Expression and function of secreted microRNAs in oral cancer

• Project/Area Number: 23659947
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: HAMAKAWA Hiroyuki 愛媛大学, 大学院・医学系研究科, 教授 (20127905)
• Co-Investigator(Kenkyū-buntansha): NAKASHIRO Koichi 愛媛大学, 大学院・医学系研究科, 准教授 (90314880)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 口腔癌 / microRNA / エクソソーム

Research Abstract

In this study, we attempted to explore specific microRNAs (miRNAs) in serum and saliva derived from patients with oral cancer. Specifically 17 increased miRNAs and 15 undetectable miRNAs in serum from oral cancer patients were commonly identified. Among these miRNAs, hsa-miR-181b was markedly elevated in not only serum but also saliva from oral cancer patients. These miRNAs may be useful for detection of oral cancer.

Research Products

• [Journal Article] Targeting Aurora kinase A suppresses the growth of human oral squamous cell carcinoma cells in vitro and in vivo (2013)
  • Author(s): Tanaka H, Nakashiro K, Iwamoto K, Tokuzen N, Fujita Y, Shirakawa R, Oka R,Goda H, Hamakawa H
  • Journal Title: Oral Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Prognostic impact of expression of bcl-2 and bax genes in circulating immune cells derived from patients with head and neck carcinoma (2013)
  • Author(s): Tano T, Okamoto M, Kan S, Nakashiro K, Shimodaira S, Koido S, Homma S, Sato M,Fujita T, Kawakami Y, Hamakawa H
  • Journal Title: Neoplasia Volume: 15(3) Pages: 305-14
  • Peer Reviewed
• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma (2012)
  • Author(s): Goda H, Nakashiro K, Oka R, Tanaka H, Wakisaka H, Hato N, Hyodo M, Hamakawa H
  • Journal Title: Oral Oncol Volume: 48(10) Pages: 958-63
  • Peer Reviewed
• [Journal Article] Premetastatic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes (2012)
  • Author(s): Mayorca-Guiliani AE, Yano H, Nakashiro K, Hamakawa H, Tanaka J
  • Journal Title: Oral Oncol Volume: 48(8) Pages: 663-70
  • Peer Reviewed
• [Journal Article] Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines (2012)
  • Author(s): Tano T, Okamoto M, Kan S, Nakashiro K, Shimodaira S, Yamashita N, Kawakami Y, Hamakawa H
  • Journal Title: Oral Oncol Volume: 48(8) Pages: 678-85
  • Peer Reviewed
• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma (2012)
  • Author(s): Goda H
  • Journal Title: Oral Oncology Volume: 48 Issue: 10 Pages: 958-63
  • DOI: 10.1016/j.oraloncology.2012.03.026
  • Peer Reviewed
• [Journal Article] Premetastatic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes (2012)
  • Author(s): Mayorca-Guiliani AE
  • Journal Title: Oral Oncology Volume: 48 Issue: 8 Pages: 663670-663670
  • DOI: 10.1016/j.oraloncology.2012.02.007
  • Peer Reviewed
• [Journal Article] Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines (2012)
  • Author(s): Tano T
  • Journal Title: Oral Oncology Volume: 48 Issue: 8 Pages: 678-85
  • DOI: 10.1016/j.oraloncology.2012.02.005
  • Peer Reviewed
• [Journal Article] Premetastic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes (2012)
  • Author(s): Mayorca-Guilliani AE, Yano H, Nakashiro K, Hamakawa H, Tanaka J
  • Journal Title: Oral Oncology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines (2012)
  • Author(s): Tano T, Okamoto M, Kan S, Nakashiro K, Shimodaira S, Yamashita N, Kawakami Y, Hamakawa H
  • Journal Title: Oral Oncology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Human FAT1 cadherin controls cell migration and invasion of oral squamous cell carcinoma through the localization of β-catenin (2011)
