Effects of soluble carbodiimide on the strengthening mechanism of human dentin by collagen cross-linking

• Project/Area Number: 23659888
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: HAYASHI Mikako 大阪大学, 歯学研究科(研究院), 教授 (40271027)
• Co-Investigator(Renkei-kenkyūsha): 斎藤 充 東京慈恵会医科大学, 医学部, 准教授 (50301528)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 保存修復学 / 象牙質 / 機械的強度 / 水溶性カルボジイミド / コラーゲン / 歯学 / 架橋 / 分子間架橋

Research Abstract

The strengthening effects of human dentin by soluble carbodiimide were investigated with an aim of preventing tooth fracture. We found that soluble carbodiimide was significantly effective to improve the mechanical strengths of dentin. The strengthening mechanism of dentin by heating was found to promote new collagen cross-linking with relation to water molecules.

Research Products

• [Journal Article] Detection of dentinal microcracks using infrared thermography (2013)
  • Author(s): Matsusita-Tokugawa M
  • Journal Title: Journal of Endodontics Volume: 39 Issue: 1 Pages: 88-91
  • DOI: 10.1016/j.joen.2012.06.033
  • Peer Reviewed
• [Journal Article] う蝕象牙質除去後の残存細菌にEr: YAG レーザーが与える影響 (2013)
  • Author(s): 高橋雄介
  • Journal Title: 日本歯科保存学雑誌 Volume: 56 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Effects of heating on the mechanical and chemical properties of human dentin (2012)
  • Author(s): Hayashi M, Furuya Y, Minoshima K, Saito M, Marumo K, NakashimaS, HongoC, KimJ, Ota T, Ebisu S
  • Journal Title: Dental Materials Volume: 28 Issue: 4 Pages: 385-391
  • DOI: 10.1016/j.dental.2011.11.015
  • Peer Reviewed
• [Journal Article] Clinical guidelines for treating caries in adults following a minimal intervation policy-Evidence and consensus based report (2012)
  • Author(s): Momoi Y, Hayashi M, 他
  • Journal Title: Journal of Dentistry Volume: 40 Pages: 95-105
  • Peer Reviewed
• [Journal Article] How regular visits and preventive programs affect onset of adult caries (2012)
  • Author(s): Ito A, Hayashi M, Hamasaki T, Ebisu S
  • Journal Title: Journal of Dental Research Volume: 91 Issue: 7_suppl Pages: S52-S58
  • DOI: 10.1177/0022034511435701
  • Peer Reviewed
• [Journal Article] Clinical guidelines for treating caries in adults following a minimal intervention policy-Evidence and consensus based report (2012)
  • Author(s): Hayashi, et.al.
  • Journal Title: Journal of Dentistry Volume: 40 Issue: 2 Pages: 95-105
  • DOI: 10.1016/j.jdent.2011.10.011
  • NAID: 120006986655
  • Peer Reviewed
• [Journal Article] Risk assessment of dental caries by using Classification and Regressi on Trees (2011)
  • Author(s): Hayashi, et.al
  • Journal Title: Journal of Dentistry Volume: 39 Issue: 6 Pages: 457-463
  • DOI: 10.1016/j.jdent.2011.04.002
  • Peer Reviewed
• [Journal Article] Ways of enhancing pulp preservation by stepwise excavation -A systematic review (2011)
  • Author(s): Hayashi M, Fujitani M, Yamaki C, Momoi Y
  • Journal Title: Journal of Dentistry Volume: 39 Issue: 2 Pages: 95-107
  • DOI: 10.1016/j.jdent.2010.10.012
  • Peer Reviewed
• [Journal Article] Relationship between laser fluorescence and bacterial invasion in arrested dentinal carious lesions (2011)
  • Author(s): Iwami Y, Yamamoto H, Hayashi M, Ebisu S
  • Journal Title: Lasers in Medicine and Science Volume: 26 Issue: 4 Pages: 439-444
  • DOI: 10.1007/s10103-010-0798-5
  • Peer Reviewed
• [Presentation] バイオメカニクスの視点でとらえる高齢者の歯根破折 (2012)
  • Author(s): 林美加子
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] 慢性う蝕の進行状態の評価におけるう蝕歯質の硬さ測定の有用性 (2012)
  • Author(s): 岩見行晃,山本洋子,林美加子
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] Effect of degraded dentin matrix proteins induced by matrix metalloproteinase molecules on pulp cells function. (2012)
  • Author(s): Takahashi Y, Okamoto M, Yoshioka S, Hayashi M
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] Development of rechargeable CPC-loaded hydrogel particles for long-term delivery of antimicrobials. (2012)
  • Author(s): Kitagawa H, Imazato S, Hayashi M
  • Organizer: The First Japan-Thailand-Korea Joint Symposium on Translational Research in Oral Sciences
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2012-11-16
