Investigation about dental caries vaccine using human-type anti-GTF antibody
| Project/Area Number |
23659968
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUJIWARA Taku 長崎大学, 医歯(薬)学総合研究科, 教授 (00228975)
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| Co-Investigator(Renkei-kenkyūsha) |
星野 倫範 長崎大学, 医歯薬学総合研究科小児歯科学分野, 准教授 (00359960)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | う蝕 / ワクチン / GTF / イネ / ＧＴＦ |
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| Research Abstract |
Cell associated glucosyltransferases (CA-GTF) were purified from Streptococcus mutans strain MT8148. Antisera against recombinant catalytic region (CAT) and glucan binding domain (GBD), which are functional domains of GTF, were generated. Both anti-sera exhibited low inhibitory effect on glucan synthesis of GTF. These results indicatied neither CAT and GBD is not suitable for the target of vaccination. In silico prediction by the Parker method showed variable region (VR) which located N-terminus, possessed good antigenicity. DNA vaccine carrying VR was constructed. Mice antisera, immunized with the GTFB-VR DNA vaccine, exhibited reactivity with GTFB and comparable inhibitory activity of glucan synthesis as native anti-GTF serum. Since several trails to generate monoclonal antibody, which produce this antibody, were performed, no good clone was obtained. Therefore, no farther progress was performed.
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Report
(4 results)
Research Products
(9 results)
