The involvement and their molecular mechanism of microRNAs in the invasion of oral cancer

• Project/Area Number: 23659858
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: The University of Tokushima (2012); Hiroshima University (2011)
• Principal Investigator: KUDO Yasusei 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (50314753)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 口腔病理学 / microRNA / 口腔癌 / 浸潤 / 上皮間葉移行（EMT） / EMT

Research Abstract

We identified miR-203 as an invasion related microRNA (miRNA) by comparing miRNA expression profile between oral cancer cell line and its highly invasive clone. Moreover, we identified NUAK and SNAI2 as a target mRNA of miR-203.

Research Products

• [Journal Article] Matrix metalloproteinase-13 directly and indirectly promotes tumor angiogenesis (2012)
  • Author(s): Kudo, Y.*, Iizuka, S., Yoshida, M., Tsunematsu, T., Subarnbhesaj, A., Siriwardena, S.B.S.M., Tahara, H., Ogawa, I., Takata, T
  • Journal Title: J Biol Chem Volume: 287 Pages: 38716-38728
  • Peer Reviewed
• [Journal Article] Periostin directly and indirectly promotes tumor lymphangiogenesis of head and neck cancer (2012)
  • Author(s): Kudo Y, Iizuka S, Yoshida M, Nguyen PT, Siriwardena SB, Tsunematsu T, Ohbayashi M, Ando T, Hatakeyama D, Shibata T, Koizumi K, Maeda M, Ishimaru N, Ogawa I, Takata T
  • Journal Title: PLoS One Volume: 7(8) Issue: 8 Pages: e44488-e44488
  • DOI: 10.1371/journal.pone.0044488
  • NAID: 120006714446
  • Peer Reviewed
• [Journal Article] The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma (2012)
  • Author(s): Desrochers, T.M., Shamis, Y., Alt-Holland, A., Kudo, Y., Takata, T., Wang, G., Jackson-Grusby, L., Garlick, J.A
  • Journal Title: Epigenetics Volume: 7 Pages: 34-46
• [Journal Article] Matrix metalloproteinase-13 directly and indirectly promotes tumor angiogenesis. (2012)
  • Author(s): Kudo, Y., et al.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 38716-38728
  • Peer Reviewed
• [Journal Article] Periostin promotes tumor lymphangiogenesis in a direct and an indirect manner. (2012)
  • Author(s): Kudo, Y., , et al.
  • Journal Title: PLoS ONE Volume: 7
  • Peer Reviewed
• [Journal Article] The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma. (2012)
  • Author(s): Desrochers, T.M., , et al.
  • Journal Title: Epigenetics Volume: 7 Pages: 34-46
  • Peer Reviewed
• [Journal Article] MMP10/stromelysin-2 promotes invasion of head and neck cancer (2011)
  • Author(s): Deraz EM, Kudo Y, Yoshida M, Tani H, Tsunematsu T, et al
  • Journal Title: PLos ONE Volume: (電子媒体) Issue: 10 Pages: e25438-e25438
  • DOI: 10.1371/journal.pone.0025438
  • Peer Reviewed
• [Journal Article] miR-22 represses cancer progression by inducing celular senescence (2011)
  • Author(s): Xu, D., Takeshita, F., Hino, Y., Fukunaga, S., Kudo, Y., Tamaki, A., Takata, T., Shimamoto, A., Ochiya, T., Tahara, H
  • Journal Title: J Cell Biol Volume: 193(2) Pages: 409-424
  • Peer Reviewed
• [Journal Article] MMP-10/stromelysin-2 promotes invasion of head and neck cancer. (2011)
  • Author(s): Deraz EM, Kudo Y, Yoshida M, Obayashi M, Tsunematsu T, Tani H, Siriwardena SB, Keikhaee MR, Qi G, Iizuka S, Ogawa I, Campisi G, Lo Muzio L, Abiko Y, Kikuchi A, Takata T.
  • Journal Title: PLoS ONE Volume: 6
  • NAID: 120005300499
  • Peer Reviewed
• [Journal Article] miR-22 represses cancer progression by inducing cellular senescence. (2011)
  • Author(s): Xu D, Takeshita F, Hino Y, Fukunaga S, Kudo Y, Tamaki A, Matsunaga J, Takahashi RU, Takata T, Shimamoto A, Ochiya T, Tahara H.
  • Journal Title: J Cell Biol. Volume: 193 Pages: 409-424
  • Peer Reviewed
• [Presentation] FGFR-1 inhibitor PD173074 induces mesenchymal-epithelial tramsition through suppression of AP-1 in Head and Neck Squamous Cell Carcinoma (2012)
  • Author(s): Nguyen PT, Inubushi T, Tsunematsu T, Kudo Y, Kamata N, Ogawa I, Takata T
  • Organizer: 第23回日本臨床口腔病理学会・学術大会
  • Place of Presentation: 東京都(東京医科歯科大学)
  • Year and Date: 2012-08-30
• [Presentation] 口腔癌における浸潤抑制因子としてのmiR-203 の同定 (2012)
  • Author(s): 大林真理子,吉田真希,工藤 保誠, 高田 隆
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市(ロイトン札幌)
• [Presentation] 口腔癌における浸潤抑制因子としてのmiR-203の同定 (2012)
  • Author(s): 大林真理子，他
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌ロイトンホテル（北海道）
• [Presentation] FGFR-1 inhibitor PD173074 induces mesenchymal-epithelial tramsition through suppression of AP-1 in Head and Neck Squamous Cell Carcinoma. (2012)
  • Author(s): Nguyen PT, , et al.
  • Organizer: 第23回日本臨床口腔病理学会・学術大会
  • Place of Presentation: 東京医科歯科大学（東京都）
• [Presentation] Identification of microRNA-203 as an inhibitor of invasion in oral cancer (2011)
  • Author(s): Obayashi M, Kudo Y, Yoshida M, Hino Y, Fukunaga S, Tahara H, Takata T
  • Organizer: 59thJADR Annual Meeting
  • Place of Presentation: 広島市(広島国際会議場)
  • Year and Date: 2011-08-09
• [Presentation] 口腔癌における浸潤抑制因子としてのmiR-203 の同定 (2011)
  • Author(s): 大林真理子,工藤保誠,吉田真希,日野由美子,福永早央里,田原栄俊,高田 隆
  • Organizer: 第3回 RNAi 研究会
  • Place of Presentation: 広島市(広島プリンスホテル)
• [Presentation] 口腔癌における浸潤抑制因子としてのmiR-203 の同定 (2011)
  • Author(s): 大林真理子,工藤保誠,吉田真希,日野由美子,福永早央里,田原栄俊,高田 隆
  • Organizer: 第30回分子病理学研究会
  • Place of Presentation: 倉敷市(下電ホテル)