• [Presentation] 口腔外傷治療最前線「歯根破折への保存的対応 vs 外科的対応」 (2012)
  • Author(s): 林美加子
  • Organizer: 日本歯科医学会総会分科会プログラム (日本外傷歯学会)
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-12
• [Presentation] 臨学一体の日本歯内療法学会がお知らせする最新の歯内療法「先進医療: X線CT画像診断に基づく手術用顕微鏡を用いた歯根端切除手術」 (2012)
  • Author(s): 林美加子
  • Organizer: 日本歯科医学会総会分科会プログラム (日本歯内療法学会)
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-11
• [Presentation] Dispersal of Porphyromonas gingivalis biofilm induced by environmental factors and proteases. (2012)
  • Author(s): Yamaguchi M, Noiri Y, Asahi Y, Maezono H, Yamamoto R, Kuremoto K, Hayashi M, Ebisu S
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, USA.
  • Year and Date: 2012-10-03
• [Presentation] 紫外線照射による象牙質強化効果メカニズムの化学的解 析 (2012)
  • Author(s): 古谷優,武田侑子,和田陽子,辻本恭久,林美加子,恵比須繁之
  • Organizer: 第136回日本歯科保存学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-29
• [Presentation] 光透過型ファイバーポスト修復の根部における接着状態の評価 (2012)
  • Author(s): 熊 宇,古谷優, 武田侑子,林 美加子,今里聡
  • Organizer: 第136回日本歯科保存学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-29
• [Presentation] 象牙質の構成成分および微細構造における個体差が機械的性質に及ぼす影響 (2012)
  • Author(s): 武田侑子,古谷優,石本卓也,中野貴由,林美加子
  • Organizer: 第136回日本歯科保存学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-28
• [Presentation] Chemical changes through UV strengthening of human dentin. (2012)
  • Author(s): Furuya Y, Takeda Y, Hayashi M
  • Organizer: 90th International Association for Dental Research General Session
  • Place of Presentation: Iguasu, Brazil.
  • Year and Date: 2012-06-20
• [Presentation] Best ways to treat pulpless teeth Practical strategies to prevent root fracture (2012)
  • Author(s): Hayashi M
  • Organizer: International Symposium for Adhesive Dentistry
  • Place of Presentation: Tokyo
  • Year and Date: 2012-05-19
• [Presentation] How to achieve the best oral cosmetic appearance while preserving maximum natural tissues. (2012)
  • Author(s): Hayashi M
  • Organizer: 28th American Academy of Cosmetic Dentistry
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2012-05-04
• [Presentation] How to achieve the best oral cosmetic appearance while preserving maximum natural tissues (2012)
  • Author(s): Hayashi M
  • Organizer: The 28th American Academy of Cosmetic Dentistry(
  • Place of Presentation: Washington DC
  • Invited
• [Presentation] Chemical changes through UV strengthening of human dentin (2012)
  • Author(s): Furuya Y
  • Organizer: The 90th International Association for Dental Research General Session
  • Place of Presentation: Iguasu
• [Presentation] バイオメカニクスの視点でとらえる高齢者の歯根破折 (2012)
  • Author(s): 林 美加子
  • Organizer: 第137回日本歯科保存学会
  • Place of Presentation: 広島市
  • Invited
• [Presentation] う蝕病巣におけるう蝕歯質の硬さと DIAGNOdent による評価との関係 (2011)
  • Author(s): 岩見 行晃,山本 洋子,林美加子,恵比須繁之
  • Organizer: 第135回日本歯科保存学会
  • Place of Presentation: 大阪市.
  • Year and Date: 2011-10-21
• [Presentation] 赤外線サーモグラフィを用いた歯根破折診断法の開発 (2011)
  • Author(s): 松下真美,三浦治郎,竹重文雄,岩見行晃,阪上隆英,和泉遊以,林美加子,今里聡, 恵比須繁之
  • Organizer: 第135回日本歯科保存学会
  • Place of Presentation: 大阪市.
  • Year and Date: 2011-10-20
• [Presentation] Modern caries treatments with the aim of protecting and preserving teeth (2011)
  • Author(s): Hayashi M
  • Organizer: 4th World Society of Lingual Orthodontic Congress(招待講演)
  • Place of Presentation: Osaka
• [Presentation] Analysis of strengthening mechanisms of human dentin by UV irradiation. (2011)
  • Author(s): Furuya Y, Hayashi M et.al.
  • Organizer: International Materials Congress 201
  • Place of Presentation: Seoul
• [Presentation] Analyses of stress distributions and fracture strength of pulpless teeth restored with fiber posts. (2011)
  • Author(s): Takeda Y, Hayashi M et.al.
  • Organizer: International Materials Congress 2011
  • Place of Presentation: Seoul
• [Presentation] Risk assessment of dental caries in adult patients using multiple logistic regression analysis. (2011)
  • Author(s): Hayashi M et.al.
  • Organizer: 88th European Organisation for Caries Research
  • Place of Presentation: Kaunas
• [Book] 削るう蝕・削らないう蝕 (2012)
  • Author(s): 今里聡, 林美加子, 伊藤 中
  • Publisher: クインテッセンス出版, 東京
• [Book] 削るう蝕・削らないう蝕 (2012)
  • Author(s): 今里 聡
  • Total Pages: 215
  • Publisher: クインテッセンス