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Development of virus-free generation and xeno-free culture of human gingiva-derived iPS cells

Research Project

Project/Area Number 23659896
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Prosthetic dentistry
Research InstitutionOsaka University

Principal Investigator

YATANI Hirofumi  大阪大学, 大学院・歯学研究科, 教授 (80174530)

Co-Investigator(Kenkyū-buntansha) EGUSA Hiroshi  大阪大学, 大学院・歯学研究科, 助教 (30379078)
Research Collaborator YU Guannan  大阪大学, 大学院・歯学研究科, 大学院生
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsiPS細胞 / 歯肉 / ウイルスフリー
Research Abstract

iPS cells are expected to be a powerful tool for regenerative treatments; however, problems that need to be addressed prior to clinical application include viral-vector-derived insertional mutagenesis and the use of animal-derived feeder cells for culture. We generated human gingival fibroblast (hGF)-derived iPS cells with episomal plasmid vectors. The resulting iPS cell clones could be maintained on hGF feeder cells. The virus-free and autologous culture of hGF derived-iPS cells thus represents an important step toward the future therapeutic usage of iPS cells.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (48 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 2 results) Presentation (36 results) (of which Invited: 8 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Stem cells in dentistry – Part II: Clinical applications2012

    • Author(s)
      Egusa H, Sonoyama W, Nishimura M, Atsuta I, Akiyama K
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 56 Issue: 4 Pages: 229-248

    • DOI

      10.1016/j.jpor.2012.10.001

    • NAID

      130004546661

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stem cells in dentistry – Part I: Stem cell sources2012

    • Author(s)
      Egusa H, Sonoyama W, Nishimura M, Atsuta I, Akiyama K
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 56 Issue: 3 Pages: 151-165

    • DOI

      10.1016/j.jpor.2012.06.001

    • NAID

      130004546652

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] iPS 細胞と遺伝子診断で進化する再生医療~最先端の Personalized Dentistry.2012

    • Author(s)
      江草 宏,西村一郎
    • Journal Title

      GC Circle

      Volume: 142 Pages: 4-11

    • URL

      http://www.gcdental.co.jp/clinicalconv/pdf/no142.pdf

    • Related Report
      2012 Final Research Report
  • [Journal Article] 対談:iPS細胞と遺伝子診断で進化する再生医療~最先端のPersonalized Dentistry2012

    • Author(s)
      江草 宏,西村一郎
    • Journal Title

      GC Circle

      Volume: 142 Pages: 4-11

    • Related Report
      2012 Annual Research Report
  • [Journal Article] iPS細胞と歯科2012

    • Author(s)
      江草 宏
    • Journal Title

      Clinical Calcium

      Volume: 22(1) Pages: 67-73

    • Related Report
      2011 Research-status Report
  • [Journal Article] 歯肉をiPS細胞源とする歯科医療技術の創成2011

    • Author(s)
      江草 宏、矢谷博文
    • Journal Title

      日本歯科評論

      Volume: 71 (7) Pages: 9-11

    • Related Report
      2011 Research-status Report
  • [Journal Article] 歯肉のあらたな可能性-医療応用に有望なiPS細胞源-2011

    • Author(s)
      江草 宏
    • Journal Title

      大阪大学歯学雑誌

      Volume: 56(1) Pages: 31-40

    • NAID

      10029797895

    • Related Report
      2011 Research-status Report
  • [Journal Article] Gingiva-derived iPS cells: A promising tool for dental applications2011

    • Author(s)
      江草 宏
    • Journal Title

      Proceedings of 4th Hiroshima Conference on Education and Science in Dentistry

      Volume: なし Pages: 32-36

    • Related Report
      2011 Research-status Report
  • [Presentation] Virus-free and autologous culture system for human gingiva-derived iPS cells.2013

    • Author(s)
      Yu G, Egusa H, Wang F, Yatani H.
    • Organizer
      91th IADR General Session
    • Place of Presentation
      米国 Seattle
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of gingiva derived iPS cell technologies in dental research .2013

    • Author(s)
      江草 宏
    • Organizer
      University of Washington Bioengineering Departmental Seminar
    • Place of Presentation
      米国 Seattle
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of gingiva derived iPS cell technologies in regenerative dental medicine2013

    • Author(s)
      江草 宏
    • Organizer
      International Symposium on Human Resource Development towards Global Initiative 国際シンポジウム(招待講演)
    • Place of Presentation
      Cha-am, Thailand
    • Year and Date
      2013-02-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of gingiva derived iPS cell technologies in dental research2012

    • Author(s)
      江草 宏
    • Organizer
      第60回 国際歯科研究学会 日本部会(JADR)学術大会 国際シンポジウム
    • Place of Presentation
      新潟市(招待講演)
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Application of gingiva derived iPS cells in bone biology2012

