| Project/Area Number |
23659913
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
IZUMI Kenji 新潟大学, 医歯学系, 教授 (80242436)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 口腔粘膜 / 上皮細胞 / 再生医療 / 老化 / Akt/mTOR / 低酸素 / エイジング / PPARgamma / アゴニスト / PTEN / PPARγ / アンチエイジング / PI3K/Aktシグナリング |
|---|
| Research Abstract |
Decreasing of WST-8 activity and increasing of GLUT-1 expression suggested that keratinocytes metabolism were shift oxidative metabolism to anaerobic glycolysis in hypoxic culture. Cell cycle profile shows G0/G1 arrest in hypoxic culture. In addition, we assessed p21 expression concomitant with cell cycle analysis because "cycling cells" in G1 phase do not express p21. p21 expression were lower in hypoxic culture suggested that greater number of G1 cells existed in hypoxic culture. Thus, significant higher colony forming efficiency (CFE) shown in cells under hypoxic conditions was due to a higher proportion of cycling cells. Finally, using a tissue-engineered oral mucosa (TEOM) equivalent, we investigated the effect of hypoxia on the epithelial structure for regular and long-term cultivation. The histological examinations showed that the epithelial homeostasis of the TEOM was better sustained in hypoxic condition.
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