 276-87
  • DOI: 10.1111/cei.12106
  • Peer Reviewed
• [Journal Article] Low-dose Interferon-α Treatment Improves Survival and Inflammatory Responses in a Mouse Model of Fulminant Acute Respiratory Distress Syndrome. Inflammation (2013)
  • Author(s): Kudo D, Uno K, et al
  • Journal Title: Inflammation Volume: 14 Issue: 4 Pages: 1-8
  • DOI: 10.1007/s10753-013-9607-1
  • Peer Reviewed
• [Journal Article] Contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection. Microbiol (2012)
  • Author(s): Sugamata R, Dobashi H, Nagao T, Yamamoto K, Nakajima N, Sato Y, Aratani Y, Oshima M, Sata T, Kobayashi K, Kawachi S, Nakayama T, Suzuki K
  • Journal Title: Immunol Volume: 56 Pages: 171-182
  • Peer Reviewed
• [Journal Article] Contribution of neutrophil-derived myeloperoxidase in the early phase of fulminant acute respiratory distress syndrome induced by influenza virus infection (2012)
  • Author(s): Sugamata R et al.
  • Journal Title: Microbiology and Immunology Volume: 56 Issue: 3 Pages: 171-182
  • DOI: 10.1111/j.1348-0421.2011.00424.x
  • Peer Reviewed
• [Journal Article] 病態生理,第2章病理・病態生理「新しい診断と治療のABC肺炎改訂第2版」 (2011)
  • Author(s): 工藤大介,久志本成樹,川上和義
  • Journal Title: 最新医学別冊,最新医学社 Pages: 39-45
• [Presentation] マウス劇症型ARDSモデルに対するrTMの治療効果とその作用機序 (2013)
  • Author(s): 工藤大介,外山真彦,石井恵子,久志本成樹,川上和義
  • Organizer: 第35回日本血栓止血学会学術集会
  • Place of Presentation: 山形
• [Presentation] Contributions of High Mobility Group Box 1 in experimental fulminant acute respiratory distress Syndrome (2012)
  • Author(s): Kudo D, Ishii K, Kushimoto S, Kawakami K
  • Organizer: 7th Congress of the International Federation of Shock Societies and 35th Annual Conference on Shock
  • Place of Presentation: Miami Beach, FL, USA
• [Presentation] Contributions of High Mobility Group Box 1 in experimental fluminant acute respiratory distress syndrome (2012)
  • Author(s): Daisuke Kudo他
  • Organizer: 7th Congress of the international Federation of Shock Societies and 35th Annual Conference on Shock
  • Place of Presentation: Miami Beach, FL, USA
• [Presentation] Anti-inflammatory and therapeutic effects of interferon-α in a mouse model of acute respiratory distress syndrome (2011)
  • Author(s): Kudo D, Uno K, Ishii K, Kushimoto S, Kawakami K
  • Organizer: 第40回日本免疫学会学 術集会
  • Place of Presentation: 千葉
• [Presentation] Anti-inflammatory and therapeutic effects of interferon-α in a mouse model of acute respiratory distress syndrome (2011)
  • Author(s): Kudo D et al.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Remarks] 東北大学大学院医学系研究科感染分子病態解析学分野
  • URL: http://www.infect-immun.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://www.infect-immun.med.tohoku.ac.jp/