| Project/Area Number |
23659914
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nagoya University |
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Principal Investigator |
UEDA Minoru 名古屋大学, 医学系研究科, 教授 (00151803)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Akihito 名古屋大学, 大学院・医学系研究科, 准教授 (50244083)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ヒト歯髄幹細胞 / 培養上清 / 脊髄損傷 / 難治性中枢神経系疾患 / 神経再生 / 歯髄幹細胞 / ミクログリア / 抗炎症 |
|---|
| Research Abstract |
We found that conditioned medium (CM) from stem cells derived from human exfoliated deciduous teeth (SHED), administered intrathecally into the severely injured adult rat SC, resulted in marked functional recovery. SHED-CM treatment inhibited SCI-induced apoptosis, preserved neural fibers and myelin sheaths, and promoted descending serotonergic raphespinal axon growth. Importantly, these pathophysiological recoveries were supported by a remarkable immunoregulatory function of SHED-CM, which converted the pro-inflammatory SCI conditions to a tissue repair/regenerating platform by modulating the microglia/macrophage phenotype. SHED-CM directly induced IL-10-producing M2 microglia synergistically with a major glial-scar extracellular matrix component, chondroitin sulphate proteoglycan, in vitro. Thus, SHED-CM may enable new stem-cell-based regenerative therapies for SCI that do not involve cell transplantation.
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