Elucidating the novel functions of Ca2+-dependent phosphorylation pathway during neuronal circuit formation
Project/Area Number |
23680040
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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Keywords | カルシウム / 神経回路形成 / CaMK / 神経回路 |
Outline of Final Research Achievements |
During neuronal circuit formation, spontaneous calcium transients are believed to modify neuronal circuit formation, even before the synaptic maturation. However, how and which Ca2+-dependent downstream molecular events regulate each step of circuit formation are poorly understood. We previously found that distinct limbs of the CaMKK-CaMKI cascade were specifically implicated in determining the extent of either dendritic or axonal growth downstream of different extracellular signals in cultured cortical neurons. In order to extend these results, we have established conditional knockout lines to proceed in vivo study focusing on neuronal circuit formation. We found temporal and cell-type specific knock out of a CaMKI isoform in the developing cerebral cortex indeed impaired circuit formation in vivo and elucidated morphological abnormality of the neurons lacking the CaMKI isoform.
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Report
(5 results)
Research Products
(18 results)
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[Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2.2015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
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Journal Title
Nat Methods
Volume: 12
Issue: 1
Pages: 64-70
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Region-specific activation of CRTC1-CREB signaling mediates long-term fear memory2014
Author(s)
Nonaka M, Kim R, Fukushima H, Sasaki K, Suzuki K, Okamura M, Ishii Y, Kawashima T, Kamijo S, Takemoto-Kimura S, Okuno H, Kida S, Bito H
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Journal Title
Neuron
Volume: 84
Issue: 1
Pages: 92-106
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Signaling from synapse to the nucleus and imaging of active neuronal ensembles2014
Author(s)
Bito H, Inoue M, Nonaka M, Yagishita-Kyo N, Kawashima T, Suzuki K, Ishii Y, Fujii H, Endo T, Goto M, Koyama H, Takemoto-Kimura S, Kim R
Organizer
第37回日本神経科学大会
Place of Presentation
横浜
Year and Date
2014-09-11
Related Report
Invited
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