Development of Integrated Transistor Based device for Monitoring of Enzymatic Activities as Next-generation Platform for Apoptosis Pathways Analysis
| Project/Area Number |
23680051
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
MATSUMOTO Akira 東京医科歯科大学, 生体材料工学研究所, 准教授 (70436541)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥28,210,000 (Direct Cost: ¥21,700,000、Indirect Cost: ¥6,510,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
|
|---|
| Keywords | 電界効果トランジスタ / アポトーシス / カスパーゼ / 酵素活性 / パスウェイ解析 |
|---|
| Research Abstract |
We envisioned the development of an integrated transistor based device that is able to quantify enzymatic activities in cells. Caspase-3 and -8 were studied as targets relevant to apoptosis pathway analysis. A series of peptides containing each Caspase-specific amino acid sequence along with spacer and cysteine moiety were prepared so that they will have good enzyme-accssibility and affinity to gold for the immobilization. Mass-spectroscopic analysis uncovered that these designed reactions take place in quantitative and each substrate-specific fashion. This reaction could also be visualized on the basis of the coloration of gold nano-particle. Furthermore, it was compatible with potentiometric detection format, indicating the validity of our approach.
|
Report
(4 results)
Research Products
(48 results)
