Minimal reprogramming of cultured MSC based on DNA methylation analysis
| Project/Area Number |
23659938
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HOSHI Kazuto 東京大学, 医学部附属病院, 准教授 (30344451)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATO Tsuyoshi 東京大学, 医学部附属病院, 教授 (90171454)
MORI Yoshiyuki 東京大学, 医学部附属病院, 准教授 (70251296)
HUJIHARA Yuko 東京大学, 医学(系)研究科(研究院), 特任助教 (50466744)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 再生医療 / 間葉系幹細胞 / DNAメチル化 / 骨再生医療 / ＭＳＣ / メチル化 / リプログラミング / ＤＮＡメチル化 |
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| Research Abstract |
The purpose of this research is to establish safe and reliable technology for regenerative medicine using the cultured mesenchymal stem cells (MSCs), which are reset by minimum reprogramming. In this research, we analyzed DNA methylation of cultured rat MSCs genome-widely, and identified the variations of methylation with cell deterioration. By using stimulus to DNA demethylation,we examined a method to improve the state of the methylation, and applied the method to a bone regeneration model in pig.
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Report
(3 results)
Research Products
(44 results)
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[Journal Article] Nanog promotes osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2 by modulating bone morphogenetic protein(BMP) signaling.2013
Author(s)
Ogasawara T, Ohba S, Yano F, Kawaguchi H, Chung UI, Saito T, Yonehara Y, Nakatsuka T, Mori Y, Takato T, and Hoshi K
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Journal Title
J Cell Physiol
Volume: (in press)
Issue: 1
Pages: 163-71
DOI
Related Report
Peer Reviewed
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