    • Author(s)
      江草 宏
    • Organizer
      The 2nd International Anatomical Sciences and Cell Biology Conference 国際シンポジウム(招待講演)
    • Place of Presentation
      Chiang Mai, Thailand
    • Year and Date
      2012-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉から作る万能細胞(iPS 細胞)が可能にする歯科医療とは2012

    • Author(s)
      江草 宏
    • Organizer
      神奈川歯科大学学会 研究談話会講演
    • Place of Presentation
      横須賀市(招待講演)
    • Year and Date
      2012-11-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉由来 iPS 細胞の歯科医学への応用2012

    • Author(s)
      江草 宏
    • Organizer
      第54回 歯科基礎医学会学術大会シンポジウム
    • Place of Presentation
      郡山市(招待講演)
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] iPS 細胞の骨生物学への応用2012

    • Author(s)
      江草 宏
    • Organizer
      硬組織再生生物学会 第21回学術大会
    • Place of Presentation
      名古屋市(招待講演)
    • Year and Date
      2012-08-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications2012

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部・大学院歯学研究科「口の難病から挑むライフ・イノベーション」日本・韓国国際シンポジウム(招待講演)
    • Place of Presentation
      大阪府吹田市
    • Year and Date
      2012-07-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications2012

    • Author(s)
      江草 宏
    • Organizer
      The 90th IADR General Session 国際シンポジウム
    • Place of Presentation
      Iguacu Falls,Brazil(招待講演)
    • Year and Date
      2012-06-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉由来 iPS 細胞の口腔先端医学への応用2012

    • Author(s)
      江草 宏
    • Organizer
      口腔先端応用医科学研究会 第4回学術会議(招待口演)
    • Place of Presentation
      東京
    • Year and Date
      2012-01-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉を iPS 細胞源とする歯科医療技術の創成2012

    • Author(s)
      江草 宏
    • Organizer
      日本歯科医学会 第28回「歯科医学を中心とした総合的な研究を推進する集い」(指定演者)
    • Place of Presentation
      東京
    • Year and Date
      2012-01-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉をiPS細胞源とする歯科医療技術の創成2012

    • Author(s)
      江草 宏
    • Organizer
      日本歯科医学会 第28回「歯科医学を中心とした総合的な研究を推進する集い」(招待講演)
    • Place of Presentation
      東京都
    • Related Report
      2011 Research-status Report
  • [Presentation] 歯肉のあらたな可能性-歯肉から万能細胞(iPS 細胞)を作る-2011

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部創立 60 周年記念 オープンフェスタ in Suita
    • Place of Presentation
      大阪府吹田市(招待講演)
    • Year and Date
      2011-10-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯茎から作れる万能細胞~iPS細胞で夢の再生医療へ~.2011

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部60 周年記念事業 第9回市民フォーラム
    • Place of Presentation
      大阪市(招待講演)
    • Year and Date
      2011-10-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications2011

    • Author(s)
      江草 宏
    • Organizer
      The 4th Hiroshima Conference on Education and Science in Dentistry 国際シンポジウム(招待講演)
    • Place of Presentation
      広島市
    • Year and Date
      2011-10-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉のあらたな可能性-医療応用に有望な iPS 細胞源-2011

    • Author(s)
      江草 宏
    • Organizer
      日本口腔インプラント学会 第41回学術大会
    • Place of Presentation
      名古屋市(招待講演)
    • Year and Date
      2011-09-17
    • Related Report
      2012 Final Research Report
  • [Presentation] 口腔粘膜歯肉を用いた iPS 細胞の樹立および骨生物学への応用2011

    • Author(s)
      江草 宏
    • Organizer
      日本骨代謝学会 第29回学術集会 若手シンポジウム
    • Place of Presentation
      大阪市(招待講演)
    • Year and Date
      2011-07-30
    • Related Report
      2012 Final Research Report
  • [Presentation] The Impact of Gingival Fibroblast-Derived iPS Cells in Dentistry .2011

    • Author(s)
      江草 宏
    • Organizer
      The 3rd Thailand International Conference on Oral Biology 国際シンポジウム(招待講演)
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2011-05-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉を iPS 細胞源とする補綴歯科治療の可能性2011

    • Author(s)
      江草 宏
    • Organizer
      日本補綴歯科学会 第120回学術大会シンポジウム
    • Place of Presentation
      広島市(招待講演)
    • Year and Date
      2011-05-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯肉をiPS細胞源とする補綴歯科治療の可能性2011

    • Author(s)
      江草 宏
    • Organizer
      日本補綴歯科学会 第120回学術大会・シンポジウム(招待講演)
    • Place of Presentation
      広島市
    • Related Report
      2011 Research-status Report
  • [Presentation] The Impact of Gingival Fibroblast-Derived iPS Cells in Dentistry2011

