| Project/Area Number |
23659859
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Hidetaka 九州大学, 大学院歯学研究科, 教授 (80136499)
NAGATA Kengo 九州大学, 大学院歯学研究科, 助教 (90189134)
WADA Hiroko 九州大学, 大学院歯学研究科, 助教 (70380706)
KOBAYASHI Ieyoshi 九州大学, 大学院歯学研究科, 准教授 (40243951)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 歯の再生 / 組織再構築 / 歯胚形成 / 歯原性幹細胞 |
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| Research Abstract |
The recombination of cells obtained from tooth germs successfully develop into teeth in previous studies. However, it is difficult to use human tooth germ as a cellular seed because of ethical issues. We attempted establishment of odontogenic stem cells by regulation of gene "X" expression. We herein transfected Thymosin beta 4 (Tb4) expression vector, as a gene "X" expression, into non-odontogenic HaCaT cells in this study because Tb4 has been reported to be closely related to the initiation and development of the tooth germ. Tb4-transfected cells formed nodules with calcium phosphate in calcification-inducing medium. Selected clones with larger amounts of calcium deposits expressed the upregulation of odontogenesis-related genes. These proteins were immunohistochemically observed in in vitro and vivo samples. This study demonstrated the possibility of induction of dental epithelial cell differentiation marker gene expression in non-odontogenic HaCaT cells by Tb4.
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