Establishment of screening system for novel therapeutics for periodontal disease by using low molecule compound

• Project/Area Number: 23659980
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Tokyo University of Science
• Principal Investigator: SAITO Masahiro 東京理科大学, 基礎工学部, 准教授 (40215562)
• Co-Investigator(Kenkyū-buntansha): TSUJI Takashi 東京理科大学, 総合研究機構, 准教授 (50339131) ; YAMADA Satoru 大阪大学, 大学院・歯学研究科 (40359849)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯周免疫機能学 / 再生 / 歯根膜 / マイクロフィブリル / 創傷治癒誘導 / 低分子化合物 / 弾性線維 / 結合組織 / マルファン病症候群 / 創薬開発 / 弾性繊維 / マルファン病

Research Abstract

We have shown that regeneration of periodontal tissue is required for reorganization of microfibril. To develop a novel therapeutics for periodontal disease, we attempt to develop low molecular compoundto stimulate expression of ADAMTSL6・in which promotion of microfibril assembly. To achieve these purposes, we demonstrated that generation of cell possessing EGFP reporter under control by ADAMTSL6・promoter.

Research Products

• [Journal Article] Dpysl4 is involved in tooth germ morphogenesis through growth regulation, polarization and differentiation of dental epithelial cells. (2013)
  • Author(s): Yasukawa M, Ishida K, Yuge Y,Hanaoka M, Minami Y, Ogawa M, Sasaki T, Saito M, Tsuji T
  • Journal Title: Int J Biol Sci Volume: 9(4) Pages: 382-90
• [Journal Article] Roles of Heparan Sulfate Sulfation in Dentinogenesis (2012)
  • Author(s): Hayano, S., Kurosaka, H., Yanagita, T., Kalus , I. , Milz, F., Ishihara, Y., Nurul, Islam, M., Kawanabe, N., Saito, M.,Kamioki, H., Adachi, T., Dierks, T. and Yamashiro, T.*
  • Journal Title: J Biol Chem Volume: 287(15) Pages: 12217-12229
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation. (2012)
  • Author(s): Arakaki, M., Ishikawa, M., Nakamura, T., Iwamoto, T., Yamada, A., Fukumoto, E., Saito, M., Otsu, K., Harada, H., Yamada, Y. and Fukumoto, S.*
  • Journal Title: J Biol Chem Volume: 287(13) Pages: 10590-1060
• [Journal Article] Roles of Heparan Sulfate Sulfation in Dentinogenesis (2012)
  • Author(s): Hayano S, Kurosaka H, Yanagita T, Kalus I, Milz F, Ishihara Y, et al
  • Journal Title: J Biol Chem Volume: (印刷中) Issue: 15 Pages: 12217-12229
  • DOI: 10.1074/jbc.m111.332924
  • Peer Reviewed
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287(13) Issue: 13 Pages: 10590-601
  • DOI: 10.1074/jbc.m111.285874
  • Peer Reviewed
• [Journal Article] Extracellular matrix administration as a potential therapeutic strategy for periodontal ligament regeneration (2012)
  • Author(s): M.Saito and T.Tsuji
  • Journal Title: Expert Opin Biol Ther Volume: 12(3) Issue: 3 Pages: 299-309
  • DOI: 10.1517/14712598.2012.655267
  • Peer Reviewed
• [Journal Article] ADAMTSL6Lβrescues fibrillin-1 microfibril disorder in Marfan syndrome mouse model through the promotion of fibrillin-1 assembly. (2011)
  • Author(s): Saito, M.,*Kurokawa, M., Oda, M., Oshima, M., Tsutsui, K., Kosaka, K., Nakao, K., Ogawa, M., Manabe, R., Suda, N., Ganjargal, G., Hada, Y., Noguchi, T., Teranaka, T., Sekiguchi, K., Yoneda, T. and Tsuji, T
  • Journal Title: J Biol Chem Volume: 286(44) Pages: 38602-38613
• [Journal Article] Functional tooth regeneration using a bioengineered tooth unit as a mature organ replacement regenerative therapy (2011)
  • Author(s): M.Oshima, et al
  • Journal Title: PLoS ONE Volume: 6(7) Issue: 7 Pages: 10590-10601
  • DOI: 10.1371/journal.pone.0021531
  • Peer Reviewed
• [Journal Article] Induction of enamel matrix protein expression in an ameloblast cell line co-cultured with a mesenchymal cell line in vitro. (2011)
