|Budget Amount *help
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|Outline of Final Research Achievements
Although somatic cell nuclear transfer (SCNT) is sole reproductive engineering technique to allow us to produce individuals from somatic cells, their developmental efficiency is usually less than 5%. In this study, we aimed at practical applications of this technique and attempted to develop procedures of gene regulations by non-genetic modification to improve low developmental efficiency of SCNT. As a results, we obtained following conclusions. (1) mRNA introduction of a transcription factor activated in fertilized embryos could improve developmental efficiency of SCNT embryos in vitro. (2) RNA knock down of Xist gene that triggered X chromosome inactivation in female somatic cells could remarkably ameliorate impaired developmental efficiency into term offsprings of SCNT embryos.