| Budget Amount *help |
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
Although somatic cell nuclear transfer (SCNT) is sole reproductive engineering technique to allow us to produce individuals from somatic cells, their developmental efficiency is usually less than 5%. In this study, we aimed at practical applications of this technique and attempted to develop procedures of gene regulations by non-genetic modification to improve low developmental efficiency of SCNT. As a results, we obtained following conclusions. (1) mRNA introduction of a transcription factor activated in fertilized embryos could improve developmental efficiency of SCNT embryos in vitro. (2) RNA knock down of Xist gene that triggered X chromosome inactivation in female somatic cells could remarkably ameliorate impaired developmental efficiency into term offsprings of SCNT embryos.
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