    • Author(s)
      江草 宏
    • Organizer
      The 3rd Thailand International Conference on Oral Biology(招待講演)
    • Place of Presentation
      タイ王国,バンコク
    • Related Report
      2011 Research-status Report
  • [Presentation] 口腔粘膜歯肉を用いたiPS細胞の樹立および骨生物学への応用2011

    • Author(s)
      江草 宏
    • Organizer
      日本骨代謝学会 第29回学術集会・シンポジウム(招待講演)
    • Place of Presentation
      大阪市
    • Related Report
      2011 Research-status Report
  • [Presentation] 歯肉のあらたな可能性-医療応用に有望なiPS細胞源-2011

    • Author(s)
      江草 宏
    • Organizer
      日本口腔インプラント学会 第41回学術大会(招待講演)
    • Place of Presentation
      名古屋市
    • Related Report
      2011 Research-status Report
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications2011

    • Author(s)
      江草 宏
    • Organizer
      The 4th Hiroshima Conference on Education and Science in Dentistry・シンポジウム(招待講演)
    • Place of Presentation
      広島市
    • Related Report
      2011 Research-status Report
  • [Presentation] 歯茎から作れる万能細胞~iPS細胞で夢の再生医療へ~2011

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部60周年記念事業 第9回市民フォーラム「再生歯科医療」(招待講演)
    • Place of Presentation
      大阪市
    • Related Report
      2011 Research-status Report
  • [Presentation] 歯肉のあらたな可能性-歯肉から万能細胞(iPS細胞)を作る-2011

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部創立60周年記念 オープンフェスタ in Suita(招待講演)
    • Place of Presentation
      吹田市
    • Related Report
      2011 Research-status Report
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications

    • Author(s)
      江草 宏
    • Organizer
      The 90th IADR General Session 国際シンポジウム
    • Place of Presentation
      Iguacu Falls, Brazil
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部・大学院歯学研究科「口の難病から挑むライフ・イノベーション」日本・韓国国際シンポジウム
    • Place of Presentation
      大阪府吹田市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] iPS細胞の骨生物学への応用

    • Author(s)
      江草 宏
    • Organizer
      硬組織再生生物学会 第21回学術大会 特別講演
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 歯肉由来iPS細胞の歯科医学への応用

    • Author(s)
      江草 宏
    • Organizer
      第54回 歯科基礎医学会学術大会 シンポジウム
    • Place of Presentation
      郡山市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 歯肉から作る万能細胞(iPS細胞)が可能にする歯科医療とは

    • Author(s)
      江草 宏
    • Organizer
      神奈川歯科大学学会 研究談話会講演
    • Place of Presentation
      横須賀市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Application of gingiva-derived iPS cells in bone biology

    • Author(s)
      江草 宏
    • Organizer
      The 2nd International Anatomical Sciences and Cell Biology Conference 国際シンポジウム
    • Place of Presentation
      Chiang Mai, Thailand
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Application of gingiva-derived iPS cell technologies in dental research

    • Author(s)
      江草 宏
    • Organizer
      第60回 国際歯科研究学会 日本部会(JADR)学術大会 国際シンポジウム
    • Place of Presentation
      新潟市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Application of gingiva-derived iPS cell technologies in regenerative dental medicine

    • Author(s)
      江草 宏
    • Organizer
      International Symposium on Human Resource Development towards Global Initiative 国際シンポジウム
    • Place of Presentation
      Cha-am, Thailand
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Virus-free and autologous culture system for human gingiva-derived iPS cells

    • Author(s)
      Yu G, Egusa H, Wang F, Yatani H
    • Organizer
      91th IADR General Session
    • Place of Presentation
      Seattle, USA
    • Related Report
      2012 Annual Research Report
  • [Book] The impact of gingival fibroblast-derived iPS cells in dentistry: in Interface Oral health Science 2011, Eds: Sasaki K, Suzuki O, Takahashi N2012

    • Author(s)
      江草 宏
    • Total Pages
      5
    • Publisher
      Springer, Tokyo Berlin Heidelberg New York
    • Related Report
      2011 Research-status Report
  • [Remarks] (1) 毎日新聞:夢を現実に 続く挑戦-歯ぐき 捨てずに生かす・iPS 細胞活用(2011年11月8日)

    • Related Report
      2012 Final Research Report
  • [Remarks] (1) 江草 宏.国際歯科研究学会(IADR)Distinguished Scientist Award (Young Investigator Award),2012年6月 20日

    • Related Report
      2012 Final Research Report
  • [Remarks] (2) 江草 宏.大阪大学功績賞,2011年8月1日

    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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