Analysis of cleft palate signal by using CASK knockout mouse

• Project/Area Number: 23659935
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: University of Tsukuba
• Principal Investigator: YANAGAWA Toru 筑波大学, 医学医療系, 准教授 (10312852)
• Co-Investigator(Kenkyū-buntansha): TABUCHI Katsuhiko 信州大学, 医学部, 教授 (20546767)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口腔外科学一般 / CASK / 口蓋裂 / ノックアウトマウス

Research Abstract

We hypothesize that CASK signal affects cleft palate formation because CASK knock out mouse has cleft palate. To clarify this signal we identified the candidate molecules that associate with CASK by using Q-TOF LC/MS. We selected Neurexin as a signal transduction molecule, then adherent domain and interaction between molecules was analyzed. In addition CASK flox/flox mouse was analyzed and estimated the role of CASK in cleft palate formation. On the other hand we developed a new method which evaluate morphological similarity to use mouse facial deformity.

Research Products

• [Journal Article] Presurgical nasoalveolar molding orthopedic treatment improves the outcome of primary cheiloplasty of unilateral complete cleft lip and palate, as assessed by naris morphology and cleft gap (2012)
  • Author(s): Sasaki H, Togashi S, Karube R, Yanagawa T, Nakane S, Tabuchi K, Ishibashi N, Shinya Y, Ito H, Yamagata K, Onizawa K, Adachi K, Sekido M, Bukawa H
  • Journal Title: J Craniofac Surg Volume: 23(6) Pages: 1596-601
  • Peer Reviewed
• [Journal Article] A novel method for evaluating postsurgical results of unilateral cleft lip and palate with theuse of Hausdorff distance. (2012)
  • Author(s): Toru Yanagawa, Shizuo Nakane, et.al
  • Journal Title: Oral Surg OralMed Oral Pathol Oral Radiol Volume: Vol.114 Issue: 6 Pages: 704-711
  • DOI: 10.1016/j.oooo.2012.01.042
  • Peer Reviewed
• [Journal Article] Presurgical nasoalveolar molding orthopedic treatment improves the outcome of primary cheiloplasty of unilateral complete cleft lip and palate, as assessed by naris morphology and cleft gap. (2012)
  • Author(s): Toru Yanagawa, Shizuo Nakane, et.al.
  • Journal Title: Journal of Craniofacial Surgery Volume: Vol. 23 Issue: 6 Pages: 1596-1601
  • DOI: 10.1097/scs.0b013e31825196dc
  • Peer Reviewed
• [Journal Article] A novel method for evaluating postsurgical results of unilateral cleft lip and palate with the use of Hausdorff distance: Presurgical orthopedic treatment improves nasal symmetry after primary cheiloplasty (2012)
  • Author(s): Karube R
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Volume: in press
  • Peer Reviewed
• [Journal Article] Presurgical nasoalveolar molding orthopedic treatment improves the outcome of primary cheiloplasty of unilateral complete cleft lip and palate, as assessed by naris morphology and cleft gap (2012)
  • Author(s): Sasaki H
  • Journal Title: J Craniofac Surg Volume: in press
  • Peer Reviewed