| Project/Area Number |
23659882
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kumamoto University |
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Principal Investigator |
WILSON MASAYO 熊本大学, 大学院生命科学研究部, 研究員 (90271113)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Norie 熊本大学, 大学院生命科学研究部, 准教授 (80253722)
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| Co-Investigator(Renkei-kenkyūsha) |
IRIE Atushi 熊本大学, 大学院生命科学研究部, 講師 (30250343)
TASHIRO Kosuke 九州大学, (連合)農学研究科(研究院), 准教授 (00192170)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | がん / 転移 / 転移性がん細胞マーカー / マイクロメタ / シグナルネットワーク / プロテオミクス / マイクロアレイ / 転移性癌細胞マーカー / 癌 / シグナル伝達 |
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| Research Abstract |
This study aimed to establish a detection system for micro-metastasis and metastatic stages using examination of biopsy. We created an in vivo animal model of heterogeneous human cancer consisting of non-metastatic (NM) and metastatic (M) tongue cancer cells and analyzed the pattern of metastasis and growth of both cancer cell lines. In addition, using integrated differential proteomics and transcriptomics, we identified significant activation of hypoxia-inducible-related signals in M cancer cells versus NM cancer cells. The analysis of the expression pattern of these molecules in an in vivo animal model suggested that we may be able to determine the metastatic stages of cancer by comprehensive examination ofchanges in the pattern of their expression (localization, expression volume).
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