Epigenetic changes in cell fate decision during mesenchymal cell differentiation.

• Project/Area Number: 23659862
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: NIFUJI Akira 鶴見大学, 歯学部, 教授 (00240747)
• Co-Investigator(Kenkyū-buntansha): NAKASHIMA Kazuhisa 鶴見大学, 歯学部, 非常勤講師 (90252692) ; ARAKI Ryoko 放射線医学総合研究所, 重粒子センター, 研究員 (40392211)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞分化 / 間葉系細胞 / メチル化 / エピジェネティクス / エピジェネティック解析 / 転写因子

Research Abstract

We examined epigenetic changes during cell fate change of mesenchyal cells, using 10T1/2fibroblastic cells. Aza-cytidine treatment induced myogenic differentiation whereas HDAC-inhibitor TSA blocked it. TSA also blocked ER-myoD induced myogenesis. Bisulfate sequence and targeted amplicon using sequence next generation reveled that myoD induced myogenin expression does not accompanied with change of CpG methylation irrespective of presence of TSA.

Research Products

• [Journal Article] expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development. (2013)
  • Author(s): Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, Nifuji A. Predominant
  • Journal Title: Gene Expr Patterns. Volume: 13 Pages: 84-90
  • Peer Reviewed
• [Journal Article] Negligible immunogenicity of terminallydifferentiated cells derived from iPSCs orESCs. (2013)
  • Author(s): Araki R, Uda M, Hoki Y, Sunayama M, Nakamura M, Ando S, Sugiura M, Ideno H, Shimada A, Nifuji A, Abe M.
  • Journal Title: Nature 494 Pages: 100-104
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of skeletal tissue formation. (2013)
  • Author(s): Nifuji A
  • Journal Title: J Physical Fitness and Sports Medicine. Volume: 2 (1) Pages: 1-8
  • NAID: 10031159011
  • URL: https://www.jstage.jst.go.jp/article/jpfsm/2/1/2_1/_article
  • Peer Reviewed
• [Journal Article] Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells (2013)
  • Author(s): Ryoko Araki
  • Journal Title: Nature Volume: 494 Issue: 7435 Pages: 100-104
  • DOI: 10.1038/nature11807
  • Peer Reviewed
• [Journal Article] Predominant expression of H3K9 methyltransferases in prehypertrophic and hypertrophic chondrocytes during mouse growth plate cartilage development (2013)
  • Author(s): Ideno H, Shimada A, Imaizumi K, Kimura H, Abe M, Nakashima K, and Nifuji A
  • Journal Title: Gene Expr Pat Volume: 13 Issue: 3-4 Pages: 84-90
  • DOI: 10.1016/j.gep.2013.01.002
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of skeletal tissue formation (2013)
  • Author(s): Nifuji A
  • Journal Title: J Phys Fitness Sports Med, Volume: 2 Pages: 1-8
  • NAID: 10031159011
  • Peer Reviewed
• [Journal Article] Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue. (2012)
  • Author(s): Shimada A, Komatsu K, Nakashima K, Poschl E, Nifuji A.
  • Journal Title: Histochem Cell Volume: Biol.137 Pages: 841-847
  • Peer Reviewed
• [Journal Article] Comprehensive gene expression analyses in pluripotent stem cells of a planarian, Dugesia japonica (2012)
  • Author(s): Shibata, N., Hayashi, T., Fukumura, R., Fujii, J., Kudome-Takamatsu, T., Nishimura, O., Sano, S., Son, F., Suzuki, N., Araki, R., Abe, M., Agata, K.
  • Journal Title: International Journal of Developmental Biology Volume: 56 Issue: 1-2-3 Pages: 93-102
  • DOI: 10.1387/ijdb.113434ns
  • Peer Reviewed
• [Journal Article] A Simultaneous Space-sampling Method for DNA Fraction Collection Using a Comb Structure in Microfluidic Devices (2011)
  • Author(s): Zheyu Li
  • Journal Title: Electrophoresis Volume: 32 Issue: 23 Pages: 3392-3398
  • DOI: 10.1002/elps.201100362
  • Peer Reviewed
• [Journal Article] Crucial role of c-Myc in the generation of induced pluripotent stem cells. (2011)
  • Author(s): Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M
  • Journal Title: Stem Cells. Volume: 29(9) Issue: 9 Pages: 1362-70
  • DOI: 10.1002/stem.685
  • Peer Reviewed
• [Journal Article] ERK signaling controls blastema cell differentiation during planarian regeneration. (2011)
  • Author(s): Tasaki Junichi
  • Journal Title: Development Volume: 138 Issue: 12 Pages: 2417-27
  • DOI: 10.1242/dev.060764
  • Peer Reviewed
• [Presentation] Limited and comparable immunogenicity of terminally differentiated cells derived from both iPSCs and ESCs (2012)
  • Author(s): Uda M, Araki R, Hoki Y, Sayama M, Nakamura M, Andou S, Sugiura M, IdenoH, Shimada A, Nifuji A, Abe M.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2012-12-13
• [Presentation] Expression of three histone 3 lysine 9 methyltransferases duringdevelopment of mouse molar (2012)
  • Author(s): Kamiunten T, Shimada A, Ideno H, Nakamura Y, Kimura H, Nakashima K, Nifuji A.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2012-12-13
• [Presentation] Soluble factors may mediate signals from tendons to bone (2012)