  • Author(s): Nishikawa Y, Miyazaki T, Nakashiro K, Yamagata H, Isokane M, Goda H, Tanaka H, Oka R, Hamakawa H
  • Journal Title: Oncol Rep Volume: 26(3) Pages: 587-92
  • Peer Reviewed
• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2011)
  • Author(s): Zhang T, Hamada K, Hyodo M, Itoh H, Tani K, Goda H, Nakashiro K, Hamakawa H
  • Journal Title: Oncol Rep Volume: 25(3) Pages: 795-802
  • Peer Reviewed
• [Journal Article] Novel therapeutic strategies for malignant salivary gland tumors: lesson learned from breast cancer (2011)
  • Author(s): Murase R, Sumida T, Ishikawa A, Murase R, McAllister SD, Hamakawa H, Desprez PY
  • Journal Title: International Journal of Otolaryngology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Human FAT1 cadherin controls cell migration and invasion of oral squamous cell carcinoma through the localization of beta-catenin (2011)
  • Author(s): Nishikawa, Y.
  • Journal Title: Oncology Reports Volume: 26(3) Pages: 587-592
  • DOI: 10.3892/or.2011.1324
  • Peer Reviewed
• [Presentation] ヒト口腔癌細胞における microRNA-629* の増殖抑制効果 (2012)
  • Author(s): 岩本和樹、中城公一、田中宏史、岡 亮太、浜川裕之
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] ヒト口腔癌細胞における microRNA-629 の機能解析 (2012)
  • Author(s): 岩本和樹、中城公一、田中宏史、徳善紀彦、岡 亮太、浜川裕之
  • Organizer: 第4回日本 RNAi 研究会
  • Place of Presentation: 広島
• [Presentation] MicroRNA-361-p に対する LNA アンチセンスオリゴヌクレオチドの機能阻害効果の検討 (2012)
  • Author(s): 田中宏史、中城公一、浜川裕之
  • Organizer: 第4回日本 RNAi 研究会
  • Place of Presentation: 広島
• [Presentation] ヒト口腔癌細胞における癌遺伝子様microRNA の同定 (2011)
  • Author(s): 田中宏史、中城公一、岡亮太、浜川裕之
  • Organizer: 第10回中国四国口腔癌研究会
  • Place of Presentation: 松山
  • Year and Date: 2011-11-25
• [Presentation] ヒト口腔癌細胞における癌遺伝子様 microRNA の探索 (2011)
  • Author(s): 田中宏史、中城公一、岡亮太、浜川裕之
  • Organizer: 第3回日本RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 2011-08-26
• [Presentation] MicroRNA-361-3p regulates the expression of OSR2 and functions as oncogenic microRNA in human oral cancer cells (2011)
  • Author(s): Tanaka H, Nakashiro K, Oka R, Nishikawa Y, Goda H, Hamakawa H
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] MicroRNA-361-3p functions as an oncogenic microRNA in human oral cancer cells (2011)
  • Author(s): Tanaka H, Nakashiro K, Oka R, Nishikawa Y, Hamakawa H
  • Organizer: 102nd American Association for Cancer Research Annual Meeting
  • Place of Presentation: Orlando, FL
• [Presentation] 口腔癌 SNNS の検証と新戦略 (2011)
  • Author(s): 浜川裕之
  • Organizer: 第 13 回 SNNS 研究会学術集会(招待講演)
  • Place of Presentation: 浜離宮朝日ホール・東京
• [Patent(Industrial Property Rights)] アンドロゲン受容体遺伝子に特異的な siRNA (2012)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Acquisition Date: 2012-04-06
• [Patent(Industrial Property Rights)] 頭頸部癌の腫瘍マーカー (2012)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Acquisition Date: 2012-04-13
• [Patent(Industrial Property Rights)] ADAT1 遺伝子に特異的な siRNA (2012)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Acquisition Date: 2012-10-12
• [Patent(Industrial Property Rights)] マイクロ RNA 又はその発現系を含む組成物 (2011)
  • Inventor(s): 中城公一、浜川裕之、田中宏史
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Number: 2011-113348
  • Filing Date: 2011-05-20
• [Patent(Industrial Property Rights)] マイクロＲＮＡ又はその発現系を含む組成物 (2011)
  • Inventor(s): 中城公一、浜川裕之、田中宏史
  • Industrial Property Rights Holder: 愛媛大学
  • Industrial Property Number: 2011-113348
  • Filing Date: 2011