Epigenetic changes in cell fate decision during mesenchymal cell differentiation.
Project/Area Number |
23659862
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Morphological basic dentistry
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Research Institution | Tsurumi University |
Principal Investigator |
NIFUJI Akira 鶴見大学, 歯学部, 教授 (00240747)
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Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Kazuhisa 鶴見大学, 歯学部, 非常勤講師 (90252692)
ARAKI Ryoko 放射線医学総合研究所, 重粒子センター, 研究員 (40392211)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 細胞分化 / 間葉系細胞 / メチル化 / エピジェネティクス / エピジェネティック解析 / 転写因子 |
Research Abstract |
We examined epigenetic changes during cell fate change of mesenchyal cells, using 10T1/2fibroblastic cells. Aza-cytidine treatment induced myogenic differentiation whereas HDAC-inhibitor TSA blocked it. TSA also blocked ER-myoD induced myogenesis. Bisulfate sequence and targeted amplicon using sequence next generation reveled that myoD induced myogenin expression does not accompanied with change of CpG methylation irrespective of presence of TSA.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Negligible immunogenicity of terminallydifferentiated cells derived from iPSCs orESCs.2013
Author(s)
Araki R, Uda M, Hoki Y, Sunayama M, Nakamura M, Ando S, Sugiura M, Ideno H, Shimada A, Nifuji A, Abe M.
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Journal Title
Nature 494
Pages: 100-104
Related Report
Peer Reviewed
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[Journal Article] Comprehensive gene expression analyses in pluripotent stem cells of a planarian, Dugesia japonica2012
Author(s)
Shibata, N., Hayashi, T., Fukumura, R., Fujii, J., Kudome-Takamatsu, T., Nishimura, O., Sano, S., Son, F., Suzuki, N., Araki, R., Abe, M., Agata, K.
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Journal Title
International Journal of Developmental Biology
Volume: 56
Issue: 1-2-3
Pages: 93-102
DOI
Related Report
Peer Reviewed
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[Journal Article] Crucial role of c-Myc in the generation of induced pluripotent stem cells.2011
Author(s)
Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M
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Journal Title
Stem Cells.
Volume: 29(9)
Issue: 9
Pages: 1362-70
DOI
Related Report
Peer Reviewed
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[Presentation] Limited and comparable immunogenicity of terminally differentiated cells derived from both iPSCs and ESCs2012
Author(s)
Uda M, Araki R, Hoki Y, Sayama M, Nakamura M, Andou S, Sugiura M, IdenoH, Shimada A, Nifuji A, Abe M.
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場、福岡
Year and Date
2012-12-13
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