The regenerative therapy of the jaw by auto-transplantation of adipose-derived stem cells

• Project/Area Number: 23659956
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: SHINTANI Satoru 昭和大学, 歯学部, 教授 (80294429)
• Co-Investigator(Kenkyū-buntansha): KONDO Seiji 昭和大学, 歯学部, 准教授 (10432634) ; MUKUDAI Yoshiki 昭和大学, 歯学部, 助教 (50325099)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 脂肪幹細胞 / 骨芽細胞 / 軟骨細胞 / 低侵襲性医療 / 骨再生 / 骨 / 軟骨 / 自家移植

Research Abstract

In the present study, we revealed as followed.
1) The advantages of chemical and mechanicalstress and a cobimation of those on human adipose-derived stem cells (ADSCs) have been revealed. 2) By the transplanation of the diffentiated ADSCs to nude mice, the histochmical and mmolecular-biological investigations of the transplants, wich was succesfully differntiated into bone tissue, have been carried out. 3) It has been suggested that the therapy might contribute the nobel and advantageous regenerative medicine for bone defect of the jaws, since the study was not utilize gene transfection like iPS cells.

Research Products

• [Journal Article] Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro (2014)
  • Author(s): Mukudai, Y, Kondo, S., Koyama, T., Li C., Banka, S., Kogure, A., Yazawa, K., Shintani, S
  • Journal Title: BMC Complement Altern Med Volume: 14 Pages: 29-29
  • Peer Reviewed
• [Journal Article] Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma (2014)
  • Author(s): Kondo, S., Mukudai, Y., Soga, D., Nishida, T., Takigawa, M., Shirota
  • Journal Title: Anticancer Res Volume: 34 Pages: 671-677
  • Peer Reviewed
• [Journal Article] Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro. (2014)
  • Author(s): *Mukudai, Y, Kondo, S., Koyama, T., Li C., Banka, S., Kogure, A., Yazawa, K., Shintani, S.
  • Journal Title: BMC Complement Altern Med. Volume: 14 Issue: 1 Pages: 29-29
  • DOI: 10.1186/1472-6882-14-29
  • Peer Reviewed / Open Access
• [Journal Article] The root bark extracts of Juncus effusuc and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death induced by cis-platinum (II) diammine dichloride (2013)
  • Author(s): Mukudai, Y., Kondo, S., Shiogama, S., Koyama, T., Li, C., Yazawa, K., Shintani, S
  • Journal Title: Oncol Rep Volume: 30 Pages: 2665-71
• [Journal Article] The root bark extracts of Juncus effusuc and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death induced by cis-platinum (II) diammine dichloride (2013)
  • Author(s): Mukudai, Y., Kondo, S., Shiogama, S., Koyama, T., Li, C., Yazawa, K., Shintani, S
  • Journal Title: Oncol Rep. Volume: 30 Pages: 2665-2671
  • Peer Reviewed
• [Journal Article] A potential anticancer activity of extracts derived from the roots of Scutellaria baicalensis on humanoral squamous cell carcinoma cells (2013)
  • Author(s): Sato D, Yazawa K, Kondo S, Mukudai Y, Li C,Kamatani T, Katsuta H, Yoshihama Y,Shirota T,Shintani S
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Issue: 1 Pages: 105-111
  • DOI: 10.3892/mco.2012.14
  • Peer Reviewed
• [Journal Article] A combination of chemical and mechanical stimuli enhances not only osteo-but also chondro-differentiation in adipose-derived stem cells (2012)
  • Author(s): Banka, S., Mukudai, Y., Yoshihama, Y., Shirota, T., Kondo, S., Shintani, S
  • Journal Title: J. Oral Biosci Volume: 54(7) Pages: 188-195
• [Journal Article] The root bark of Paeonia moutan is a potential anticancer agent in human oral squamous cell carcinoma cells (2012)
  • Author(s): Li C, Yazawa K, Kondo S, Mukudai Y, Sato D, Kurihara Y, Kamatani T, Shintani S
  • Journal Title: Anticancer research Volume: 32 Pages: 2625-2630
  • Peer Reviewed
• [Journal Article] A combination of chemical and mechanical stimuli enhances not only osteo-but also chondro-differentiation in adipose-derived cells (2012)
  • Author(s): Banka S, Mukudai Y, Yoshihama Y, Shirota T, Kondo S, Shintani S
  • Journal Title: Journal of oral Biosciences Volume: 54 Pages: 188-195
  • Peer Reviewed
• [Presentation] A combination of chemical and mechanical stimuli synergistically enhances osteo-and chondro-differentation on adipose-derived stem cells (2012)
  • Author(s): Shintani S, Banka S, Mukudai Y, Kondo S
  • Organizer: 21 Congress of the European Association for Cranio-Maxillofacial Surgery
  • Place of Presentation: Dubrovnik, Croatia
• [Presentation] Mandibular reconstruction using an autoclave autogenous bone and Iliac particulate cancellous bone and marrow (PCBM) (2012)
  • Author(s): Shintani S, Kutsuna T, Yoshiba S, Kurihara Y, Yoshizawa Y, Yoshihama Y, Kondo S, Shirota T
  • Organizer: Asian ACOMS
  • Place of Presentation: Bali, Indonesia
• [Presentation] 脂肪幹細胞(ADSC)から骨形成細胞への分化に対する化学的、機械的刺激の影響
  • Author(s): 番家政香, 椋代義樹, 近藤誠二, 新谷悟
  • Organizer: 第２８回日本骨代謝学会
  • Place of Presentation: 大阪