  • Author(s): Matsumoto, A., Harada, H., Saito, M. and Taniguchi, A.*
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 47(1) Pages: 39-44
• [Journal Article] ADAMTSL6&β rescues fibrillin-1 microfibril disorder in Marfan syndrome mouse model through the promotion of fibrillin-1 assembly (2011)
  • Author(s): M.Saito et, al
  • Journal Title: J Biol Chem Volume: 286(44) Issue: 44 Pages: 38602-38613
  • DOI: 10.1074/jbc.m111.243451
  • Peer Reviewed
• [Journal Article] Characterization of immortalized mouse dental follicle cell (2005)
  • Author(s): Yokoi, T., Saito, M.,* Nishida, E., Kosaka, K, Tsubakimoto, T. and Teranaka, T
  • Journal Title: Bull Kanagawa Dent Col Volume: 33(1) Pages: 136-140
• [Journal Article] Molecular mechanisms of Periodontal development, and its prospect of regenerative medicine for periodontium (2005)
  • Author(s): Saito, M.,* Tsunoda, A., Yokoi, T., Hattori, S., Tsubakimoto, T., Nishida, E., Kousaka, K., Toyoda, M., Sato, S., Noguchi, T. and Teranaka, T
  • Journal Title: Bull Kanagawa Dent Col Volume: 33(1) Pages: 31-35
• [Journal Article] Immortalization of cementoblast progenitor cells with Bmi-1 and TERT. (2005)
  • Author(s): Saito, M.,* Handa, K., Kiyono, T., Hattori, S., Yokoi, T., Tsubakimoto, T., Harada, H., Noguchi, T., Toyoda, M., Sato, S. and Teranaka, T
  • Journal Title: J Bone Miner Res Volume: 20(1) Pages: 50-57
• [Journal Article] Alveolar Bone Marrow as a Cell Source for Regenerative Medicine: Differences between Alveolar and Iliac Bone Marrow Stromal Cells (2005)
  • Author(s): Matsubara, T., Suardita, K., Ishii, M., Sugiyama, M., Igarashi, A., Oda, R., Nishimura, M., Saito, M., Nakagawa, K., Yamanaka, K., Miyazaki, K., Shimizu, M., Tsuji, K., Nakamura, K. and Kato, Y.*
  • Journal Title: J Bone Miner Res Volume: 20(3) Pages: 399-409
• [Presentation] 細胞外マトリックス補充療法による新規歯周病治療技術の開発 (2012)
  • Author(s): 齋藤正寛
  • Organizer: 第12回日本外傷歯学会総会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2012-10-13
• [Presentation] 細胞外マトリックス補充療法による新規歯周病治療技術の開発 (2012)
  • Author(s): 齋藤正寛
  • Organizer: 第6回日本再生歯科医学会シンポジウム
  • Place of Presentation: 帯広
  • Year and Date: 2012-10-07
• [Presentation] Forefront of periodontal regeneration therapy (2011)
  • Author(s): Saito,M.and Tsuji,T
  • Organizer: ISBB meeting Taipei
  • Place of Presentation: Taiwan
  • Year and Date: 2011-07-08
• [Presentation] 抗加齢医学における歯の再生の役割 (2011)
  • Author(s): 齋藤正寛、辻 孝
  • Organizer: 第11回抗加齢学会
  • Place of Presentation: 京都
  • Year and Date: 2011-05-27
• [Presentation] Forefront of periodontal regeneration therapy (2011)
  • Author(s): Masahiro Saito
  • Organizer: ISBB
  • Place of Presentation: Taipei
• [Presentation] ADAMTSL6βによるTGF-βシグナル抑制効果に関する研究 (2011)
  • Author(s): 黒河みさ紀、荻野光明、大島正充、辻 孝、齋藤正寛
  • Organizer: 第135回日本保存学会秋期学術大会
  • Place of Presentation: 大阪
• [Presentation] Present State and Progression of Periodontal Regeneration Therapy
  • Author(s): Masahiro Saito
  • Organizer: IADR general session
  • Place of Presentation: Seattle USA
• [Book] "Tooth regeneration therapy" as a next generation of regenerative medicine., Hiromasa Yoshie, At the forefront. Illustrated Topics in dental research and clinical practice (2012)
  • Author(s): M.Saito.,and T. Tsuji
  • Publisher: Quintessence Publishing Co, Inc., Hanover Park
• [Book] AT THE FOREFRONT ILLUSTRATED TOPICS IN DENTAL RESEARCH AND CLINICAL PRACTICE (2012)
  • Author(s): Saito, M
  • Total Pages: 108
  • Publisher: Quintessence. Publishing Co, Inc