We elucidated osteogenic capacity of CD90-posidive cells and side population (SP) cells derived from bone marrow stromal cells to establish the hard tissue regeneration therapy. Most of bone marrow stromal cells expressed CD90, one of mesenchymal stem cell markers, and contained 0.5% SP cells. CD90-positive cells and SP cells could differentiate osteoblastic cells in in vitro and in vivo. However, we could not clarify specific genes of osteoprogenitor cells.

Research Project

Project/Area Number	23659926
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Dental engineering/Regenerative dentistry
Research Institution	Matsumoto Dental University
Principal Investigator	NAKAMURA Hiroaki 松本歯科大学, 歯学部, 教授 (50227930)
Co-Investigator(Kenkyū-buntansha)	HOSOYA Akihiro 松本歯科大学, 歯学部, 講師 (70350824) YUKITA Akira 松本歯科大学, 歯学部, 助教 (80401214) NINOMIYA Tadashi 松本歯科大学, 総合歯科医学研究所, 講師 (00360222)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	骨原性細胞 / 骨髄間葉系細胞 / CD90 / Side population / 骨髄間様系細胞 / Hoechst 33342 / side population / 骨髄間質細胞
Research Abstract	We elucidated osteogenic capacity of CD90-posidive cells and side population (SP) cells derived from bone marrow stromal cells to establish the hard tissue regeneration therapy. Most of bone marrow stromal cells expressed CD90, one of mesenchymal stem cell markers, and contained 0.5% SP cells. CD90-positive cells and SP cells could differentiate osteoblastic cells in in vitro and in vivo. However, we could not clarify specific genes of osteoprogenitor cells.