| Budget Amount *help |
¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
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| Research Abstract |
We aimed to understand how L5 neuron dendrites work in vivo. For this purpose, we developed a fast 3-dimentional scanning 2-photon microscope to achieve dendritic Ca^<2+> imaging with optogenetical manipulation, and local drug applications to a brain area. Dendritic Ca^<2+> activity could be evoked by hindlimb stimulations and electrical stimulations to a higher-order motor cortex in anesthetized mice. This dendritic Ca^<2+> activity significantly increased during awake state. We also found that enlarged dendritic activity significantly suppressed by local application of TTX, a antagonist of sodium channel, to the higher-order motor area. These results indicate that large dendritic activity in awake state can be activated by the higher-order motor area.
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