Development of virus-free generation and xeno-free culture of human gingiva-derived iPS cells
| Project/Area Number |
23659896
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Prosthetic dentistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
YATANI Hirofumi 大阪大学, 大学院・歯学研究科, 教授 (80174530)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
EGUSA Hiroshi 大阪大学, 大学院・歯学研究科, 助教 (30379078)
|
|---|
| Research Collaborator |
YU Guannan 大阪大学, 大学院・歯学研究科, 大学院生
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | iPS細胞 / 歯肉 / ウイルスフリー |
|---|
| Research Abstract |
iPS cells are expected to be a powerful tool for regenerative treatments; however, problems that need to be addressed prior to clinical application include viral-vector-derived insertional mutagenesis and the use of animal-derived feeder cells for culture. We generated human gingival fibroblast (hGF)-derived iPS cells with episomal plasmid vectors. The resulting iPS cell clones could be maintained on hGF feeder cells. The virus-free and autologous culture of hGF derived-iPS cells thus represents an important step toward the future therapeutic usage of iPS cells.
|
Report
(3 results)
Research Products
(48 results)
