| Budget Amount *help |
¥28,210,000 (Direct Cost: ¥21,700,000、Indirect Cost: ¥6,510,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
|
|---|
| Research Abstract |
We envisioned the development of an integrated transistor based device that is able to quantify enzymatic activities in cells. Caspase-3 and -8 were studied as targets relevant to apoptosis pathway analysis. A series of peptides containing each Caspase-specific amino acid sequence along with spacer and cysteine moiety were prepared so that they will have good enzyme-accssibility and affinity to gold for the immobilization. Mass-spectroscopic analysis uncovered that these designed reactions take place in quantitative and each substrate-specific fashion. This reaction could also be visualized on the basis of the coloration of gold nano-particle. Furthermore, it was compatible with potentiometric detection format, indicating the validity of our approach.
|