  • Author(s): Wada S, Shimada A, Ideno H, Nakamura Y, Nakashima K, Nifuji A.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2012-12-13
• [Presentation] Histone 3 lysine 9 methyltransferases G9a, GLP and SETDB1 are predominantly expressed in the prehypertrophc chondrocytes duringthe growth plate chondrocyte development (2012)
  • Author(s): Ideno H, Shimada A, Kamiunten T, Imaizumi K, Kimura H, Nakashima K, Nifuji A.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2012-12-11
• [Presentation] Region-specific CpG demethylation patterns of the myogenin promoter isrequired for MyoD mediated myogenic conversion from fibroblasts into myoblasts (2012)
  • Author(s): Ideno H, Nakashima K, Imaizumi K, Kasama Y, Araki R, Abe M, Nifuji A.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2012-12-11
• [Presentation] MyoD-induced myogenic conversion requires cell proliferation (2012)
  • Author(s): 出野 尚、中島 和久、荒木 良子、今泉 和彦、二藤 彰
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 長良川国際会議場 岐阜
  • Year and Date: 2012-09-14
• [Presentation] TSAによるmyogeninプロモーター脱メチル化 制御を介した筋芽細胞への分化転換の抑制 (2012)
  • Author(s): 出野 尚、中島和久、荒木良子、今泉 和 彦、安倍 真澄、二藤 彰
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2012-07-20
• [Presentation] アレンドロネートの細胞内取込みによる骨形成の促進 (2012)
  • Author(s): 小松浩一郎、島田 明美、出野 尚、柴田 達也、中島和久、二藤 彰.
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2012-07-19
• [Presentation] アレンドロネートの細胞内取込みによる骨形成の促進 (2012)
  • Author(s): 小松浩一郎
  • Organizer: 第３０回日本骨代謝学会
  • Place of Presentation: 東京 京王プラザホテル
• [Presentation] TSAによるmyogeninプロモーター脱メチル化制御を介した筋芽細胞への分化転換の抑制 (2012)
  • Author(s): 出野 尚
  • Organizer: 第３０回日本骨代謝学会
  • Place of Presentation: 東京 京王プラザホテル
• [Presentation] MyoD-induced myogenic conversion requires cell proliferation (2012)
  • Author(s): 出野 尚
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 岐阜県 長良川国際会議場
• [Presentation] Region-specific CpG demethylation patterns of the myogenin promoter is required for MyoD mediated myogenic conversion from fibroblasts into myoblasts (2012)
  • Author(s): Hisashi Ideno
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡県 福岡国際会議場
• [Presentation] Listone 3 lysine 9 methyltransferases G9a, GLP and Setdb1 are predominantly expressed in the prehypertrophc chondrocytes during the growth plate chondrocyte development distribution (2012)
  • Author(s): Hisashi Ideno
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡県 福岡国際会議場
• [Presentation] Soluble factors may mediate signals from tendons to bone (2012)
  • Author(s): Satoshi Wada
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡県 福岡国際会議場
• [Presentation] Expression of three histone 3 lysine 9 methyltransferases during development of mouse molar. (2012)
  • Author(s): Taichi Kamiunten
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡県 福岡国際会議場
• [Presentation] The myogenic conversion from fibroblasts into myogenic cells is blocked by Trichostatin A through suppression of myogenin expression (2011)
  • Author(s): Ideno H, Araki R,Imaizumi K, Abe M, Nifuji A.
  • Organizer: 第34回日本分子生物 学会年会
  • Place of Presentation: パシフィコ横浜 会議センター、神奈川
  • Year and Date: 2011-12-16
• [Presentation] Pre-incubation of whole teeth in collagen gel enrich progenitor cells of mouse periodontal ligament. 第 (2011)
  • Author(s): Shimada A, Shibata T, Komatsu K, IdenoH, Nifuji A.
  • Organizer: 34回日本分 子生物学会年会
  • Place of Presentation: パシフィコ横浜会議センター、神奈川
  • Year and Date: 2011-12-13
• [Presentation] DNAメチル化阻害剤とHDAC 阻害剤による筋細胞分化制御 (2011)
  • Author(s): 出野 尚、荒木 良子、今泉 和彦、安倍 真澄、二藤 彰
  • Organizer: 第66回体力医学会大会
  • Place of Presentation: 下関市海峡メッセ,山口
  • Year and Date: 2011-09-17
• [Presentation] 歯周組織形成過程におけるAnnexin a5の発現と機能 (2011)
  • Author(s): 島田 明美、小松浩一郎、中島 和久、網塚 憲生、Bent Brachvogel、二藤 彰
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場,大阪
  • Year and Date: 2011-07-28
• [Presentation] Nifuji A. Annexin a5 Expression Regulates Periodontal Tissue and Cementum Formation (2010)
  • Author(s): Shimada A, Komatsu K, Nakashima K, Amizuka N, Brachvogel B
  • Organizer: 2011 Annual Meeting of ASBMR
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2010-09-19
• [Presentation] New aspects of bone formation
  • Author(s): Akira Nifuji
  • Organizer: Seminar for the 65th anniversary of Dankook University
  • Place of Presentation: Dankook University, Korea
  • Invited
• [Presentation] The myogenic conversion from fibroblasts into myogenic cells is blocked by Trichostatin A through suppression of myogenin expression
  • Author(s): Hisashi Ideno、Ryoko Araki、Kazuhiko Imaizumi、Masumi Abe、Akira Nifuji
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県 パシフィコ横浜
• [Presentation] 出野 尚、荒木 良子、今泉 和彦、安倍 真澄、二藤 彰
  • Author(s): DNAメチル化阻害剤とHDAC阻害剤による筋細胞分化制御
  • Organizer: 第66回日本体力医学会
  • Place of Presentation: 山口県 下関市海峡メッセ