Radiosensitization of oral cancer cells by expressing abnormalHuntingtin

Research Project

Project/Area Number	23659876
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Pathobiological dentistry/Dental radiology
Research Institution	Tokyo Medical and Dental University
Principal Investigator	MIURA Masahiko 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (10272600)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	実験腫瘍学 / 放射線増感
Research Abstract	The purpose of this study was to explore the hypothesis that tumor cells should be radiosensitized by expressing the abnormal Hungtingtin protein because the repair of DNA double strand breaks (DSB) is reported to be inhibited in neurons expressing such a protein due to exhaustion of Ku70 essential for the DSB repair. We found that tumor cells forming nuclear inclusion bodies exhibit inhibition of DSB repair and radiosensitization. Efficient expression must be obtained as a radiosensitizing strategy and further study is required.